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Citations for Santa Cruz :
5301 - 5350 of 5356 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and a 1:100 dilution of anti-stage-specific embryonic antigen-1 (SSEA-1) mouse IgM antibody (sc-2170; Santa Cruz Biotechnology, Dallas, TX, USA) with 1% blocking reagent TNT for 1 hour on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... The same samples were also immunolabeled with FAK/pFAK or Paxillin antibodies (FAK C-20, 1/200, Santa Cruz, Ref: sc-558. p-FAK(Y397), 1/500 ...
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bioRxiv - Physiology 2024Quote: ... Samples were incubated with primary antibodies (Connexin 43: C6219, Sigma-Aldrich, dilution 1:500; Connexin 37: ARP36603-P050, Aviva, dilution 1:300; Dab2: sc-136964, Santa Cruz, dilution: 1:400) overnight ...
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bioRxiv - Immunology 2024Quote: ... rehydrated in graded alcohols and then blocked with 10% Horse serum in 5% BSA/PBS for 30 minutes at RT and incubated with a mouse anti-human DNase I antibody (1/100, Santa Cruz, overnight at 4 degrees). Sections were washed ...
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bioRxiv - Neuroscience 2024Quote: ... The blots were incubated in 2% BSA/Tris-buffered saline tween-20 (TBST) with primary antibodies overnight at 4°C: anti-HSP90 (Santa Cruz, sc-7947, 1:5,000), anti-SEL1L (home-made ...
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bioRxiv - Molecular Biology 2024Quote: ... the blotted membranes were incubated overnight at 4 °C with primary antibodies of anti-c-Myc (1:500) (sc-40, Santa Cruz Biotechnology, Dallas, TX, USA), GAPDH was used as a loading control (G-9 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated on a shaker with the primary antibodies: rabbit polyclonal anti-Trk(C14) (1:1 000, Santa Cruz Biotechnology, #sc-11, RRID: AB_632554), mouse monoclonal anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH ...
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bioRxiv - Biochemistry 2024Quote: ... The immunodetection of BACE1 was carried out by incubating cells with mouse anti-BACE1 primary antibody diluted 1:200 in PBS (Santa Cruz Biotechnology, Inc., Dallas, USA). After incubation with primary antibody overnight ...
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bioRxiv - Cell Biology 2024Quote: Samples were permeabilized with 0.5% Triton X100 for 90-120 s and stained with anti-α-tubulin antibodies conjugated directly with AlexaFluor647 (Santa Cruz, Cat. Nr. sc-23948), at a concentration of 2 µg/mL in 1% PBST (PBS containing 0.05% Tween-20) ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies in 2% BSA in PBS overnight at 4°C: mouse anti-PPARγ (Santa Cruz Biotech, sc-7273, 1:1,000), rabbit anti-CEBPα (Santa Cruz Biotech ...
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bioRxiv - Systems Biology 2022Quote: ... the membrane was washed 3 times with PBS-T buffer and then incubated at room temperature for 1 h with secondary antibodies: mouse monoclonal anti-rabbit IgG-HRP (Santa Cruz Biotechnology, cat. no. sc-2357), goat monoclonal anti-mouse IgG HRP (Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2020Quote: ... The membrane was blocked with 5% skim milk (Morinaga, Japan) for 60 min and incubated with the antibody against Epo (Santa Cruz, sc-5290, 1:500-2,000) for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
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bioRxiv - Cancer Biology 2020Quote: Sections from formalin fixed paraffin embedded mouse tissue or a human pancreatic cancer tissue microarray (Biomax PA961e) were stained with antibodies against PC (Santa Cruz sc-67021, 1:50 dilution) or ME1 (Proteintech 16619-1-AP ...
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bioRxiv - Neuroscience 2021Quote: The number of young differentiating neurons were identified with an antibody against DCX (1:1000; polyclonal goat-anti-DCX; Santa Cruz Biotechnology, Santa Cruz, CA, USA). The sections were incubated with the primary antibody in 0.01M PBS for 3 days at 4°C ...
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bioRxiv - Bioengineering 2020Quote: The following commercial primary antibodies were used at a concentration of 1:500: (i) βIII-tubulin (AA10, sc-80016; Santa Cruz Biotechnology, Inc., Dallas TX, USA), (ii ...
