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Citations for Santa Cruz :
4451 - 4500 of 4534 citations for Recombinant Mouse IGHG1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles through harvesting cell culture supernatants and by using immunoblot analysis with a mouse monoclonal anti-HTLV p24 antibody (Catalogue #: sc-53891; Santa Cruz Biotechnology, Dallas, TX). To determine particle production (i.e. ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 in PBS for 1 h at room temperature followed by overnight incubation at 37 °C with mouse anti-MYO7A (myosin VIIA) (1:200, Santa Cruz Biotechnology, sc-74516). Cochlear pieces were then incubated with fluorophore-conjugated species-appropriate secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: The following antibodies were used in the indicated dilution or amount: Mouse anti-AR (WB: 1:500; IF: 1:200; IP: 1.5 µg; Santa Cruz Biotechnology; Cat# sc-7305). Mouse anti-ERα (WB ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with Alexa Fluor 647-conjugated anti-NS1 mouse antibody (1:500 dilution, sc-130568 AF647; Santa Cruz Biotechnology, Dallas, TX, USA) for 1 h at room temperature subsequent to all incubation processes ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000 µg of total protein suspended in 500 µL of cell lysate was precleared with 20 µL of normal mouse IgG-conjugated agarose beads (Santa Cruz Biotechnology; sc-2343) for 30 mins at 4°C on a rotator ...
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bioRxiv - Immunology 2024Quote: ... Membranes were blocked in 5% dried milk/TBS-T buffer and incubated over night with anti-mouse TREX1 antibody (clone C-11, Santa Cruz Biotechnology, Dallas, TX) and and anti-β-tubulin polyclonal antibody (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... Antibody cross-reactivity was accounted for via probing non-incubated LC3C blots with mouse anti-HSp27 antibody (Santa Cruz Biotechnology, sc-13132; 1:1000). All Far Western blots were performed at least in duplicate.
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bioRxiv - Cell Biology 2024Quote: ... and incubated with rabbit anti-podocin 1:150 (IBL International, Germany) and mouse anti-integrin α3 conjugated with Alexa Fluor 647 1:500 (Santa Cruz Biotechnology, Dallas, USA) and anti-rabbit Alexa Fluor 488-conjugated IgG 1:600 (ChromoTek ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: Protein expression was quantified by western blot analysis using the following antibodies: anti-cyclin A (1:500 sc-751; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Histone H3 (phospho Serine10 ...
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bioRxiv - Zoology 2019Quote: Protein was extracted from 5 live larvae using the methanol/chloroform protocol [33] and solubilized in electrophoresis sample buffer (Santa Cruz Biotechnology, Dallas, TX, USA) for SDS-polyacrylamide gel electrophoresis and Western Blot analysis ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... 500-1000ug protein extracts were incubated under rotary agitation overnight at 4 °C with antibodies against SMARCA4 (Santa Cruz Biotechnology, sc-374197 X; 4 µg), YAP (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2019Quote: Coimmunoprecipitation was performed as previously described (Waxman, Baconguis, Lynch, & Robinson, 2007) with the following substitutions: Protein A/G agarose (Santa Cruz Biotechnology, Santa Cruz, CA) was used to bind 3ug of antibody ...
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bioRxiv - Molecular Biology 2020Quote: Cellular protein was collected with a low-salt lysis buffer and was subjected to immunoblotting analysis with GAPDH (1:4000, Santa Cruz Biotechnology Inc., CA, USA) or iNOS (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% milk/TBST for 30 min and incubated with primary antibodies/TBST against proteins of interest: anti-RBM12 (Santa Cruz Biotechnology, Cat. #sc-514258), anti-PKA alpha polyclonal antibody (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... and the resulting pellet was evaluated for the presence of specific protein marker of EVs (endosome pathway marker): ALIX (1:750, sc49267, Santa Cruz Biotechnology, Dallas, TX, USA). As negative marker was used the proteins GRP78 (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... 200 µL of chromatin (from approximately 7×106 MCF10A cells) were pre-cleared per condition by incubating with Protein A/G beads (Santa Cruz #SC-2003. Lot#B2323) for two hours at 4°C on a rotator ...
