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Citations for Santa Cruz :
1051 - 1099 of 1099 citations for Recombinant Human UGT1A1 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Nucleus proteins of the ANKRD49-OE or LV5 H1299 cells (1×106) were extracted and 25 µg of them were precleared using Protein A/G Agarose (Santa Cruz, California, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates (500 µg) were pre-cleared with 30 µl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate PARP14 by incubating with 2 µg of a mouse monoclonal antibody against PARP14 (Santa Cruz, sc-377150), and protein G agarose beads overnight at 4°C with gentle mixing on a nutator at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of protein were first separated by SDS-PAGE and then blotted with the following antibodies: MSH3 (1:500, Santa Cruz, sc-271080), huntingtin (1:2,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining supernatant was subjected to immunoprecipitation using 15 μl of mouse monoclonal antibody against green fluorescent protein (catalog no. sc-9996, Santa Cruz, CA, USA). The immunoprecipitate was gently shaken overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: LDLR cell surface protein was quantified by fixing cells in 4% paraformaldehyde and incubated with anti-LDLR at 1:100 (Santa Cruz Biotechnology, sc18823) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Separated proteins were transferred to a PVDF membrane and the membrane was incubated with antibodies to the HA epitope tag (Santa Cruz Biotechnology, sc-805) and α tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with the antibodies for 1h at 4°C on a roller before 80µl Protein G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA) were added and samples were incubated overnight at 4°C on a roller ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour on a rotator at 4°C and then incubated with 50 μL protein A/G PLUS-Agarose (#sc-2003, Santa Cruz Biotechnology, Dallas, Texas) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Approximately 300 μg of cell lysate at 1-1.5 mg/ml was then pre-cleaned by rotation incubation with 20 μl 50% Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Texas, USA) at 4°C for 60 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Blots were then washed three times for 15 minutes at 4 °C with 0.05% TBS-Tween and stained for 4 hours with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP) (Santa Cruz Biotech, sc-516102) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4, SC-47778, mouse monoclonal antibody, Santa Cruz Biotechnology, USA), anti-GFP (B-2 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Molecular Biology 2022Quote: ... The remained liquids were added with anti-NS1 or -VP2 or -IgG antibodies and incubated at 4°C for 4 h followed by addition of protein A/G PLUS-Agarose (Santa Cruz Biotech, sc-2003) and incubation overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to each sample and then incubated at 4°C for 4 hours followed by addition of protein A/G PLUS -Agarose (Santa Cruz Biotech, sc-2003) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins extracts (1.5 mg) of embryos expressing CG11412-myc were incubated with 1 μg of c-Myc antibody (Santa Cruz, sc-40, clone 9E10) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µg of protein extract was incubated at 37°C for 1 hr with the artificial p-nitrocatecholsulfate substrate (Santa Cruz Biotechnology, sc-238927A) and either sodium pyrophosphate (ARSA ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibodies to cellular proteins were used as markers for early endosomes (EEA-1; rabbit polyclonal antibody; Santa Cruz Biotechnology Inc; 1:200), and the trans-Golgi (TGN46 ...
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bioRxiv - Cell Biology 2023Quote: ... were immunoprecipitated by incubation with protein A/G magnetic beads (HY-K0202, MCE) and anti-GFP antibody (sc-9996, Santa Cruz Biotechnology; 1:50) at 4 °C overnight with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were loaded per lane and blots were probed with anti-mGFP antibodies (Santa Cruz Biotech, #sc-9996; 1:2000 dilution), anti-elongation factor 1-α (EF1A ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated with primary antibody or rabbit IgG (control) for 3hr at 4°C and then with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) beads for 35min with head-to-tail rotation either in the absence or presence of 100 µM CCT blocking peptide SAGRRFIVSPVPE (United Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: Protein expression was quantified by western blot analysis using the following antibodies: anti-cyclin A (1:500 sc-751; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Histone H3 (phospho Serine10 ...
