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Citations for Santa Cruz :
4301 - 4350 of 4534 citations for Recombinant Mouse IGHG1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... at 4 °C followed by 3–18 h rotated incubation with protein A/G agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: Coimmunoprecipitation was performed as previously described (Waxman, Baconguis, Lynch, & Robinson, 2007) with the following substitutions: Protein A/G agarose (Santa Cruz Biotechnology, Santa Cruz, CA) was used to bind 3ug of antibody ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and expression was detected using the following antibodies: ATF3 (sc-81189, Santa Cruz Biotechnology, Dallas, TX, USA), cleaved caspase-3 (#9664 ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Nucleus proteins of the ANKRD49-OE or LV5 H1299 cells (1×106) were extracted and 25 µg of them were precleared using Protein A/G Agarose (Santa Cruz, California, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates (500 µg) were pre-cleared with 30 µl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of protein were first separated by SDS-PAGE and then blotted with the following antibodies: MSH3 (1:500, Santa Cruz, sc-271080), huntingtin (1:2,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The epitope-tagged Pum and α-tubulin proteins were detected by successive incubation with anti-Myc 9E10 monoclonal primary (SC-40, Santa Cruz Biotechnology) and anti-α-tubulin B-5-1-2 monoclonal primary (T5168 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: LDLR cell surface protein was quantified by fixing cells in 4% paraformaldehyde and incubated with anti-LDLR at 1:100 (Santa Cruz Biotechnology, sc18823) for 45 min ...
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bioRxiv - Cell Biology 2020Quote: ... by washing 3 times with phosphate-buffer saline (PBS) followed by incubation with either 10 µg of mouse monoclonal anti-Ndc80/Hec1 antibody (Santa Cruz, sc-515550) or 10 µg of mouse IgG (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... slides were briefly rinsed with PBS and stained overnight at 4°C with antibodies (mouse anti-EPS8, BD Transduction Laboratories Cat#610144, 1:200; rabbit anti-Villin 1:50, Santa Cruz Cat# SC28283) in 1% NGS in PBS ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to an Immobilon-P membrane (MilliporeSigma) and probed with either mouse-anti MAL 1:1000 (clone E-1, Santa Cruz Biotechnology, Inc.) or rabbit anti-ICln 1:1000 (PA5-13450 ...
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bioRxiv - Biochemistry 2022Quote: ... CST, #12697, D8O3J), anti-β-arrestin2 antibody (rabbit monoclonal; CST, #3857, C16D9) and anti-α-tubulin antibody (mouse monoclonal, clone DM1A; Santa Cruz Biotechnologies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C with different primary antibodies: for HEK cells a mouse monoclonal αTRPA1 antibody (1:1000; Santa Cruz Biotech #376495) was used ...
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bioRxiv - Molecular Biology 2020Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: mouse monoclonal antibody against Proliferation Cell Nuclear Antigen (PCNA, sc56 Santa Cruz, CA, USA, 1:50 dilution) and rabbit polyclonal against myeloperoxidase (MPX ...
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bioRxiv - Genetics 2019Quote: ... Membranes were blocked with 5% milk powder in TBS-T and incubated overnight at 4°C with primary antibodies: anti-GFP mouse antibody (Santa Cruz sc-9996) and anti-PGKD1 mouse antibody (Novex 459250 ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were stained overnight at 4°C using unconjugated primary antibodies at 1:100 dilution for endomucin (rat anti-mouse sc-65495, Santa Cruz Biotechnology, Santa Cruz, CA) and at 1:200 dilution for collagen type I (rabbit anti-mouse ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse α-Myc (9E10, 1:500), rabbit α-SKP1 (H-163, 1:1000) and mouse α-ubiquitin (P4D1, 1:1000) were obtained from Santa Cruz. Mouse α-Nucleophosmin (NPM ...
