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Citations for Santa Cruz :
2201 - 2250 of 2406 citations for Recombinant Rabbit CCL5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30 °C for 30 minutes or 1 hour before separation by SDS–PAGE followed by immunoblot analysis for MBP-substrate proteins (Anti-MBP-probe Antibody (R3.2) (Santa Cruz sc-53366)).
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bioRxiv - Genomics 2023Quote: ... Samples were pre-cleaned by incubating with 2–4 μg of normal immunoglobulin G (IgG) and 50 μl of A / G-agarose protein microspheres (Santa Cruz Biotechnology) for 1 hour at 4 °C with agitation for preclearance ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were immunoprecipitated using monoclonal mouse anti-WWOX cocktail antibodies53 together with protein A/G plus agarose beads (Santa Cruz, Texas, USA) and incubated overnight at 4°C on a rotor ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed and incubated with mouse IgG kappa-binding protein conjugated to horseradish peroxidase (m-IgGk-HRP, 1:4000) (Santa Cruz technologies) in 5% milk for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated overnight with RIP immunoprecipitation buffer containing magnetic Protein G beads conjugated with human SFPQ/PSF antibody (sc-271796, Santa Cruz, Cliniscience) or negative control mouse IgG (5415S ...
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bioRxiv - Cancer Biology 2023Quote: ... approximately 750 µg of solubilized proteins were incubated with 1 µg of anti-C/EBPβ mouse IgG (Santa Cruz Biotech, sc-7962) or normal mouse IgG (Santa Cruz Biotech ...
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bioRxiv - Immunology 2023Quote: ... 1 mg of hTCEpi cell lysate in cell lysis buffer was precleared with 25 μl of protein A/G plus agarose (SC-2003; Santa Cruz Biotechnology) for 2 hours at 4°C with rotation before the agarose was removed by centrifugation at 2,000 rcf for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... the formation of a fusion protein with a proper size was validated by Western blotting using anti-myc antibody 9E10 (Santa Cruz Biotechnology), and anti-Xpress tag antibody R91025 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to HRP (1:2000) (sc-516102, Santa Cruz Biotechnology) for 3h at 4°C on a rotary and washed thrice with TBST for 10 min each time ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aliquots of lysates containing 100 μg of protein were used for each immunoprecipitation reaction with indicated antibodies followed by adsorption to protein A/G PLUS-agarose beads (Cat#: sc-2003, Santa Cruz Biotechnology). DNA-protein cross-link was reversed by heating the samples to 65 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were then incubated with goat anti-rabbit secondary antibody and visualized using Diaminobenzidine (DAB) staining kit (Santa Cruz Biotechnology, Santa Cruz, USA) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... slides were briefly rinsed with PBS and stained overnight at 4°C with antibodies (mouse anti-EPS8, BD Transduction Laboratories Cat#610144, 1:200; rabbit anti-Villin 1:50, Santa Cruz Cat# SC28283) in 1% NGS in PBS ...
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bioRxiv - Immunology 2021Quote: ... Primary pan-DUOX antiserum (1:1000; provided by Xavier De Deken’s laboratory) or normal rabbit IgG (control; Santa Cruz Biotechnology, Santa Cruz, CA) were applied to sections overnight at RT with slow rotation ...
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bioRxiv - Developmental Biology 2020Quote: ... Cryosections were blocked with 3% BSA/1% normal donkey serum in a humidified slide box and subsequently incubated with anti-Pbx2 (1:100, polyclonal rabbit, Santa Cruz, G-20) and anti-Myod1 (5.8A ...
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bioRxiv - Biochemistry 2022Quote: ... CST, #12697, D8O3J), anti-β-arrestin2 antibody (rabbit monoclonal; CST, #3857, C16D9) and anti-α-tubulin antibody (mouse monoclonal, clone DM1A; Santa Cruz Biotechnologies ...
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bioRxiv - Neuroscience 2021Quote: ... The tissues sections were then incubated with primary antibody (rabbit anti-c-Fos, SC-52; Santa Cruz Biotechnology, 1:1000 in PBS-T) at 4°C for 72 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were rinsed several times in 1xTBSTw and incubated with goat anti-rabbit HRP-conjugated secondary antibody (1:20,000; Bio-Rad, catalog 179-5046; or 1:10,000; Santa Cruz, catalog sc-2004) in 5% non-fat dried milk for 1 hour at room temperature with gentle agitation ...
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bioRxiv - Cell Biology 2019Quote: ... membranes were washed three times for 15 min with PBST and incubated with the secondary antibody donkey anti-rabbit- IgG-HRP (sc-2317, Santa Cruz Biotechnology, USA) at 1:10000 for 1h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse α-Myc (9E10, 1:500), rabbit α-SKP1 (H-163, 1:1000) and mouse α-ubiquitin (P4D1, 1:1000) were obtained from Santa Cruz. Mouse α-Nucleophosmin (NPM ...
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bioRxiv - Physiology 2019Quote: ... blocked in 5% BSA for 1 hour at room temperature and incubated overnight at 4°C with rabbit polyclonal anti-ABHD2 (1:300) and mouse monoclonal anti-α-actin (1:500, sc-32251, Santa Cruz Biotechnology). The antibody-antigen complexes were visualized by incubation for 1 hour at room temperature with 1:2000 Alexa Fluor 488-conjugated goat anti-rabbit (Molecular Probes A11008/Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2019Quote: ... blocked and re-probed with specific Crithidia fasciculata anti-Hsp70 (1:5000, 1 h) (Johnson and Englund, 1998) and secondary chicken anti-rabbit-HRP (1:2000, 1 h) (Santa Cruz, sc-516087). Signal was detected with Clarity™ ECL Blotting Substrate (NEB ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated with horseradish peroxidase (HRP)-conjugated donkey anti-goat or goat anti-rabbit secondary antibody (R&D system and Santa Cruz), before incubation with diaminobenzidine substrate as a chromogen (Dako ...