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bioRxiv - Cell Biology 2022Quote: ... or mouse brain homogenates (1 mg) were pre-cleared with 30 µL of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Zoology 2019Quote: Protein was extracted from 5 live larvae using the methanol/chloroform protocol [33] and solubilized in electrophoresis sample buffer (Santa Cruz Biotechnology, Dallas, TX, USA) for SDS-polyacrylamide gel electrophoresis and Western Blot analysis ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... 500-1000ug protein extracts were incubated under rotary agitation overnight at 4 °C with antibodies against SMARCA4 (Santa Cruz Biotechnology, sc-374197 X; 4 µg), YAP (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated with 1~10 μg/ml (according to the manufacturer manual) primary antibodies towards proteins of interest (PCNA (Santa Cruz, mouse monoclonal, sc-56), H3K27ac (Millipore ...
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bioRxiv - Neuroscience 2019Quote: Coimmunoprecipitation was performed as previously described (Waxman, Baconguis, Lynch, & Robinson, 2007) with the following substitutions: Protein A/G agarose (Santa Cruz Biotechnology, Santa Cruz, CA) was used to bind 3ug of antibody ...
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bioRxiv - Molecular Biology 2020Quote: Cellular protein was collected with a low-salt lysis buffer and was subjected to immunoblotting analysis with GAPDH (1:4000, Santa Cruz Biotechnology Inc., CA, USA) or iNOS (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Developmental Biology 2023Quote: ... detection was carried out with the mouse IgG kappa binding protein conjugated to horseradish peroxidase (1/100) (sc-516102, Santa Cruz Biotechnology, Dallas, TX, USA) and the TSA Plus Cyanine 3 Kit (NEL744001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... and the resulting pellet was evaluated for the presence of specific protein marker of EVs (endosome pathway marker): ALIX (1:750, sc49267, Santa Cruz Biotechnology, Dallas, TX, USA). As negative marker was used the proteins GRP78 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to PVDF membrane (Amersham 0.2 Hybond P, Cytiva) and revealed using following antibodies: mouse AGR2 (Santa Cruz Biotechnology, Clone 6C5, 1/1000), rabbit IRE1β (gift from David Ron ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% milk/TBST for 30 min and incubated with primary antibodies/TBST against proteins of interest: anti-RBM12 (Santa Cruz Biotechnology, Cat. #sc-514258), anti-PKA alpha polyclonal antibody (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used to probe GST or epitopes (V5, Flag, or HA) for HSV-1 proteins include mouse monoclonal GST antibody (Santa Cruz Biotechnology Cat#: sc-138) diluted at 1:1,000 ...
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bioRxiv - Genomics 2023Quote: ... 200 µL of chromatin (from approximately 7×106 MCF10A cells) were pre-cleared per condition by incubating with Protein A/G beads (Santa Cruz #SC-2003. Lot#B2323) for two hours at 4°C on a rotator ...
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bioRxiv - Cell Biology 2024Quote: ... Tumour lysates were subjected to SDS-PAGE followed by protein transfer and immunoblotting with primary antibodies (anti EPLIN, Novus Cat NB100-2305 and anti β-actin, Santa Cruz Cat sc-47778). Signal amplification was performed with HRP-conjugated secondary antibodies (Goat Anti Mouse IgG and Anti Rabbit IgG von Southern Biotek ...
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bioRxiv - Immunology 2024Quote: ... Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3, VDR, Gfi1) using protein G agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA). Eluted conjugates were run on SDS-PAGE and probed with primary antibodies of partner proteins ...
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... and then a 1:5000 (v/v) solution of horse-radish peroxidase (HRP) conjugated mouse IgG kappa binding protein (Santa Cruz Biotechnology Cat. No. sc-516102) in 1% BSA solution in 1X TBST buffer was incubated with the membrane at room temperature for one hour ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Microbiology 2022Quote: ... then incubated with HRP-conjugated secondary mouse anti-rabbit IgG or mouse IgG kappa binding protein antibodies (1:10,000, Santa Cruz Biotechnology sc-2357 and sc-516102) at RT for 1 h ...
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bioRxiv - Neuroscience 2019Quote: EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328, Santa Cruz Biotechnology; ratio: 1:1) for 2 h at room temperature prior to the addition to the cells(26).