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Citations for Santa Cruz :
1451 - 1489 of 1489 citations for Testosterone D5 98% 100 Ug Ml In Dioxane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 1 μg/ml of Mouse monoclonal H3T3ph antibody 16B2 (Kelly et al., 2010) and 1 μg/ml of mouse monoclonal PCNA antibody (Santa Cruz SC-56) were used ...
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bioRxiv - Genomics 2021Quote: ... The media on human RPTEC was then changed to the fresh lentiviral supernatants supplemented with polybrene (0.5 μg/ml, Santa Cruz Biotechnology; sc-134220) and cultured for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... plates were flooded with detection solution containing 0.1 mg mL−1 2-naphthyl sulfate (potassium salt; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) as a chromogenic substrate coupled with 1 mg mL−1 tetrazotized-o-dianisidine chloride (Fast Blue B salt ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells were seeded in 6-well plates and virus-containing supernatant was added to cells together with Polybrene (10 mg/mL) (Santa Cruz Biotechnology Inc). Cells were then spin-infected for 100 minutes at 2000 rpm at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... and UV-sterilized for 1 h and coated with 0.05 mg/mL rat tail collagen I (sc-136157, Santa Cruz Biotechnology, Dallas, TX) at 4°C overnight.
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bioRxiv - Immunology 2023Quote: ... After an overnight cells were centrifuged in 400g and re-suspended in OKT3-retroviral in the presence of 8μg/ml Polybrene (Santa Cruz Biotechnology, sc-134220). After 12 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by fluorescent staining with Pan-Cytokeratin (PanCK, clone AE-1/AE-3, AF488, Santa Cruz NBP2-33200AF488, [2 µg/mL], CA, USA) to identify trophoblasts ...
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bioRxiv - Genomics 2024Quote: ... 200uL of lentivirus was added to 250,000 cells of the appropriate cell line along with 8ug/mL polybrene (Santa Cruz Biotechnology, sc-134220). The media was changed 24 hours after transduction with 1 μg/ml puromycin (Thermo A1113803 ...
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bioRxiv - Bioengineering 2019Quote: ... Viral titer was determined by serial dilution and infection of HUVECs in the presence of 10 µg ml−1 polybrene (Santa Cruz Biotechnology, Dallas, TX). Titers yielding maximal expression without cell death or detectable impact on cell proliferation or morphology were selected for studies.
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged at 12,000 × g for 5 minutes and then incubated with anti-myosin 5A (G-4) antibody (sc-365986, 0.75 μg/ml, mouse monoclonal; Santa Cruz Biotechnology, Dallas, TX, USA) conjugated to PE-Cy7 (ab102903 ...
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bioRxiv - Molecular Biology 2021Quote: 300 million TetR-CBX7 reporter mESCs were infected with a 1:10 dilution of the harvested virus-containing supernatant PlatinumE cell medium for 24 hrs in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). The 300 million cells were divided into 3x 100 million sets (10x 15 cm plates of 10 million cells each ...
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bioRxiv - Cancer Biology 2020Quote: ... The filtered viral supernatant was used immediately to transduce target cells (PDACc/PANC-1 or fibroblasts) with 10 µg/mL polybrene (Santa Cruz Biotechnology, Dallas, Texas) or stored at −80°C for use in the future ...
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bioRxiv - Cancer Biology 2021Quote: ... Transduction was conducted in 6 well format on 1 × 105 cells and cells plated in suspension into viral supernatant containing 8 μg/ml Polybrene (Santa Cruz Biotechnology: SC-134220) and incubated overnight (16h) ...
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bioRxiv - Systems Biology 2021Quote: ... lentivirus was recovered from supernatant using an Amicon Ultra Centrifugal 50kDa filter (MilliporeSigma) and transduced into HeLa cells along with 10 μg/ml polybrene (Santa Cruz Biotechnology, Dallas, TX). Cells were transduced sequentially with PKAR-FKBP-FP followed by Lyn-FRB ...
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bioRxiv - Molecular Biology 2022Quote: ... was used for generation of the A375-IVLuc cell line by antibiotic selection (Hygromycin b, 200 μg/ml, Santa Cruz Biotechnology, 31282-04-9) and the limiting dilution method for clonal lines generation ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 × 106 Ba/F3 were re-suspended in the viral media and supplemented with 10 μg/mL polybrene (Santa Cruz Biotechnology, Dallas, TX, USA). Transduced cells were selected using 2 μg/mL puromycin (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Gut organoids were treated with increasing concentrations (15 and 30 µg/ml) of IFITMs derived peptides (mouse IFITM2 antibody blocking peptide Santa Cruz sc-373676 P) and blocking antibodies (α-ACE2 AK (AC18Z) ...
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bioRxiv - Cancer Biology 2019Quote: ... Approximately 300 μg of cell lysate at 1-1.5 mg/ml was then pre-cleaned by rotation incubation with 20 μl 50% Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Texas, USA) at 4°C for 60 min ...
