Labshake search
Citations for Santa Cruz :
2151 - 2200 of 2406 citations for Recombinant Rabbit CCL5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Blots were blocked with 3% non-fat milk-0.05% Tween-20 in Tris buffer saline (TTBS) and incubated with primary antibody anti-GFP (rabbit polyclonal, 1:1000 Santa Cruz Biotechnologies). After wash-out ...
-
bioRxiv - Immunology 2020Quote: ... they were incubated with a 1% BSA solution in 1X PBS containing primary antibody (rabbit polyclonal IgG against human LC3, 1:1000 dilution, Santa Cruz Biotechnology) for one hour at 37 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... or acH4 which recognizes acetylated Lys5, Lys8, Lys12 and Lys16 (Upstate Biotechnology, cat. #06-866) or an equivalent amount of control IgG (anti-rabbit, Santa Cruz, CA) at 4°C overnight ...
-
bioRxiv - Genetics 2022Quote: Nos1ap expression was evaluated in mouse brain cortex lysates using commercially available rabbit polyclonal NOS1AP antibody (R-300, Santa Cruz Biotechnology, TX). Adult mice were euthanized using inhaled isoflurane in a closed chamber ...
-
bioRxiv - Molecular Biology 2022Quote: The following secondary antibodies were used (1/5000) for western blot: horseradish peroxidase (HRP)-conjugated goat antibody against rabbit IgG (sc-2004, Santa Cruz Biotechnology), HRP-conjugated sheep antibody against mouse IgG (NXA931 ...
-
bioRxiv - Physiology 2023Quote: ... Sections were then incubated with primary antibody rabbit anti-UCP1 (1:1000 for WAT and BAT; Santa Cruz Biotechnology INC, Texas, EUA), overnight at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Immunoblotting was performed overnight at 4 °C with the following primary antibodies: anti-calnexin H70 (rabbit polyclonal, Santa Cruz Biotechnology, 1:1000), anti-flotilin-1 clone 18 (mouse monoclonal ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein bands probed with diluted primary antibodies (Table S8) were detected using a goat anti-rabbit or mouse IgG-HRP conjugated secondary antibody (Santa Cruz Biotechnology) and visualized using enhanced chemiluminescence (Amersham Biosciences).
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked in 5% milk-PBST and incubated with 1% milk-PBST containing rabbit anti-Grb7 (C-20, Santa Cruz sc606), anti-β-galactosidase (Invitrogen A11132) ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated overnight at room temperature with rabbit anti-cFOS (1:500; sc-52, Santa Cruz Biotechnology, Santa Cruz, CA) and chicken anti-GFP (1:400 ...
-
bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used at a 1:10,000 dilution: mouse anti-rabbit IgG-HRP (Santa Cruz Biotechnology sc-2357, RRID:AB_628497) and mouse IgGκ BP-HRP (Santa Cruz Biotechnology sc-516102 ...
-
bioRxiv - Molecular Biology 2023Quote: ... ONCs were washed and fixed in 4% paraformaldehyde and incubated with a rabbit polyclonal anti-myc antibody (Santa Cruz Biotech, sc-789) in permeabilizing/blocking solution (0.5% Triton X ...
-
bioRxiv - Microbiology 2023Quote: ... CH58 and CH59 were used as primary antibodies at 1:5000 dilution in PBS and rabbit anti-human Ab HRP conjugate (Santa Cruz Biotechnology) was used as secondary antibodies at 1:10,000 dilution in PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membranes were further incubated at 37°C for 1 hour with horseradish peroxidase-conjugated secondary antibodies: goat anti-rabbit (sc-2004, 1:5000, Santa Cruz Biotechnology) or goat anti-mouse (sc-2005 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated overnight at room temperature with rabbit anti-cFOS (1:500; sc-52, Santa Cruz Biotechnology, Santa Cruz, CA) and chicken anti-GFP (1:400 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies were diluted in 5% milk and used 1:10,000 for anti-rabbit IgG (Amersham, NA934) and 1:15,000 for anti-mouse IgG (goat; Santa Cruz Biotechnology, sc-2064). Chemiluminescence signal was detected using the ECL Select detection kit (GE Healthcare ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4°C with a 1:500 dilution of a Rabbit (Rb) anti-PKCε antibody (sc-214, Santa Cruz Biotechnology) in antibody dilution buffer ...
-
bioRxiv - Neuroscience 2023Quote: Free-floating sections were incubated in rabbit polyclonal Zif268 primary antibody (Egr-1; 1:1000 Cat# sc-110; Santa Cruz Biotechnology, USA) for 72 h at 4°C in PBSTx with 1% NGS ...
-
bioRxiv - Microbiology 2024Quote: ... The membrane was then probed with a 1:5,000 dilution of rabbit anti-goat-HRP 2° antibody (Santa Cruz Biotechnology sc-2768), washed ...
-
bioRxiv - Neuroscience 2024Quote: ... co-staining IBA-1 – ZO-1 incubation during 24 h at room temperature (1:1000, ab178846, rabbit anti-IBA-1, Abcam; 1:200, sc-33725, rat anti-ZO-1, Santa Cruz Biotechnology); and GFAP incubation during 24 h at room temperature (1:1000 ...
-
bioRxiv - Physiology 2023Quote: To assess activation of GnRH neurons dual-label chromogen immunohistochemistry was undertaken with rabbit anti cFos (sc-52, 1:5000, Santa Cruz; RRID:AB_2106783) and biotinylated goat anti-rabbit immunoglobulins (1:400 ...