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bioRxiv - Immunology 2024Quote: ... Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3, VDR, Gfi1) using protein G agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA). Eluted conjugates were run on SDS-PAGE and probed with primary antibodies of partner proteins ...
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Tumour lysates were subjected to SDS-PAGE followed by protein transfer and immunoblotting with primary antibodies (anti EPLIN, Novus Cat NB100-2305 and anti β-actin, Santa Cruz Cat sc-47778). Signal amplification was performed with HRP-conjugated secondary antibodies (Goat Anti Mouse IgG and Anti Rabbit IgG von Southern Biotek ...
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bioRxiv - Biophysics 2021Quote: ... The primary antibodies used for the immunostaining were mouse monoclonal antibodies (Arp3 [A-1], sc-48344; formin 2 [C-3], sc-376787; Santa Cruz Biotechnology, Dallas, Texas, USA). The primary antibodies were diluted 1:200 in 3% BSA and left in contact with the cells for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Expression of human ICAM-1 and VCAM-1 expression were evaluated by SDS PAGE using mouse anti-human I-CAM1 antibodies (Santa Cruz Biotechnology, Cat#sc-8439) and rabbit anti-VCAM-1 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min at RT and incubated with the following primary antibodies at 4°C overnight in TBST with 5% BSA: monoclonal mouse GFP antibody (B-2: sc-9996, Santa Cruz Biotechnology, Inc., 1:500), polyclonal pan-XIRP2 antibody (custom generated (see above) ...
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bioRxiv - Physiology 2020Quote: ... Leptin receptor and its phosphorylated domains were evaluated using the following antibodies: mouse monoclonal (MM) against leptin receptor (ObR; 1:500, cat# sc-8391, Santa Cruz Biotechnology, Dallas, Texas, USA), goat polyclonal (GP ...
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bioRxiv - Cancer Biology 2019Quote: ... The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA) and Bcl-2 (mouse monoclonal ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were stained overnight at 4°C using unconjugated primary antibodies at 1:100 dilution for endomucin (rat anti-mouse sc-65495, Santa Cruz Biotechnology, Santa Cruz, CA) and at 1:200 dilution for collagen type I (rabbit anti-mouse ...
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bioRxiv - Neuroscience 2019Quote: ... Remaining unlabelled primary antibody-bound surface HA-Nrxn1α was blocked with overnight incubation with Fab fragments anti-mouse [0.13 mg/mL (Santa Cruz Biotechnology, Cat #715-007-003) in 5% (wt/vol ...
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bioRxiv - Systems Biology 2019Quote: ... Cells are then incubated overnight at 4°C with monoclonal primary antibody as following: mouse anti-Hsc70 (1:100 dilution; Santa Cruz Biotechnology, Dallas, Texas, USA), or mouse anti-HSP72 (1:100 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubating with primary antibodies overnight (ON) at 4°C (mouse anti-aSyn antibody (211) 1:100 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA); rabbit anti-vimentin 1:100 (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing IgG to the SARS-CoV-2 nucleoprotein (NP) and with mouse anti-human GAPDH monoclonal antibody (G-9: Santa Cruz Biotechnology, Dallas, TX, USA). The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... PV-235; 1:1000), rabbit anti-glucose transporter 1 (Glut1, Frontier Institute, Af1020; 1:1000) and mouse anti-tubulin (Santa Cruz, sc-32293; 1:10000). After washing three times with TBST ...
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:10000 in 5% non-fat dry milk in PBST1X and incubated 1h at RT and subsequently with Goat anti-mouse-HRP (Santa Cruz, Dallas, TX, USA-A1303) diluted 1:1000 in 2% non-fat dry milk in PBST1X 30min RT.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were blocked for 3h at RT and incubated with primary antibodies (rabbit anti-mouse NF-κB(RelA/p65/) (sc-8008; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-mouse phospho(p)-RelA/p65(NF-κB ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane is washed three times with PBST and incubated for 1 h at 25 °C with an anti-mouse HRP conjugated with a secondary antibody (sc-2005, Santa Cruz Biotechnology, Dallas, TX, USA) diluted 1:10,000 (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... blots were rinsed using TBST and incubated with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (Millipore, cat no. AP127P for mouse primary ab and Santa Cruz Biotechnology cat# sc-2005) for rabbit primary ab ...
