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Citations for Santa Cruz :
651 - 700 of 7673 citations for 8 Chloro 2 methylimidazol 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged to remove debris and the supernatant was rocked at 4°C for 2 h with an anti-Flag antibody (anti-DDDDK tag, MBL Life Science) or mouse anti-IgG antibody (Santa Cruz Biotechnology) that was conjugated with ‘Dynabeads Protein A’ (Veritas ...
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bioRxiv - Bioengineering 2023Quote: ... Alomone Labs) or 5 µM of antibodies for targeting neurons with dopamine receptor 2 (D2R) (anti-D2DR, sc-5303, Santa Cruz Biotechnology) or without cell type specificity (rabbit anti-human IgG ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cell pellets were then incubated with room temperature PBS containing 2 mM DSG (disuccinimidyl glutarate, sc-285455, Santa Cruz Biotechnology) for 25 min with gentle agitation ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was separated by centrifugation at 12.000 g at 4 °C for 10 min and incubated with STAT2 antibody (2 µg) (Santa Cruz Biotechnology, 514193) for 3 h at 4°C with gentle shaking ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were detected by immunoblotting with anti-PLCβ1 (D-8, Santa Cruz) and anti-Ago2 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal α-Vsp35 and mouse monoclonal α-8-OHdG (Santa Cruz); goat polyclonal α-Vsp35 and mouse monoclonal α-dsDNA (Abcam) ...
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bioRxiv - Cell Biology 2019Quote: ... anti-VE-Cadherin (Santa Cruz clone F-8 cat#sc9989 lot E1018)(Fig.5A and B) ...
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bioRxiv - Immunology 2022Quote: ... mouse monoclonal anti-Xinα [D-8] (Santa Cruz Biotechnology, Inc; sc-166658) 1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence of 8 μg/ml polybrene (Santa Cruz, sc-134220A), and at a multiplicity of infection of approximately 0.3 ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence of 8 μg/ml polybrene (Santa Cruz, sc-134220A) and subsequent selection with 10 µg/ml blasticidin (InvivoGen ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Immunology 2021Quote: ... JUND (D-9, sc-271938), SMAD3 (38-Q, sc-101154), MAX (H-2, sc-8011), and MYC (9E10, sc-40) (all, Santa Cruz Biotechnology, USA) followed by peroxidase-conjugated anti-mouse antibodies (NA931 ...
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bioRxiv - Molecular Biology 2020Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...
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bioRxiv - Molecular Biology 2020Quote: ... were washed with 200 μL HBS (150 mM NaCl, 10 mM HEPES, pH7.5 by KOH) and incubated with 2 μg anti-HA (Santa Cruz, Cat.# sc-7392) or 2 μg rabbit IgG isotype (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cas9-expressing cell lines were infected at a density of 2×105 cells in 1.5 mL media in the presence of 4 μg/mL polybrene (Santa Cruz Biotechnology, SC-134220). sgRNA resistant human ZRSR2 DNA fragment was synthesized by Twist Bioscience and cloned into pRRL.idTomato.
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 5% skimmed milk powder for 2 hours at room temperature.Incubate primary antibody overnight at 4°C.The membrane was washed three times with TBST and then incubated at room temperature with conjugated HRP secondary antibodies for 2 hours.ECL (enhanced chemiluminescence kit, Santa Cruz Biotechnology, Dallas, TX) detection system (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... RNA Pol II and NF-YA levels were measured using an anti-Rpb3 antibody60 and anti-NF-YA antibody (G-2; Santa Cruz, sc-17753X) respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight rotating at 4 °C together with either α-KAP1 or α-RIF1 antibodies (see Supplementary Table 2) or IgG only control (#sc-2026, Santa Cruz), 10% of chromatin was isolated as input control ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were passaged as needed in the presence of 2 μM Rho-associated kinase (ROCK) inhibitor (RI) thiazovivin (Santa Cruz Biotechnology, #SC-361380). Prior to transfection ...
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bioRxiv - Genetics 2022Quote: ... Once the cells were ∼50% confluent (after 2-3 days) the media was changed to StemFlex with 1ug/uL polybrene (Santa Cruz SC-134220), and 10uL each of Ngn2 and rtTA virus was added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate PARP14 by incubating with 2 µg of a mouse monoclonal antibody against PARP14 (Santa Cruz, sc-377150), and protein G agarose beads overnight at 4°C with gentle mixing on a nutator at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... catalog number sc7392), α-GFP (clone B-2, catalog number sc9996), and α-ubiquitin (clone P4D1, catalog number sc8017) antibodies were purchased from Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... α-ISP1 (8), α-SAG1, α-Ty, α-MIC2, ROP2-4 (gifts from J-F Dubremetz, Montpellier) and acetylated α-tubulin (6-11B-1; Santa Cruz Biotechnology). Tubulin antibodies AA344 scFv-S11B (β-tubulin ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibody against 8-OHdG (clone 15A3, Santa Cruz Biotechnology, Dallas, USA) was used at a dilution of 1:200.