-
bioRxiv - Neuroscience 2024Quote: ... before staining with the respective primary antibodies (NAc and LH with αGFP chicken 1:1000, Aves Labs ref GFP-1010; αmCherry rabbit, 1:1000, abcam ab167453; and αOrexin goat, 1:500, Santa Cruz Biotech ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated 1h at 25°C in 1% BSA-0.3% Triton X-100-TBS solution and then kept at 4°C in rabbit anti-c-fos antibody (sc-52 LotG1108, Santa Cruz Biotechnology) solution (1:200 in 1% BSA-TBS ...
-
bioRxiv - Immunology 2021Quote: ... The membrane was washed three-times with TBST (TBS containing 0.1% Tween-20) and incubated with HRP-conjugated mouse IgG kappa binding protein (1:5000; Santa Cruz Biotechnology, USA) for 2 hrs at room temperature ...
-
bioRxiv - Biochemistry 2019Quote: ... The following are the specificities and sources of the commercially available antibodies used in the present study: antimyelin basic protein (catalog #sc-13914, Santa Cruz Biotechnology), anti-NG2 proteoglycan (catalog #AB5320 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Antibodies were added for 3 h before 45 min incubation with protein A or G beads (Dynabeads 10002D, 10003D from Thermofisher scientific, or agarose beads sc-2001, sc-2001 from Santa Cruz Biotechnology) for rabbit or mouse IgG respectively ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein, #516102, Santa Cruz), and the HRP activity was detected by incubation with ECL and exposure to a CCD camera ...
-
bioRxiv - Immunology 2021Quote: ... pre-cleared with protein A agarose beads (Pierce) and incubated with agarose conjugated anti-Rad52 mAb (clone F-7; sc-365341 AC, Santa Cruz Biotechnology) at 4°C overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... Presence of His-tagged protein was detected by chemical luminescence using His-probe (H-3) HRP (sc-8036 HRP, Santa Cruz Biotechnology) monoclonal antibody horseradish peroxidase (HRP ...
-
bioRxiv - Microbiology 2021Quote: ... with the lysate overnight at 4 °C followed by addition of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX) and incubation for 2 hours at 4 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18 ...
-
bioRxiv - Microbiology 2019Quote: ... Primary antibody membranes were incubated with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:2500) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:2500 ...
-
bioRxiv - Microbiology 2019Quote: ... Membranes were incubated in TBST containing 2% (w/v) BSA with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:5000) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... were cleared by incubating with 2.0 µg of the control IgG and 20 µL vortexed Protein G PLUS-Agarose beads (Santa Cruz, sc-2002) for 30 min with rocking at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of total protein were separated by SDS-PAGE and probed with primary antibodies against NFATC2 (Santa Cruz Biotechnology, sc-7296), NFATC4 (Cell Signaling ...
-
bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
-
bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
-
bioRxiv - Cell Biology 2022Quote: Cell lysates (500 μg) were pre-cleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz, catalog #: sc-2003) and 1.0 μg of normal mouse IgG antibody (Santa Cruz ...
-
bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
-
bioRxiv - Immunology 2019Quote: ... immunoprecipitated with 2 µg/sample of indicated antibodies overnight at 4 °C before addition of washed protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 1 hr with rotation at 4 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
-
bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 10 μl of a polyclonal ROCK1 antibody (sc-6055, Santa Cruz Biotechnology) coupled to protein G-Sepharose beads (GE healthcare ...
-
bioRxiv - Plant Biology 2019Quote: ... Immunoprecipitated proteins were separated on SDS-PAGE acrylamide gels and western blots were performed using anti-GFP (Santa Cruz Biotechnology sc-9996), anti-SYT1 [7] ...
-
bioRxiv - Plant Biology 2019Quote: ... transferred onto PVDF membrane and proteins were detected using an anti-c-myc monoclonal antibody (sc-40, Santa Cruz Biotech, Texas, USA) and anti-mouse HRP conjugate (W4021 ...
-
bioRxiv - Cancer Biology 2021Quote: ... to detect DARPP-32 (Cat no.: sc-135877; Dilution 1:200) and α-Tubulin (Cat no.: sc-5286; Dilution 1:1000) protein were obtained from Santa Cruz Biotechnology ...
-
bioRxiv - Cancer Biology 2021Quote: ... protein lysates (500 μL of NP-40 buffer containing 200 μg of protein) were incubated with 10 μg of primary antibody Neu (F-11) agarose conjugate (sc-7301; Santa Cruz Biotechnology) overnight at 4°C with gentle rotation ...
-
bioRxiv - Immunology 2020Quote: ... CBLB and GAPDH in the protein blots were probed with anti-CBLB- and anti-GAPDH-antibodies (SC-8006 and SC-166545, respectively; Santa Cruz Biotechnology). Flow Cytometry analysis ...
-
bioRxiv - Cell Biology 2020Quote: ... Supernatants were pre-cleared with control IgG for 1 hr at 4 °C and Protein A/G Plus-Agarose beads (Santa Cruz Biotechnology) for 30 min at 4 °C before incubation with primary antibody overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cbl was immunoprecipitated from 20-50 mg of total protein from each treatment using anti-Cbl AB coupled to agarose beads (sc-170 AC, Santa Cruz Biotechnology). The immunoprecipitated proteins were eluted from Cbl AB-agarose beads with Pierce IgG Elution Buffer (21004 ...
-
bioRxiv - Immunology 2022Quote: ... WCL were incubated with GLUT3 antibody overnight and then with Protein A/G PLUS-Agarose bead slurry (Santa Cruz Biotechnology, sc-2003) for 3h ...