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bioRxiv - Microbiology 2022Quote: ... Briefly 3 x105 cells were incubated on ice with mouse anti-human ACE2 monoclonal antibody conjugated to AF-647 (Santa Cruz Biotechnology, USA, cat. SC-390851) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently incubated with primary antibodies against Pax7 (Developmental Studies Hybridoma Bank, 2 μg/mL) and MyoD (Santa Cruz Biotechnology, clone G-1, 1:200) diluted in 2.5% BSA and 2.5% GS in PBST ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated at 4°C overnight with 2 μg/ml each of anti-ERα(H222) rat IgG1 monoclonal antibody (mAb) (Santa Cruz Biotech, sc-5349, 1:100) and HA-probe (F-7 ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and incubated at room temperature for 1 hour in the dark with a fluorescent secondary antibody mixture contaning mouse IgGk BP-CFL594 (Santa Cruz Biotech, sc-516178, 1:100) and anti-rat IgG AF488 (ThermoFisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... Other sections were subjected to indirect immunoperoxidase staining with the following primary antibodies: anti-tartrate-resistant acid phosphatase (anti-TRAP).(SC-30833, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-receptor activator of nuclear factor kappa-B ligand (anti-RANKL ...
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bioRxiv - Developmental Biology 2021Quote: Amputated fins were fixed with 4% paraformaldehyde overnight at 4 °C and used for whole-mount immunofluorescence with a rabbit anti-phospho-histone-H3 primary antibody (#SC-8656R; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) to detect proliferative cells or with a chicken anti-GFP antibody (ab13970 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Developmental Biology 2023Quote: ... pH 8.0) for 1 hour at room temperature and incubated overnight in primary antibody (1:100 anti-Dnmt1 polyclonal, Santa Cruz, 1:1000 H3 polyclonal, Santa Cruz) diluted in blocking buffer at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted using SATB1 antibodies (custom-made anti-long in a 1:200 dilution and Santa Cruz sc-376097 in a 1:200 dilution) or anti-GFP (Minotech Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... All perfusion and staining procedures described above were used until incubation with primary rabbit anti-c-Fos antibody (1:500; Santa Cruz, sc-52, Dallas, TX, USA), after which the sections were incubated for 1 h with secondary Alexa Fluor 488 donkey anti-rabbit antibodies (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... A subset of sections from brains with CaMKII-SomArchon expression were stained with a mouse anti-CaMKIIα/β/γ/δ antibody (sc-5306, Santa Cruz, 1:50) followed by Alexa Fluor 568 goat anti-mouse secondary antibody (No ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were stained with the following primary antibodies diluted in Intercept (TBS) Blocking Buffer (LiCor, 927-60001): mouse anti-BRAF (Santa Cruz Biotech, sc-5284, 1:200), mouse anti-ERK (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... was blocked in 5% milk for one hour at room temperature and incubated with a monoclonal mouse anti-GFP antibody (Santa Cruz Biotechnology sc-9406, 1:2500), a rabbit anti-pRab8A phospho T72 monoclonal antibody (Abcam 230260 ...
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bioRxiv - Cancer Biology 2022Quote: ... with the respective primary antibody (KRAS-LS-Bio, LS-C175665; 1:4000; α-SMA-Abcam, ab7817, 1:250; GAPDH-Santa Cruz Biotechnology, sc-47724, 1:10000), diluted in 5% non-fat milk in PBS+ 0.5% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... The rest of the supernatants was subjected to overnight immunoprecipitation with 10 μg of IgG (Milipore, 12-371B; 1 μg/μl) or SF3B1 antibody (Santa Cruz, sc-514655; 0.2 μg/μl). Next day ...
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bioRxiv - Physiology 2024Quote: ... Primary antibodies against MyBP-HL (Barefield, 2022; 1:2500, (Barefield et al., 2022)) and cMyBP-C (Santa Cruz Tech, E7; Cat. No: sc-137180; 1:2500) were incubated in blocking buffer overnight at 4 °C ...
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bioRxiv - Genetics 2024Quote: ... The dried sections were incubated with corresponding antibodies (αGal, Cat. no. ALX-650-001F-MC05, Enzo; hCD55, Cat. no. ab230638, Abcam; hTBM, Cat. no. sc-13164, Santa Cruz; hCD39, Cat. no. ab223842, Abcam). For visualization of hCD55 ...
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ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genesbioRxiv - Genomics 2024Quote: ... was aliquoted into each of 3 tubes with antibody (2µg anti-HA Invitrogen #26183, 8µg anti-SP1 Santa Cruz Biotechnology sc-17824 X, or mock IP) and incubated with end-over-end rotation at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was detected using primary anti-CFTR antibodies 570 or 596 (Riordan lab UNC, Cystic Fibrosis Foundation, diluted 1:1000) and anti-β-actin (C4, Santa Cruz Biotechnology, diluted 1:2000). Secondary anti-mouse poly-HRP was added for chemiluminescent detection with HRP substrate (CFTR:Femto ...