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bioRxiv - Cancer Biology 2019Quote: A total of 3-4 x 105 microglia were plated in a 6-well plate and treated with 10 μg/ml immunoglobulin IgG1 control antibody (Santa Cruz Biotechnology, sc-2025) or JAM-A-blocking antibody J10.4 (Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2021Quote: ... Oct4 dual reporter cells were then transduced with the virus for 48 h in the presence of 8 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). Transduced cells with desired TetR-3XFLAG fusion constructs were isolated by FACS sorting for mCherry and western blot confirmation ...
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bioRxiv - Genetics 2021Quote: ... 300 million #H5 clone mESCs were infected with a 1:10 dilution of virus-containing supernatant for 24 h in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220) (Before infection ...
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bioRxiv - Immunology 2020Quote: ... HEK-293 cells expressing either mEmerald-Dectin-1A or pUNO-hDectin-1A were selected using Geneticin (G418 Sulfate) (Thermo-Fischer, #10131035) at 400 μg/ml or Blasticidin (Santa Cruz Biotechnology, #SC-495389) at 20 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Spinal cord tissue was homogenized by mechanical trituration followed by sonication in radio-immunoprecipitation assay (RIPA) lysis buffer (1 ml; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and the cytokine concentration were measured using mouse ELISA kits (Invitrogen Life Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed from dishes and assessed by flow cytometry for construct expression and selected in puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B). Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with PB-vector constructs as above ...
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bioRxiv - Cell Biology 2022Quote: ... BJAB ERAAP-dsRed cells were seeded at 1X10^6 cells/well in a 12-well plate and spin-infected with Cas9 expressing lentivirus in presence of 4ug/mL polybrene (Santa Cruz Biotechnology, sc-134220) by centrifugation at 1,000 g for 2 h at 33 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Target cells were transduced in suspension with the filtered virus-containing media in the presence of 8 μg/mL polybrene (Santa Cruz Biotechnology, #sc-134220).
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bioRxiv - Immunology 2023Quote: ... The complex was then subjected to surface lysine methylation by the addition of 20 µL per mL of complex of 1 M dimethylamine borane complex (ABC) (Santa Cruz Biotechnology, sc-252506) and 40 µL per mL of complex of 1 M formaldehyde prepared from 37 % stock (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Neuroscience 2019Quote: ... Remaining unlabelled primary antibody-bound surface HA-Nrxn1α was blocked with overnight incubation with Fab fragments anti-mouse [0.13 mg/mL (Santa Cruz Biotechnology, Cat #715-007-003) in 5% (wt/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were diluted 1:5 in RIPA buffer and incubated overnight at 4°C with 1 µg/ml myogenin antibody (Santa Cruz Biotechnology, #sc-12732 X) or IgG1 isotype control (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... in complete media in the presence of polarization cytokines and transduced at 24h after activation by spin transduction with polybrene (Santa Cruz Biotechnology, 8 μg/ml) at 2000 rpm ...
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated with 1~10 μg/ml (according to the manufacturer manual) primary antibodies towards proteins of interest (PCNA (Santa Cruz, mouse monoclonal, sc-56), H3K27ac (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... as a chromogenic substrate coupled with 1 mg mL−1 tetrazotized-o-dianisidine chloride (Fast Blue B salt, Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Following 1 h incubation ...
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bioRxiv - Physiology 2023Quote: ... EUA), MitotrackerTM Red CMXRos [15nM] for WAT and [1nM] for BAT (Termofisher Scientific, Massachusetts, EUA) and DAPI (1ug/ml; Santa Cruz Biotechnology INC, Texas, EUA) for 90min ...
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bioRxiv - Cancer Biology 2023Quote: ... The LINE-1 ORF1 shRNA containing lentivirus medium was added to cells with 8 µg ml-1 of polybrene (Santa Cruz Biotechnology, cat# sc-134220). Approximately 48 hrs after infection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently incubated with primary antibodies against Pax7 (Developmental Studies Hybridoma Bank, 2 μg/mL) and MyoD (Santa Cruz Biotechnology, clone G-1, 1:200) diluted in 2.5% BSA and 2.5% GS in PBST ...
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bioRxiv - Pathology 2023Quote: ... all membranes were stripped and re-probed for β-actin using anti-β-actin antibody at a concentration of 0.5 μg/ml (sc-47778, Santa Cruz Biotechnology Inc., Dallas, TX, USA). We used the inbuilt software of the C-Digit Blot Scanner for quantification.
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bioRxiv - Cancer Biology 2023Quote: ... and then frozen at -80°C for later use or applied directly to cells with 4-10 μg/mL polybrene (Santa Cruz Biotechnology, Inc., cat no. SC-134220). The culture media on target plates was changed 24 hours post- transduction.