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Citations for R&D Systems :
5301 - 5350 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Primary antibodies used were polyclonal sheep anti-NOTCH3 ECD (SF1559, R&D systems, Minneapolis, MN, USA, used 1:500), monoclonal mouse anti-NOTCH3 ECD (1E4 ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were prepared following the manufacturer’s instructions and incubated with 1 ml of Chemi Reagent Mix (R&D Systems) for 1 min ...
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bioRxiv - Developmental Biology 2024Quote: ... S7 medium was modified by supplementing it with 1 x N21-MAX insulin-free supplement (R&D SYSTEMS, AR010) during S7 (S7D1-S7D14).
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bioRxiv - Microbiology 2021Quote: ... Bacterial cells were then incubated with 0.25 μg of purified human SP-D (R&D Systems, 1920-SP-050), ZAG (R&D Systems ...
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bioRxiv - Immunology 2021Quote: Cytokines were assessed from clarified supernatant using R&D Quantikine ELISA kits for TNFα (R&D Systems, Cat#MTA00B) and DuoSet ELISA kits for IFNβ (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... antibody for flow cytometry was from Abcam and goat polyclonal PTP1B (R and D Systems Cat# AF3954, RRID:AB_2174947) antibody for immunoblotting was from R&D Systems. Recombinant human IL-2 ...
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bioRxiv - Microbiology 2022Quote: Secretion of CXC motif chemokine ligand 10 (CXCL10/IP-10, DY266) and CC chemokine ligand 5 (CCL5/RANTES, DY278) were quantified by DuoSet® ELISA (R&D systems, Bio-Techne GmbH, Wiesbaden, Germany) under BSL-3 conditions according to the manufacturer’s instructions using the appropriate ancillary reagent kit (DY007).
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bioRxiv - Neuroscience 2020Quote: ... 3-6 consecutive sections were prepared from each sample and immunostained with anti-Iba1 antibody (Wako; Richmond, VA; 1:3000) and anti-CD31 antibody (R&D Systems; Minneapolis, MN; 1:200). Images of the ventricular and subventricular zones at the level of the frontal cortex were acquired on Leica SP8 confocal microscope using a 40X (1.3NA ...
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bioRxiv - Neuroscience 2021Quote: ... the IGF-1 concentration in the medium was measured using the Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA)
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bioRxiv - Neuroscience 2024Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
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bioRxiv - Cancer Biology 2024Quote: 6–8-week-old mice were subcutaneously injected with 900 mechanically disrupted KPT GC organoids in 1:1 PBS and RGF BME (R&D Systems, Cat#3533-005-02) vehicle (equivalent of approximately 100,000 cells ...
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bioRxiv - Immunology 2021Quote: ... Slides were then incubated in permeabilization buffer containing the primary REG3β antibody (1:250 dilution; R&D systems, Minneapolis, MN) overnight (16 h) ...
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bioRxiv - Developmental Biology 2020Quote: ... At day 1 the medium was changed to base media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with a mixture of primary goat anti-ACE2 at 1/500 dilution (R&D Systems, #AF3437) and rabbit anti-GnRH (gonadotropin releasing hormone ...
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bioRxiv - Physiology 2020Quote: ... Postprandial plasma obtained at termination of studies (see above for details) was used to measure IGF-1 (R&D Systems), Activin-A (R&D Systems) ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1 ...
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bioRxiv - Physiology 2021Quote: ... CCL2 levels in urine specimens were quantified using Human CCL2/MCP-1 Quantikine® ELISA Kit (R&D systems, DCP00) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Afterwards cells were incubated with primary antibody against PDGFRa (goat, 1:200, AF-307-NA, R&D Systems, MN, USA), CD90 (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... For proximity ligation assays VE-cadherin was detected using mouse VE-cadherin antibody (1:200, R&D systems, Goat, AF1002). c-Src was detected using anti-Src (GD11 clone ...
