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Citations for Santa Cruz :
1401 - 1450 of 1489 citations for Testosterone D5 98% 100 Ug Ml In Dioxane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and were incubated with 5 µl of concentrated virus per well and 8 µg/mL polybrene (Santa Cruz Biotechnology, sc-134220). Plates were spun for 5 min at 3200g ...
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bioRxiv - Cell Biology 2019Quote: ... Respective cells were incubated with anti-c-Myc antibody (9E10, mouse monoclonal, 2 mg/ml; Santa Cruz Biotechnology, Dallas, TX, USA) for 1 h at room temperature ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... The next day cells were washed with serum free medium and placed in DMEM supplemented with 2mM L-glutamine with 100µg/ml Penicillin/Streptomycin and 5% v/v FBS or 5% v/v LDS and 1uM compactin (#sc-200853, Santa Cruz). After 48 hours of incubation cells were starved for 4 hours in serum free medium ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were then incubated overnight at 4°C with in house generated primary antibodies against various epitopes of BIN1 at final concentration of 2ug/ml or anti-BIN1 Ab 1:500 (99D and H100, Santa Cruz), respectively ...
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bioRxiv - Pathology 2020Quote: ... Transfected cells were selected by selection media containing 2 μg/ml puromycin dihydrochloride (cat. no. sc-108071; Santa Cruz Biotechnology, Inc.). After confirming the decrease in LOXL2 expression by reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Pathology 2020Quote: ... HK-2 cells were treated with media containing 5 μg/ml of polybrene (cat. no. sc-134220; Santa Cruz Biotechnology, Inc.), and then LOXL2 shRNA lentiviral particles and control shRNA lentiviral particles of 1 and 2 multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2019Quote: ... LTC4 synthesis was initiated by addition of TNP-conjugated BSA (TNP-BSA, 25 ng/mL in DPBS/BSA, Santa Cruz Biotechnology). (N-{(2S,4R)-4-(Biphenyl-2-ylmethyl-isobutyl-amino)-1-[2-(2,4-difluorobenzoyl)-benzoyl]-pyrrolidin-2-ylmethyl}8-3-(4-(2,4-dioxothiazoldin-5-ylidenemethyl)-phenyl]acrylamide (cPLA2 inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... After a further centrifuge red blood cells were lysed in 1 ml of RBC lysis buffer (Santa Cruz Biotechnology sc-296258) in ice for 2.5 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were transduced by spin infection at 1200 × g at 32 °C for 90 min in the presence of 10 μg/ml polybrene (Santa Cruz) 12–16 h post-activation with anti-CD3/anti-CD28 ...
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bioRxiv - Biophysics 2022Quote: Transcription induction of the transfected construct was done 24 h after transfection using 2 μg/mL doxycycline (Santa Cruz # sc-204734B) in Tet-free medium ...
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bioRxiv - Genomics 2022Quote: ... K562 was transduced by spinfection at 2,000g for 90 min at 37 °C with 8 µg/ml polybrene (Santa Cruz Biotechnology). To generate the M.CviPI overexpression bulk cell lines ...
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bioRxiv - Neuroscience 2022Quote: ... Spinal cord tissue was homogenized by mechanical trituration followed by sonication in radio-immunoprecipitation assay (RIPA) lysis buffer (1 ml; Santa Cruz Biotechnology ...
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bioRxiv - Systems Biology 2024Quote: ... Reporter cells were then transduced with the virus in the presence of 8 µg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). After 7 days of transduction ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were first incubated overnight at 4°C and 300rpm with p21WAF1/CIP1 (Santa Cruz, SC-817, 1:200, 1.0µg/mL), suspended in a 2.5% NGS solution ...
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bioRxiv - Cancer Biology 2024Quote: ... lentivirus were mixed in a ratio 1:1 with fresh complete culture medium and supplemented with 10 μg/mL polybrene (Santa Cruz) to transduce them ...
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bioRxiv - Genomics 2023Quote: ... then incubated with 1 μg/mL of primary antibodies specific to phosphorylated (Ser 139) H2AX (cat. # sc-517348, Santa Cruz Biotechnology) in 3% BSA overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-PAX7 antibody at 5 µg/mL (DSHB, Iowa, U.S.A.) and Rabbit anti-MyoD antibody at 1:500 (sc-760, Santa Cruz, Texas, U.S.A.).
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bioRxiv - Biophysics 2023Quote: ... and then incubated overnight at 4°C with the following primary antibodies: anti-Laminin β1 (4μg/ml, sc-17810, Santa Cruz Biotech), anti-Fibronectin (10 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The pellet was rinsed with PBS and suspended in150 mM Tris-HCl buffer (pH 7.2) of the same volume as the RIPA buffer containing Benzonase® nuclease (250 U/mL, Santa Cruz). The sample was incubated at 25 °C for 2 h with intermittent mixing (Thermomixer comfort ...
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bioRxiv - Cancer Biology 2023Quote: ... Target cells were plated 24 hours before infection and then exposed to viral supernatant supplemented with 20 μg/ml polybrene (Santa Cruz). Mouse cell lines were then selected for at least 72 hours with 4 μg/ml puromycin (Corning) ...
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bioRxiv - Biochemistry 2023Quote: ... K562s were transduced with the appropriate lentivirus by spinfection (1,800g, 90 min, 37 °C) with 12 μg ml−1 polybrene (Santa Cruz Biotechnology). 2 μg ml−1 puromycin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The following assay positive controls were used at a starting concentration of 1 µg/ml: (1) Anti-ASC/TMS1/PYCARD Antibody (B-3) mouse monoclonal IgG1 (sc-514414, Santa Cruz); (2 ...