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bioRxiv - Physiology 2019Quote: ... blocked in 5% BSA for 1 hour at room temperature and incubated overnight at 4°C with rabbit polyclonal anti-ABHD2 (1:300) and mouse monoclonal anti-α-actin (1:500, sc-32251, Santa Cruz Biotechnology). The antibody-antigen complexes were visualized by incubation for 1 hour at room temperature with 1:2000 Alexa Fluor 488-conjugated goat anti-rabbit (Molecular Probes A11008/Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2020Quote: ... at 1:200, anti-monomeric Kusabira-Orange 2 (rabbit, MBL amalgam PM051M) at 1:250 and anti-p63 (mouse, Santa Cruz Biotechnology 4A4) at 1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was washed three times with Tris buffer and incubated for one hour with anti-mouse antibodies conjugated with horseradish peroxidase (Santa Cruz Biotechnology, USA). After washing the membrane ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were reprobed for Gβ by stripping and then incubating overnight with 1:200 diluted mouse monoclonal anti-Gβ antibody (Santa Cruz #sc-166123) followed by a secondary incubation with 1:1000 diluted m-IgGk BP-HRP (Santa Cruz #sc-516102) ...
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bioRxiv - Cancer Biology 2020Quote: The pH of 500 μl mouse IgG1 HPV 16 E6/18 E6-HRP (CP15) monoclonal antibodies (Santa Cruz Biotechnologies, USA Cat# sc-460) was determined using a pH Meter ...
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bioRxiv - Cancer Biology 2020Quote: ... Gal-3 and Ki67 expression was detected by IHC using mouse polyclonal anti-Gal-3 (Santa Cruz, CA, USA, SC-14364, 1:500) and rabbit polyclonal anti-Ki67 (abcam ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated with goat anti-mouse HRP conjugated secondary antibodies (Novex, A16072) and visualized using luminol reagent (Santa Cruz Biotechnology, sc-2048). Membranes were scanned using the GE LAS 4000 imager (GE Healthscience ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 4°C in a humid chamber with 1:200 mouse monoclonal anti-NF-κB p65 antibody (Santa Cruz, CA, USA) in 0.1% Tx-100 ...
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bioRxiv - Microbiology 2021Quote: Triple immunostaining for KS tissues or 3D spheroids implant sections was performed using a mouse anti-PDGFRA (Immunoway, 4G11, YM3688) or anti-Nestin (Santa Cruz, sc-23927) antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with primary antibodies in 0.3% Triton-X and 5% goat serum in PBS at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), rabbit anti-FOXG1 (ab18259 Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Actin was measured as a loading control with a monoclonal mouse anti-actin antibody (1:1000, sc-47778, Santa Cruz; Santa Cruz, CA). Donkey anti-rabbit IR800 and donkey anti-mouse IR680 secondary antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Actin was measured as a loading control with a monoclonal mouse anti-actin antibody (1:1000, sc-47778, Santa Cruz; Santa Cruz, CA). Donkey anti-rabbit IR800 and donkey anti-mouse IR680 secondary antibodies (1:10,000 ...
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bioRxiv - Microbiology 2022Quote: The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles by harvesting cell culture supernatants by using immunoblot analysis with a mouse monoclonal anti-HIV-1 p24 antibody (Catalogue #: sc-69728; Santa Cruz Biotechnology, TX). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was then incubated overnight with the following primary antibodies: mouse anti-Neuregulin1α/β1/2 (D10) (1:500) (#sc-393009; Santa Cruz Biotechnology, USA), rabbit anti-phospho-Akt (Ser473 ...
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bioRxiv - Cell Biology 2022Quote: ... The antibodies and dyes used for ICC are as follows: mouse α-pan 14-3-3 (sc-1657, Santa Cruz Biotechnology, 1:100), mouse α-Flag® M2 (F1804 ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with HRP-conjugated secondary antibodies: donkey-anti-rabbit 1:5000 (Amersham, #NA934V) or goat-anti-mouse 1:5000 (Santa Cruz, #sc-2031), for 1 hour at room temp ...
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bioRxiv - Genetics 2022Quote: The following primary and secondary antibodies were used at the indicated dilutions: Myc (mouse monoclonal, Santa Cruz Biotechnology #sc-40, 1:500, WB); Clb2 (rabbit polyclonal ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then blocked in 10% normal goat serum (NGS) in PBS for 1h at room temperature followed by staining with: mouse anti-ERα (F-10) monoclonal antibody (Santa Cruz, #sc-8002) 1:50 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: a mouse monoclonal anti-HNF-3β/FoxA2 (H-4) antibody sc-374376 (1:300, Santa Cruz Biotechnology, USA), a mouse monoclonal anti-Nkx2.2 antibody 74.5.A5 (1:600 ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed 4 times and incubated with anti-IgG1 mouse-horseradish peroxidase (HRP) conjugate (1:2000 dilution; Santa Cruz Biotechnology Inc.) at RT for 1 h ...