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bioRxiv - Immunology 2022Quote: ... TNF and IL-1 receptor antagonist (IL-1Ra) were measured in thawed supernatants using commercial ELISA kits (R&D Systems). IL-1Ra was measured because it inhibits the binding and bioactivity of IL-1β85 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems, UK). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Cancer Biology 2020Quote: ... 8 μm cryosections were fixed in acetone and incubated with goat-anti-mouse CD31 (R&D system, #AF3628, 1:200) and rabbit-anti-FITC (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody information is as following: Goat anti-mouse aminopeptidase N/ANPEP (CD13; R&D systems; AF2335; 1:100), Rat anti-mouse vascular adhesion molecule (VCAM1 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and dilutions used for immunohistochemistry in this study were the following: PDGFRA (goat, 1:70; R&D Systems); GPNMB (goat ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with HRP-conjugated IgG Secondary antibody (anti-goat, 1:5000; # HAF017, R&D Systems, goat anti-rabbit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then resuspended in 20 μl of 1× ubiquitin reaction buffer containing 200 nM UBE1 E1 (R&D systems, U-110), 1 μM UbcH5a E2 (Enzo ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-β1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Developmental Biology 2021Quote: ... At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
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bioRxiv - Pathology 2023Quote: CCL2 was quantified in conditioned media from HAoSMCs using Human CCL2/MCP-1 Quantikine ELISA Kit (DCP00, R&D systems) according to the manufacture’s protocol.
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bioRxiv - Immunology 2022Quote: ... permeabilized with ice-cold acetone and stained with 1:200 dilution of monoclonal-anti-RBD (R&D Systems, MN, USA), polyclonal-anti-S1- ...
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bioRxiv - Immunology 2023Quote: ... and in bone marrow plasma using the Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... mice were given SL-TBI (550cGy) and subsequently received intraperitoneal injections of 1 mg/kg UTPγS (R&D systems, 3279), or 1x PBS as control ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence staining: goat anti-mouse VE-cadherin (R&D Systems, AF1002; 1:200), hamster anti-mouse PDPN (Developmental Studies Hybridoma Bank ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted at 1:1000 in TBST 0.1 % supplemented with 2.5 % BSA or with Goat anti- GAPDH antibody (AF5718, R&D systems) diluted at in TBST-0.1 % supplemented with 2.5 % milk or anti-V5 HRP (Thermofisher scientific ...
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bioRxiv - Microbiology 2023Quote: ... The following secondary antibody dilutions were made 1:2000 in PBST: goat α-mouse IgG-HRP (R&D Systems; HAF007) and goat α-rabbit IgG-HRP (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used for immunoblotting: Goat IgG against VE-cadherin (1:500, AF1002, R&D Systems, Minneapolis, MN), pY658 VEC (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used for our IHC study: Goat anti-Serpina3n (1:3000, R&D System, Cat# AF4709, RRID:AB_2270116); Mouse anti-CC-1 (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were first differentiated into macrophages with 50 nM Phorbol-12-Myristate-13-Acetate (PMA) (R&D systems) for 72 hrs ...
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bioRxiv - Immunology 2023Quote: ... Samples were diluted 1:10 and IL8 was measured using the DuoSet IL8 ELISA kit (R&D Systems cat# DY208) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary OC-CAFs were incorporated into a 3D matrix made of rat tail type 1 collagen (R&D Systems) as previously described 95 ...
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bioRxiv - Developmental Biology 2022Quote: ... ITGA8 was stained by the use of polyclonal goat anti-Integrin alpha 8 antibody (1:50; R&D Systems, Minneapolis) and GDNF was stained by the use of polyclonal rabbit anti-GDNF antibody (1:100 ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The following secondary antibodies were used at a 1:5000 dilution: goat anti-rabbit IgG-HRP (R&D Systems; HAF008) and goat anti-mouse IgG-HRP (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: The levels of INFγ were evaluated in supernatants obtained from 0.625:1 and 1.25:1 effector:target ratio overnight co-cultures of CAR T-cells:CAFs using the Human IFN-Gamma Quantikine Kit (R&D Systems) following manufacturer’s instructions ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... antigen-retrieved in pH6 sodium citrate buffer and stain with primary antibodies including: anti-LYVE-1 (AF2089; R&D System), anti-CXCL8 (MAB208 ...
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bioRxiv - Physiology 2024Quote: ... 24 well-plates were coated with recombinant murine (rm) E-selectin (rmCD62E-Fc chimera, 10µg mL-1, R&D Systems) or PBS/0.1% BSA at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody (1:100 ...