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Citations for Santa Cruz :
651 - 700 of 7661 citations for 7 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... and the cells were transduced with lentivirus (250 μl/cm2) in Macrophage Maturation Medium containing 2 μg/ml Polybrene (Santa Cruz, sc134220) by spinfection (centrifugation at 1000 x g for 1 h at 32°C) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20 ng/ml IL-6 and 4 hours post-treatment lysed using CHAPS buffer and incubated overnight with 2 μg of an anti-ETV7 antibody (TEL2, Santa Cruz Biotechnologies) or normal mouse IgG (Santa Cruz Biotechnologies ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated in blocking buffer (Amersham Biosciences) for 60 minutes at room temperature and in primary antibody (BRF1/2: CST#2119, GR: CST#3660, GAPDH: Santa Cruz sc32233) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ATDC5 and SaOS-2 cells were transfected with the mouse or human SHIP2 double nickase plasmid (Santa Cruz, sc-421138-NIC (mouse), sc-401622-NIC (human) ...
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bioRxiv - Genomics 2023Quote: ... Samples were pre-cleaned by incubating with 2–4 μg of normal immunoglobulin G (IgG) and 50 μl of A / G-agarose protein microspheres (Santa Cruz Biotechnology) for 1 hour at 4 °C with agitation for preclearance ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology). Transduced cell lines were selected in puromycin and/or blasticidin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Alomone Labs) or 5 µM of antibodies for targeting neurons with dopamine receptor 2 (D2R) (anti-D2DR, sc-5303, Santa Cruz Biotechnology) or without cell type specificity (rabbit anti-human IgG ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged to remove debris and the supernatant was rocked at 4°C for 2 h with an anti-Flag antibody (anti-DDDDK tag, MBL Life Science) or mouse anti-IgG antibody (Santa Cruz Biotechnology) that was conjugated with ‘Dynabeads Protein A’ (Veritas ...
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bioRxiv - Genomics 2023Quote: ... The reaction was incubated on ice for 10 min and super-shift assay was performed by adding 2 µL of anti-HuR antibody (3A2, mouse monoclonal IgG antibody, Santa Cruz Biotechnology). After antibody addition ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cell pellets were then incubated with room temperature PBS containing 2 mM DSG (disuccinimidyl glutarate, sc-285455, Santa Cruz Biotechnology) for 25 min with gentle agitation ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was separated by centrifugation at 12.000 g at 4 °C for 10 min and incubated with STAT2 antibody (2 µg) (Santa Cruz Biotechnology, 514193) for 3 h at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitations were performed using 7 μg of rabbit polyclonal anti-Tgif1 antibody (Santa Cruz Biotechnology) or anti-rabbit ChIP grade IgG (Abcam) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Immunology 2021Quote: ... JUND (D-9, sc-271938), SMAD3 (38-Q, sc-101154), MAX (H-2, sc-8011), and MYC (9E10, sc-40) (all, Santa Cruz Biotechnology, USA) followed by peroxidase-conjugated anti-mouse antibodies (NA931 ...
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bioRxiv - Molecular Biology 2020Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...
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bioRxiv - Molecular Biology 2020Quote: ... were washed with 200 μL HBS (150 mM NaCl, 10 mM HEPES, pH7.5 by KOH) and incubated with 2 μg anti-HA (Santa Cruz, Cat.# sc-7392) or 2 μg rabbit IgG isotype (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... all media was removed from the plate and replaced with 8 ml of 2 µg/ml puromycin (Santa Cruz Biotech cat # sc-108071) L-15 selection media ...
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bioRxiv - Cancer Biology 2020Quote: ... Cas9-expressing cell lines were infected at a density of 2×105 cells in 1.5 mL media in the presence of 4 μg/mL polybrene (Santa Cruz Biotechnology, SC-134220). sgRNA resistant human ZRSR2 DNA fragment was synthesized by Twist Bioscience and cloned into pRRL.idTomato.
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 5% skimmed milk powder for 2 hours at room temperature.Incubate primary antibody overnight at 4°C.The membrane was washed three times with TBST and then incubated at room temperature with conjugated HRP secondary antibodies for 2 hours.ECL (enhanced chemiluminescence kit, Santa Cruz Biotechnology, Dallas, TX) detection system (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... RNA Pol II and NF-YA levels were measured using an anti-Rpb3 antibody60 and anti-NF-YA antibody (G-2; Santa Cruz, sc-17753X) respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight rotating at 4 °C together with either α-KAP1 or α-RIF1 antibodies (see Supplementary Table 2) or IgG only control (#sc-2026, Santa Cruz), 10% of chromatin was isolated as input control ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were passaged as needed in the presence of 2 μM Rho-associated kinase (ROCK) inhibitor (RI) thiazovivin (Santa Cruz Biotechnology, #SC-361380). Prior to transfection ...