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bioRxiv - Immunology 2022Quote: ... Antibody expression and purification were evaluated using polyacrylamide gel electrophoresis stained with Coomassie Brilliant Blue G-250 and Western-blot using a mouse anti-HA (Santa Cruz Biotechnology, cat: sc-7392) at a dilution of 1:1,000 ...
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bioRxiv - Bioengineering 2020Quote: ... at room temperature for 1 hour and then incubated with a mouse monoclonal HA antibody (F-7) (1:500 dilution; catalog no. sc-7392; Santa Cruz Biotechnology, Dallas, TX, USA) or mouse monoclonal β-actin antibody (1:1000 dilution ...
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bioRxiv - Genetics 2022Quote: ... and then 1 hour at room temperature with the HRP linked secondary antibody (HRP-linked anti-rabbit IgG, #7074 Santa Cruz, dilution 1/2000; HRP-linked anti-mouse IgG, #7076 Santa Cruz, dilution 1/2000). Signal was detected with Amersham ECL select substrate (Cytiva ...
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bioRxiv - Pathology 2020Quote: ... at 95 °C for 36 min followed by antibody incubation at 37 °C for 32 min using the mouse monoclonal antibody clone J-13 (Santa Cruz Biotechnology, Inc., Dallas, USA) at 1:50 dilution ...
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bioRxiv - Biochemistry 2020Quote: The following antibodies were used for western blotting (WB) and immunofluorescence (IF): mouse anti-beta-actin (Santa Cruz, catalogue number sc-47778; WB 1:2000), mouse anti-HA (ENZO ...
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bioRxiv - Bioengineering 2019Quote: ... SOTRs were then incubated with the primary antibodies anti-troponin T2 (TnT2; mouse monoclonal IgG; 1:200; SC-20025; Santa Cruz Biotechnology, Dallas, TX, USA), anti-a-actinin (mouse monoclonal IgG ...
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bioRxiv - Developmental Biology 2019Quote: ... the protocol was modified by adding the primary antibody recognizing the target (Krt8, rabbit, Abcam, EP1628Y, 1/100; Aqp1, mouse, Santa Cruz, sc-25287, 1/50) to the anti-DIG-AP antibody mixture and incubation O/N at 4 °C (Lopez ...
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bioRxiv - Neuroscience 2021Quote: ... Western blot analysis was performed with the following primary antibodies: mouse monoclonal anti-D3R (1:100; sc-136170, Santa Cruz Biotechnology Inc., Dallas, Texas, USA); rat monoclonal anti dopamine transporter (DAT ...
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bioRxiv - Neuroscience 2021Quote: ... The reads were mapped with TopHat v.2 software (CCB, Johns Hopkins University, Baltimore, MD, USA) to the UCSC mm10 mouse reference (Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA). Biological quality control and summarization were performed with RSeQC-2.3.3 and PicardTools1.92 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed and incubated for 1 hr at room temperature with the appropriate HRP-labeled pre-absorbed goat anti-rabbit or anti-mouse secondary antibodies (Santa Cruz Biotechnology, Inc.; Dallas, TX). The membranes were washed ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were collected and precleared by incubating with 1 μg of mouse anti-IgG (Cat. No. sc-2025; Santa Cruz Biotechnology [SCB], Dallas, TX) and 20 μL of protein A/G-PLUS agarose beads (Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... MPI-CBG antibody facility and a kind gift from Prayag Murawala) and anti-PCNA (mouse) conjugated with Alexa 647 (Santa Cruz PC10, sc-56 AF647) was done in a blocking solution 1:100 overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Nitrocellulose was incubated in blocking buffer (PBS containing 3% powdered skimmed milk) for 30 minutes at rt and then incubated with primary mouse anti-DOK7 antibody (Santa Cruz cat no sc-390856) diluted 1:250 in blocking buffer for 1 hour at rt with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated overnight at 4◦C or for 1 h at RT in 2%BSA PBS containing primary antibody mouse anti-lamin B1 (Santa Cruz, sc-365214, 1/500). Cells were washed using PBS and incubated for 1 h at room temperature with secondary antibody in 2% BSA PBS ...