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bioRxiv - Cancer Biology 2024Quote: ... efficiently transduced cells were selected by two rounds of 5-day treatment with 2.5 µg/ml puromycin (sc-108071A, Santa Cruz Biotechnology). Individual cell clones were isolated by limiting dilution seeding in 96-well plates and exposed to 2.5 µg/ml puromycin every 6th passage.
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with p53 antibody Alexa Fluor® 546 (Santa Cruz, sc-99 AF546, 1:50 dilution, 4 μg/ml) in dark for one hour in room temperature ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with p53 antibody Alexa Fluor® 647 (Santa Cruz, sc-126 AF647, 1:50 dilution, 4 μg/ml) in dark overnight in 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were serum-starved overnight and the following day they were pre-incubated with 1 µg/mL mitomycin C (CAS 50-07-7, sc-3514A; Santa Cruz Biotechnologies) 1 h prior to the scratch generation to block proliferation ...
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bioRxiv - Cancer Biology 2020Quote: ... Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride, sc-108071B, Santa Cruz Biotechnology) and after the second transduction also 125 µg/mL hygromycin (Hygromycin B ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation was performed by incubating samples in 2 g/mL anti-rabbit (FL-393, sc-6243) or anti-mouse (DO-1, sc-126) P53 antibodies (Santa Cruz Biotechnology) for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and 24 hours post-seeding they were transduced using optimal viral titer and 8 μg/ml polybrene (#sc-134220, Santa Cruz Biotechnology). Transduced cells were selected with 1 μg/ml puromycin (#P8833 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% tetracycline-free fetal bovine serum (FBS) and the induction of N-Myc expression was obtained by adding 1 µg/ml doxycycline (tetracycline analogue; Santa Cruz Biotechnology). Treatment with 9 µM Ro-3306 (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2019Quote: ... Dox-treated Gal4-EED/luc cells were transfected in 12-well plates and then grown for 24 hours before the addition of 10 µg/mL puromycin (Santa Cruz Biotechnology) to Gibco DMEM high glucose 1× (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Fractions obtained from the ODG were diluted with 8 ml of PBS containing 1 × EDTA-free protease inhibitor cocktail (Santa Cruz, USA), and concentrated using a 10 kDa spin concentrator to remove the excess Optiprep® solution.
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bioRxiv - Systems Biology 2021Quote: ... cells were incubated in primary antibody solution (3% BSA in PBS-T) with 1 μg/mL of both α-RELA (sc-8008; Santa Cruz) and NEMO (sc-8330 ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... and the cells were transduced with lentivirus (250 μl/cm2) in Macrophage Maturation Medium containing 2 μg/ml Polybrene (Santa Cruz, sc134220) by spinfection (centrifugation at 1000 x g for 1 h at 32°C) ...
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bioRxiv - Physiology 2021Quote: ... adenovirus was added to the medium for 3 hours on day 4 of differentiation at 250 MOI in the presence of 5 μg/ml polybrene (Santa Cruz Biotechnology). Experiments were performed 3 days later ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were then treated for 1h with 25 μg/mL of Caffeic Acid Phenethyl Ester inhibitor (CAPE, Santa Cruz Biotechnology, sc-200800) or DMSO ...
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bioRxiv - Neuroscience 2022Quote: The sections were then washed with PBS and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Santa Cruz Biotechnology) to visualize nuclei ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in 500 µL of cold PBS 1X and fixed by adding 4.5 mL of 4 % paraformaldehyde in saline (sc-281692, Santa Cruz Biotechnology, France) and incubating at room temperature for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus-containing media was centrifuged and added to MDA-MB-468 cells for shRNA transduction with Polybrene (8 μg/mL, # sc-134220A, Santa cruz biotechnology). Then ...
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bioRxiv - Genomics 2024Quote: ... lentiviral transduction was performed by mixing cells in media supplemented with 8 µg/mL of polybrene (Santa Cruz Biotechnology 28728-55-4) with lentivirus and centrifuging in cell culture plates at 1000 xg and 33 °C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... 80% confluent RHMVECs in 6-well plates were transduced with human T111I EL (pKS18) lentiviruses with 4 μg/ml Polybrene (#134220; Santa Cruz Biotechnology) in a total volume of 1 ml DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology). Transduced cell lines were selected in puromycin and/or blasticidin (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... HCT116 EGFP-SON clone C4 was transduced with a lentivirus carrying the Lenti-mCherry-PCNA construct (Xiong et al., 2023) by supplementing media with 8 μg/ml polybrene (Santa Cruz Biotechnology).
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 cells were transfected with pcDNA3.1-YAP1 (G418 resistant) or empty vector as previously described and treated with 500 μg/ml G418 (Santa Cruz, Cat. #sc-29065) for 4 weeks to obtain stable cell lines ...
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bioRxiv - Immunology 2021Quote: ... The cells were resuspended in the lentivirus-containing medium with addition of 8 μg/ml polybrene (Santa Cruz Biotechnology, Cat. no: sc-134220) and centrifuged for 90 min at 2,500 g at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...
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bioRxiv - Molecular Biology 2021Quote: ... The culture media was filtered through a 0.45-μm filter and added to A549 cells along with Polybrene (4 μg/ml; Santa Cruz Biotechnology, Dallas, TX). At 72 h after transduction ...
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bioRxiv - Microbiology 2020Quote: ... 1 ml of the bacterial suspensions (108 cells mL−1 was added under constant agitation, thus obtaining the mixed inoculants. Tomato (Santa Cruz, giant Kada) and papaya (Caliman-01 - UENF ...
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bioRxiv - Genomics 2022Quote: ... The media on human RPTEC was then changed to the fresh lentiviral supernatants supplemented with polybrene (0.5 µg/ml, Santa Cruz Biotechnology; sc-134220) and cultured for 24 h ...
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...