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bioRxiv - Genetics 2022Quote: ... Once the cells were ∼50% confluent (after 2-3 days) the media was changed to StemFlex with 1ug/uL polybrene (Santa Cruz SC-134220), and 10uL each of Ngn2 and rtTA virus was added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... catalog number sc7392), α-GFP (clone B-2, catalog number sc9996), and α-ubiquitin (clone P4D1, catalog number sc8017) antibodies were purchased from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate PARP14 by incubating with 2 µg of a mouse monoclonal antibody against PARP14 (Santa Cruz, sc-377150), and protein G agarose beads overnight at 4°C with gentle mixing on a nutator at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Monoclonal antibodies against HA-epitope (clone F-7) and Ubiquitin (P4D1) were purchased from Santa Cruz Biotechnology ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The cleared cell lysates are incubated with mouse α-HA (F-7, Santa Cruz, #Sc-7392), or mouse α-GFP (3E6 ...
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bioRxiv - Physiology 2024Quote: ... and incubated overnight with primary antibody against mouse Cx43 (unconjugated F-7, mouse, Santa Cruz Biotechnology) 1/200 in PBS/0.1% Triton™ X-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: 300 million TetR-CBX7 reporter mESCs were infected with a 1:10 dilution of the harvested virus-containing supernatant PlatinumE cell medium for 24 hrs in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). The 300 million cells were divided into 3x 100 million sets (10x 15 cm plates of 10 million cells each ...
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bioRxiv - Biophysics 2020Quote: ... using EZ-Retriever V.2 (BioGenex, San Ramon, CA, USA) and immunostained with anti-α-SMA monoclonal antibody (Cat No.sc-32251, Santa Cruz Biotechnology, CA, USA) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and β5-integrin cells were incubated for 20 min before chase and during pulse with 10 μM gefitinib (Santa Cruz Biotechnology, 184475-35-2), 10 μM PP2 (Thermo-Scientific ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates of equal protein amounts were incubated overnight at 4°C (constant rotation) with 2 μg of an anti-HA antibody or immunoglobulin G (IgG) control (sc2027; Santa Cruz Biotechnology, Dallas, TX). Co-immunoprecipitation of STAT3 with associated proteins was then performed by using Protein A/G Agarose beads included in the Pierce Classic IP Kit (26146 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were blocked in 3% BSA in PBS with 0,2% Tween 20 and incubated overnight with anti-MYC (A-14, Santa Cruz Biotechnology, 2 ug/ml), anti-CENP-C (PD030 ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed from dishes and assessed by flow cytometry for construct expression and selected in puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B). Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with PB-vector constructs as above ...
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bioRxiv - Immunology 2023Quote: ... Lungs were dissected at the stated time post tumor initiation and fixed for 24 hours in 2% paraformaldehyde (Santa Cruz Biotechnology Cat# sc-253236) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunofluorescence were Rat Anti-C-peptide (Developmental Studies Hybridoma Bank, ID-4) and Goat Anti-GLP-2 (Santa Cruz Antibodies, C-20).
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bioRxiv - Biochemistry 2022Quote: ... Anti-Syntaxin 7 (sc-514017) and anti-Vamp8 Alex Fluor 488-conjugate were purchased from Santa Cruz Biotechnology (sc-166820 AF488) ...
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bioRxiv - Molecular Biology 2019Quote: ... Panobinostat (CAS 404950-80-7) and α-amanitin (CAS 23109-05-9) were purchased from Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2022Quote: ... Stu2 was detected using mouse anti-HA (F-7 clone, SC7392, Santa Cruz Biotechnology, Santa Cruz, CA). Secondary antibodies for these blots were goat anti-rabbit conjugated with HRP from Jackson Immuno Research Labs (cat #111-035-144 ...
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bioRxiv - Genetics 2022Quote: ... Stu2 was detected using mouse anti-HA (F-7 clone, SC7392, Santa Cruz Biotechnology, Santa Cruz, CA). Secondary antibodies for these blots were goat anti-rabbit conjugated with HRP from Jackson Immuno Research Labs (cat #111-035-144 ...
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bioRxiv - Genetics 2019Quote: ... the collected precipitates were analyzed by immunoblotting (IB) with antibodies raised against ZP2 (C-7, Santa Cruz), ZP3 (H-300 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse anti-Brg1 (G-7; sc-17796) and normal rabbit IgG (sc-2027) were from Santa Cruz Biotechnologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were incubated with a polyclonal antibody against LASP1 (G-7; sc-374059, Santa Cruz Biotechnology (SCBT)) and immune complexes visualised using Alexa 594 secondary antibodies (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA) and Bcl-2 (mouse monoclonal ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing IgG to the SARS-CoV-2 nucleoprotein (NP) and with mouse anti-human GAPDH monoclonal antibody (G-9: Santa Cruz Biotechnology, Dallas, TX, USA). The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma ...
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bioRxiv - Genomics 2021Quote: ... iPSC-derived MSCs were cultured to at least passage 6 and seeded at 4.2 × 104 cell/cm2 in culture dishes (4-10cm diameter) coated in 5ug/cm2 Type I Collagen (sc-136157, Santa Cruz Biotechnology, Dallas, TX, USA). After culturing cells for 1 day in MSC medium ...