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Citations for Santa Cruz :
951 - 1000 of 1099 citations for Recombinant Human UGT1A1 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed under conditions in which only covalent interactions were preserved with anti-GFP (mouse) antibody overnight at 4°C following which Protein A agarose beads (Santa Cruz) were used to precipitate the antibody bound complex.
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bioRxiv - Cell Biology 2020Quote: Total protein from cultured cells was homogenized in RIPA lysis buffer with a mixture of protease and phosphatase inhibitors (Santa Cruz). Protein concentration was determined using the Bradford assay (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... One mg of total protein of each sample were immunoprecipitated with 50 ul of p53 antibody (D0-1, sc-126, Santa Cruz) and incubated overnight on rotating wheel at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed and preincubated with Protein A/B PLUS agarose beads (Cat# sc-2003, Santa Cruz Biotech, Dallas, TX, USA) for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the remaining protein fraction and kept at 4 °C for 2 hrs on a rotating apparatus followed by overnight incubation with 30 μl of the protein G plus agarose beads (#SC-2002, Santa Cruz) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The following HRP-conjugated secondary antibodies or proteins were used for development of western blots at 1:5000 – 1:10000: mouse IgGκ-binding protein (m-IgGκ BP-HRP) (sc-516102, Santa Cruz); mouse monoclonal anti-rabbit IgG-HRP (sc-2357 ...
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bioRxiv - Immunology 2021Quote: ... One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz) and incubating for 1 hour at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the detection of CDK4 proteins via a quick WB procedure using the sc-601 primary antibody from Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... was from Sigma and the HRP-conjugated anti-mouse IgG-k-chain binding protein (sc-51602) as well as the anti-goat HRP-conjugated secondary antibody (sc-2350) were from Santa Cruz Biotechnology.
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bioRxiv - Plant Biology 2019Quote: ... Expression of bait fusion proteins was confirmed by immunoblotting with anti-c-Myc (clone 9E10, Santa Cruz Biotechnologies, Dallas, TX, USA) and goat anti-mouse IgG-HRP conjugate (Jackson Immuno Research ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and then the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). Diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] reagent ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was pre-cleared by O/N incubation with 40 μL of Protein A/G beads (sc-2003, Santa Cruz biotechnology) on a stirring wheel at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein of cleared lysate were incubated with anti-GFP polysera (1:1,000) overnight prior to addition of protein-G agarose beads (Santa Cruz Biotechnology) for an additional 2 hr ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were prepared from microdissection of 10 hippocampi of juvenile mice incubated in RIPA lysis buffer (Santa Cruz sc-24948). Active Rac1 Detection was performed using Active Rac1 Detection Kit of Cell Signalling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... INSR was immunoprecipitated by incubating 500 μg of protein lysate with 2 μg INSR antibodies (Rabbit pAb, Cat. #sc-711, Santa Cruz) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Different primary antibodies were used for the detection of interest proteins: anti-IL-1β rabbit polyclonal (1:1000, H-153, SC-7884, Santa Cruz), anti-caspase-1 (p20 ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently incubated with primary antibodies rabbit anti-Prosurfactant Protein C (pro-SPC) (MilliporeSigma, Burlington, MA, USA, #AB3786, RRID: AB_91588) and mouse anti-Acetylated α Tubulin (ACT) (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... For co-localization studies employing confocal microscopy the following antibody combinations were used: mouse anti-non-structural protein 1 (Santa Cruz)/goat anti-mouse Alexa568 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were stripped and re-probed with a polyclonal antibody directed against the immunoprecipitated GRK2 protein (C15, Santa Cruz Biotechnology Inc). Blots were developed using a chemiluminescent method (ECL ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl DynabeadsTM Protein G beads were first coated with 1 µg mouse anti-MYC antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG1 (sc-3877 ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting immune complex was then incubated for 1 h with 40 µl of protein A/G plus agarose beads (sc-2003, Santa Cruz). After this ...