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bioRxiv - Molecular Biology 2024Quote: ... the blots were washed 3 times in 1X TBST and then incubated in secondary mouse and rabbit antibodies (m-IgGκ BP-HRP, Santa Cruz sc-516102, 1:10,000; mouse anti-Rabbit IgG-HRP, Santa Cruz sc-2357, 1:10,000, respectively). The western blots were developed using Clarity Western ECL substrate (Bio-Rad 170–5061 ...
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bioRxiv - Microbiology 2024Quote: ... containing 10% milk for one hour and then incubated overnight at 4°C with anti-PARP-1 mouse monoclonal antibody C2-10 (Santa Cruz Biotechnologies sc-53643, 1/500) or a rabbit polyclonal IgG (Santa Cruz Biotechnologies sc-7150 1/1000) ...
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bioRxiv - Molecular Biology 2024Quote: ... and fixed in 4% PFA in PBS (15-20 min at RT) and processed for ICC using a primary anti-CDKL5 antibody (Santa Cruz Biotechnology, sc-376314; see above). The treatment with 1,6-Hex was omitted in control cultures ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBST-milk before incubation with species-specific HRP-conjugated secondary antibodies (m-IgGκ BP-HRP Santa Cruz, 616102 1:10000 dilution; or Mouse anti-Rb IgG HRP, Santa Cruz 2327 1:10000 dilution). SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... They were further incubated overnight in the same blocking buffer with one of the following primary antibodies: mouse monoclonal anti-LRRC8A (Santa Cruz, cat. #sc-517113, dilution 1:500), rabbit polyclonal anti-GAD65/67 (Millipore-Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 0.3 % TritonTM X-100 in PBS at room temperature) and then incubated overnight with the primary antibody (4° C, rabbit anti-cFos, Santa Cruz Biotechnology, Inc., sc-52, 1:500) in carrier solution (1 % Bovine serum albumin ...
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bioRxiv - Molecular Biology 2020Quote: ... the PVDF membrane was blocked with 5% (w/v) skimmed milk followed by an incubation with a primary antibody synthesized in mouse against GFP (Santa Cruz Biotechnology, CAT. #sc-9996; 1:600) or HA (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... for 1 hour at room temperature (RT) before incubating overnight at 4°C with the primary antibody for c-Myc (Santa Cruz Biotechnology sc-40, Dallas, Texas USA).Secondary goat anti-rabbit antibody (1:10000 dilution ...
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bioRxiv - Pathology 2023Quote: ... all membranes were stripped and re-probed for β-actin using anti-β-actin antibody at a concentration of 0.5 μg/ml (sc-47778, Santa Cruz Biotechnology Inc., Dallas, TX, USA). We used the inbuilt software of the C-Digit Blot Scanner for quantification.
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bioRxiv - Plant Biology 2024Quote: ... membranes were incubated with appropriate antibodies diluted in blocking buffer (α-FLAG: Cohesion Biosciences, at 1:3000 dilution; α-Myc: Santa Cruz Biotechnology, at 1:5000 dilution) overnight ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by immunoblotting using an anti-KRAS antibody (1:1000, WH0003845MI for G12D, Sigma-Aldrich; and 1:2000, F234 for G12V, Santa Cruz Biotechnology, Inc., Dallas, TX, USA). The immunoreactive bands were detected ...
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bioRxiv - Microbiology 2024Quote: ... containing 4% nonfat milk for 1 hr at room temperature and probed with primary antibodies: 1) anti-HA-probe (Santa Cruz Biotechnology, Cat# sc-7392, 1:500), 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... and western blot (WB) analysis: ANXA4 (HPA007393, Atlas Antibodies, Sweden – WB: 1:1000, IF: 1:300, IHC: 1:300 and sc-46693, Santa Cruz Biotechnology, USA – WB: 1:1000), ANXA2 (#8235 ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes were blocked with 5% milk in TBS containing 0.1% tween (TBS-t) and incubated overnight at 4°C with the primary antibodies: Vinculin (Santa Cruz Biotechnology, Cat No. sc-73614, 1:500) and FLAG M2 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were lysed in RIPA buffer and immunoblotting was performed using primary antibodies: XPO1 (CRM1 rabbit polyclonal H-300; sc-5595, Santa Cruz or CRM1 mouse monoclonal (C-1): sc-74454) ...