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bioRxiv - Neuroscience 2020Quote: ... and reprobed with mouse monoclonal CSF-1R antibody (D-8, Santa Cruz Biotechnology), anti-AKT (Cell signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were incubated with anti-TOP2B (H-8, Santa Cruz, Dallas, TX) and anti-phospho-BRCA1 (Ser1524 ...
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bioRxiv - Molecular Biology 2021Quote: 300 million TetR-CBX7 reporter mESCs were infected with a 1:10 dilution of the harvested virus-containing supernatant PlatinumE cell medium for 24 hrs in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). The 300 million cells were divided into 3x 100 million sets (10x 15 cm plates of 10 million cells each ...
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bioRxiv - Biophysics 2020Quote: ... using EZ-Retriever V.2 (BioGenex, San Ramon, CA, USA) and immunostained with anti-α-SMA monoclonal antibody (Cat No.sc-32251, Santa Cruz Biotechnology, CA, USA) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and β5-integrin cells were incubated for 20 min before chase and during pulse with 10 μM gefitinib (Santa Cruz Biotechnology, 184475-35-2), 10 μM PP2 (Thermo-Scientific ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates of equal protein amounts were incubated overnight at 4°C (constant rotation) with 2 μg of an anti-HA antibody or immunoglobulin G (IgG) control (sc2027; Santa Cruz Biotechnology, Dallas, TX). Co-immunoprecipitation of STAT3 with associated proteins was then performed by using Protein A/G Agarose beads included in the Pierce Classic IP Kit (26146 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were blocked in 3% BSA in PBS with 0,2% Tween 20 and incubated overnight with anti-MYC (A-14, Santa Cruz Biotechnology, 2 ug/ml), anti-CENP-C (PD030 ...
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bioRxiv - Immunology 2023Quote: ... Lungs were dissected at the stated time post tumor initiation and fixed for 24 hours in 2% paraformaldehyde (Santa Cruz Biotechnology Cat# sc-253236) at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed from dishes and assessed by flow cytometry for construct expression and selected in puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B). Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with PB-vector constructs as above ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunofluorescence were Rat Anti-C-peptide (Developmental Studies Hybridoma Bank, ID-4) and Goat Anti-GLP-2 (Santa Cruz Antibodies, C-20).
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bioRxiv - Cell Biology 2019Quote: ... Western blotting: anti-VE-Cadherin (Santa Cruz clone F-8 cat#sc9989 lot E1018), anti-VEGFR2 (Cell Signaling clone D5B1 Cat#9698 and Cat#2479) ...
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bioRxiv - Cell Biology 2019Quote: ... 100 μl virus-containing supernatant supplemented with 8 μg/ml polybrene (Santa Cruz Biotechnology) was added to 30,000 cells ...
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bioRxiv - Cancer Biology 2019Quote: ... The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA) and Bcl-2 (mouse monoclonal ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing IgG to the SARS-CoV-2 nucleoprotein (NP) and with mouse anti-human GAPDH monoclonal antibody (G-9: Santa Cruz Biotechnology, Dallas, TX, USA). The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma ...
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bioRxiv - Genomics 2021Quote: ... iPSC-derived MSCs were cultured to at least passage 6 and seeded at 4.2 × 104 cell/cm2 in culture dishes (4-10cm diameter) coated in 5ug/cm2 Type I Collagen (sc-136157, Santa Cruz Biotechnology, Dallas, TX, USA). After culturing cells for 1 day in MSC medium ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated for 1h at room temperature in blocking buffer (5% nonfat milk in PBS pH 7.4 containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 h at room temperature with antibodies raised against insulin (Santa Cruz Biotechnology Inc, Santa Cruz, CA), the porosome associated protein ATP2C1 and actin (Santa Cruz Biotechnology Inc ...
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... On the day of the transfection two separate solutions were prepared: Solution A was made by adding 2 μg of plasmid DNA (Santa Cruz Biotechnology ®, sc-405646) into 130 μl of plasmid transfection medium (Santa Cruz Biotechnology ® ...
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bioRxiv - Immunology 2023Quote: ... Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3, VDR, Gfi1) using protein G agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA). Eluted conjugates were run on SDS-PAGE and probed with primary antibodies of partner proteins ...
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bioRxiv - Biophysics 2024Quote: ... TDP-43 aggregation levels were detected by GFP fluorescence signal and immunoblotting for GFP using α-GFP (B-2) primary antibody (Santa Cruz Biotechnology, sc-9996) with α-mouse IgG secondary antibody (MBL).
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bioRxiv - Immunology 2023Quote: Chromatin from flow-sorted small pre-B cells (2×106) from WT and Brwd1-/- mice were used for each ChIP experiment with anti-BRWD1 (E-15, Santa Cruz Biotechnology, sc-83517, lot J1508), anti-H3K4me1 (07-436 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... prior to immunoblotting using the following primary antibodies: COX4 (F-8, sc-376731, Santa Cruz), GSTA1 (R-14 ...