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bioRxiv - Cell Biology 2023Quote: ... 4 °C) and then pre-cleared with 20 μl of protein A/G PLUS agarose beads (sc-2003, Santa Cruz Biotechnology) for 1 hour at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... supernatant was incubated with anti-NLRP3 or anti-ZDHHC7 antibody as indicated and Protein A/G conjugated agarose beads (Santa Cruz) to immunoprecipitate endogenous NLRP3 or ZDHHC7 proteins ...
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bioRxiv - Neuroscience 2023Quote: ... anti-S6 ribosomal protein (S6) (mouse monoclonal clone C-8, #sc-74459, RRID: AB_1129205, Santa Cruz Biotechnology; 1:1000 for WB), anti-4E-BP1 (rabbit monoclonal clone 53H11 ...
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bioRxiv - Cancer Biology 2024Quote: ... 300 μg nuclear extracts diluted in 500 μL IP lysis buffer was pre-cleared by incubating with 20 μL protein A/G agarose beads (Santa Cruz) for 2 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg envelope plasmid pMDG encoding VSV-G protein and 1.5 µg lentiviral vector plasmid encoding shRNA to MSI-2 (sc-75834-SH; Santa Cruz Biotechnology). A lentiviral vector plasmid encoding scrambled shRNA sequences with no cellular targets was used as the negative control (sc-108060 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000 µg of total protein suspended in 500µL of cell lysate was precleared with 20 µL of protein A/G plus agarose beads (Santa Cruz Biotechnology) and 1 µL of normal rabbit IgG (Cell Signalling ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed three-times with TBST (TBS containing 0.1% Tween-20) and incubated with HRP-conjugated mouse IgG kappa binding protein (1:5000; Santa Cruz Biotechnology, USA) for 2 hrs at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The following are the specificities and sources of the commercially available antibodies used in the present study: antimyelin basic protein (catalog #sc-13914, Santa Cruz Biotechnology), anti-NG2 proteoglycan (catalog #AB5320 ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were added for 3 h before 45 min incubation with protein A or G beads (Dynabeads 10002D, 10003D from Thermofisher scientific, or agarose beads sc-2001, sc-2001 from Santa Cruz Biotechnology) for rabbit or mouse IgG respectively ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein, #516102, Santa Cruz), and the HRP activity was detected by incubation with ECL and exposure to a CCD camera ...
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bioRxiv - Immunology 2021Quote: ... pre-cleared with protein A agarose beads (Pierce) and incubated with agarose conjugated anti-Rad52 mAb (clone F-7; sc-365341 AC, Santa Cruz Biotechnology) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... samples containing same protein amount were subjected to immunoprecipitation with rabbit polyclonal antibody against PDE4B (H-56) (#sc-25812, Santa Cruz Biotechnology) coupled to GammaBind Plus Sepharose beads (# 17-0886-02 ...
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bioRxiv - Microbiology 2021Quote: ... with the lysate overnight at 4 °C followed by addition of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX) and incubation for 2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated overnight with combinations of the following primary antibodies: rabbit anti-protein kinase C (anti-PKC) clone C20 (1:1000; Santa Cruz Biotechnology), rabbit anti-phospho-histone H3 (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibody membranes were incubated with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:2500) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated in TBST containing 2% (w/v) BSA with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:5000) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cleared by incubating with 2.0 µg of the control IgG and 20 µL vortexed Protein G PLUS-Agarose beads (Santa Cruz, sc-2002) for 30 min with rocking at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of total protein were separated by SDS-PAGE and probed with primary antibodies against NFATC2 (Santa Cruz Biotechnology, sc-7296), NFATC4 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates (500 μg) were pre-cleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz, catalog #: sc-2003) and 1.0 μg of normal mouse IgG antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Immunology 2019Quote: ... immunoprecipitated with 2 µg/sample of indicated antibodies overnight at 4 °C before addition of washed protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 1 hr with rotation at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 10 μl of a polyclonal ROCK1 antibody (sc-6055, Santa Cruz Biotechnology) coupled to protein G-Sepharose beads (GE healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoprecipitated proteins were separated on SDS-PAGE acrylamide gels and western blots were performed using anti-GFP (Santa Cruz Biotechnology sc-9996), anti-SYT1 [7] ...