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bioRxiv - Genetics 2019Quote: ... USA) for 24 hours at 4°C or rabbit anti glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) (1:1000, Santa Cruz, Dallas, TX, USA) for 3 hours at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... at 1:200, anti-monomeric Kusabira-Orange 2 (rabbit, MBL amalgam PM051M) at 1:250 and anti-p63 (mouse, Santa Cruz Biotechnology 4A4) at 1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... an anti-Zif268/Egr1 rabbit antibody (1:1500 for DAB staining; 1:300 for fluorescence staining; C-19, Santa Cruz Biotechnology, Dallas, USA), and anti-mCherry animal antibody (1:1000 for fluorescence staining ...
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bioRxiv - Cancer Biology 2020Quote: ... The blots were washed 3 times in TBS with 0.1% Tween-20 before being incubated with either rabbit (Santa Cruz Biotechnology, SC-2004) or mouse (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2019Quote: ... in PBS for 20 min at room temperature (RT) and incubated with rabbit polyclonal antibodies against ATF3 (Santa Cruz, 1:500, RRID: AB_1078233), and Horseradish peroxidase (HRP)-conjugated mouse monoclonal antibody against GAPDH (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with HRP-conjugated secondary antibodies: donkey-anti-rabbit 1:5000 (Amersham, #NA934V) or goat-anti-mouse 1:5000 (Santa Cruz, #sc-2031), for 1 hour at room temp ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by an overnight incubation at 4°C with Rabbit-anti-NANOG (1/800, Cell Signalling, #3580S) and Mouse-anti-OCT3/4 (1/250, Santa Cruz, sc-5279) antibodies diluted in BB supplemented with 0.1% Triton-X ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at room temperature in the PBSGT solution with the following primary antibody: rabbit anti-PKCγ (1/100, Santa Cruz, sc-211), guinea-pig anti-VGLUT1 (1/3000 ...
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bioRxiv - Microbiology 2020Quote: ... antibody (1:200 in 0.5% FBS-PBS) and secondary goat anti-rabbit IgG-FITC (1:100 in 0.5% FBS-PBS) (Santa Cruz, Cat. No. sc-2356). Spn were visualized on an Axiovert 40 CFL microscope with an Axiocam IC digital camera (Zeiss) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight in rabbit anti-FosB/ΔFosB primary antibody (1:300 in blocking solution, H75, Santa Cruz Biotechnologies, Santa Cruz, CA). The antibody was raised against amino acids 75-100 of human FosB and detects two bands by western blot ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight in rabbit anti-FosB/ΔFosB primary antibody (1:300 in blocking solution, H75, Santa Cruz Biotechnologies, Santa Cruz, CA). The antibody was raised against amino acids 75-100 of human FosB and detects two bands by western blot ...
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bioRxiv - Cell Biology 2020Quote: ... fixed cells were double immunolabeled with rabbit polyclonal anti-K17 (McGowan et al 1998) and mouse anti-K17 (Santa Cruz REF# sc-393002) prior to incubation with PLA probes (Sigma-Aldrich REF# DUO92002 and DUO92004 ...
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bioRxiv - Neuroscience 2019Quote: ... membranes were washed three times with 1X TBST and incubated with horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibodies (1:10,000, Santa Cruz Biotech, Dallas, TX) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... were washed in 500 μl tris buffered saline+0.05% NP40 wash buffer containing 8 μl ZNF804A antibody or 8 μl control Rabbit immunoglobulin (IgG) (Santa Cruz Biotechnology, sc-2027). Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The following secondary antibodies conjugated to horseradish peroxidase were used for western blot detection: Anti-rabbit IgG (Santa Cruz Biotechnology, Cat# sc-2004) and anti-mouse IgG (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were incubated with goat anti-rabbit IgG phycoerythrin-R (PE) conjugated antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were incubated with goat anti-rabbit IgG phycoerythrin-R (PE) conjugated antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used were: mouse anti-PDF C7-s (1:50; Developmental Studies Hybridoma Bank) and rabbit anti-Per1 (1:200; Santa Cruz, CA, USA). Secondary antibodies were ...
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bioRxiv - Pathology 2021Quote: ... we performed immunohistochemistry of MUC5B for human tracheal serial sections with the antiserum (M-25, 1:1000) and previously established commercial rabbit anti-MUC5B antibody (H-300, Santa Cruz, 1:1000). Staining with these antibody and antiserum appeared identical ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with TBS and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:5000, Santa Cruz Biotechnologies, Dallas, TX, EUA) for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... samples had unspecific sites blocked for 1h in a 5% fetal bovine serum albumin solution of washing buffer to be next incubated overnight at 23°C with a polyclonal rabbit anti c-Fos antibody (1:1000 washing buffer, Santa Cruz Biotechnology, USA). After successive washing ...
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bioRxiv - Microbiology 2020Quote: ... stain accessible Fn one colonic tissue fragment of each mice was incubated with 1:200 of rabbit anti-fibronectin (SC9068, Santa Cruz Biotechnologies, USA), or to ii ...
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bioRxiv - Microbiology 2020Quote: ... stain accessible Vn one colonic tissue fragment of each mice was incubated with 1:200 of rabbit anti-vitronectin (SC15332, Santa Cruz Biotechnologies, USA), and finally iii ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblotting was then continued as described above for mouse lung homogenates with mouse monoclonal anti-MUC5AC (Thermo Fischer Scientific #MA5-12178) and rabbit polyclonal anti-MUC5B (Santa Cruz SC-20119) (1:500 vol;vol ...