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Citations for Santa Cruz :
5051 - 5100 of 5356 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: goat anti-LCN2 (R&D AF1747), E-cadherin (Cell Signal, 3195T) IL-6 (Santa Cruz Biotechnology, sc-1265).
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bioRxiv - Developmental Biology 2020Quote: ... washed with TBS-T and incubated overnight with primary antibodies (anti-Actin 1:10000 Santa Cruz Biotechnology #sc1616; anti-Dab1 1:2000 Sigma-Aldrich #HPA052033, anti-Fyn 1:1000 Santa Cruz Biotechnology #sc-16 ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 5% skimmed milk powder for 2 hours at room temperature.Incubate primary antibody overnight at 4°C.The membrane was washed three times with TBST and then incubated at room temperature with conjugated HRP secondary antibodies for 2 hours.ECL (enhanced chemiluminescence kit, Santa Cruz Biotechnology, Dallas, TX) detection system (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was stopped by the addition of 8X SDS loading buffer and unmodified and ubiquitinated recombinant TOP2B was detected using His antibody (sc-8036, Santa Cruz biotechnology, USA). For E2 ubiquitin-conjugating enzyme screening ...
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bioRxiv - Cancer Biology 2021Quote: ... PVDF membranes were blotted using semi-dry Trans-Blot® Turbo™ Transfer System and analyzed using anti-NAMPT (1:200) monoclonal antibody (Santa Cruz).
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bioRxiv - Microbiology 2020Quote: ... Slides were blocked in normal serum and incubated in primary antibody (USP13 (D4P3M; 12577, Cell signalling Technologies (CST)) or Mcl-1 (S-19; sc-819, Santa Cruz Biotechnology (SCBT)) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... were washed in 500 μl tris buffered saline+0.05% NP40 wash buffer containing 8 μl ZNF804A antibody or 8 μl control Rabbit immunoglobulin (IgG) (Santa Cruz Biotechnology, sc-2027). Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific ...
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bioRxiv - Pathology 2021Quote: ... we performed immunohistochemistry of MUC5B for human tracheal serial sections with the antiserum (M-25, 1:1000) and previously established commercial rabbit anti-MUC5B antibody (H-300, Santa Cruz, 1:1000). Staining with these antibody and antiserum appeared identical ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with blocking buffer (5% normal goat serum and 0.3% Triton X-100 in PBS) for one h at room temperature and incubated with primary antibody (c-fos, 1: 1000, Santa Cruz, CA, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... samples had unspecific sites blocked for 1h in a 5% fetal bovine serum albumin solution of washing buffer to be next incubated overnight at 23°C with a polyclonal rabbit anti c-Fos antibody (1:1000 washing buffer, Santa Cruz Biotechnology, USA). After successive washing ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight rotating at 4 °C together with either α-KAP1 or α-RIF1 antibodies (see Supplementary Table 2) or IgG only control (#sc-2026, Santa Cruz), 10% of chromatin was isolated as input control ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used in this study: phospho-STAT1 (pY701.4A) mouse monoclonal IgG2aĸ targeting to phosphorylated Tyr701 (Santa Cruz Biotechnology #sc-136229), STAT1 p84/p91 (E-23 ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 3 times with PBS and incubated with 1:400 secondary antibody donkey IgG anti–goat CFL 488 (SC362255, Santa Cruz Biotechnology, USA) for 1 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: Western blotting of nuclear extracts or whole cell extracts was performed by standard methods using antibodies specific for the N-terminal portion of ETV6 (Sigma, # HPA000264; Santa Cruz, #sc-166835x), CTCF (Cell Signaling ...
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bioRxiv - Genomics 2022Quote: ... Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz, SC-373750, 1:250) or rabbit polyclonal anti-DNMT1 (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... The antibodies that were used were anti-acetylated alpha tubulin AlexaFluor 647 (cilia marker, clone 6-11B-1, sc23950, Santa Cruz, 1:2000) and ZO-1 monocolonal antibody AlexaFluor 488 (tight junction marker ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatants were analyzed by western blotting using a rabbit polyclonal antibody specific for MDM2 (1:1000, sc-965, Santa Cruz Biotechnology, Inc.) and GAPDH (1:20000 ...
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bioRxiv - Molecular Biology 2022Quote: ... it was incubated for 1 h at RT with species-specific horseradish peroxidase (HRP)-coupled secondary antibody (Santa Cruz, sc-2005, goat anti-mouse; Santa Cruz, sc-2357, mouse anti-rabbit; Santa Cruz, sc-2020 ...
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bioRxiv - Microbiology 2023Quote: ... in TBS-T (Tris-buffered saline, 0.1% Tween 20) and incubated with anti-TRIM28 antibody (1:3,000) (sc-515790, Santa Cruz Biotechnology, Dallas, TX, USA), anti-TRIM33 antibody (1:1,000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... solvent control and drug treatment groups with Trypsin-EDTA (2X) and single cell suspensions were stained using an antibody against TMPRSS2 (Santa Cruz, sc-515727). Samples were fixed with 4 % paraformaldehyde which was followed by permeabilization step or 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were blocked for 3h at RT and incubated with primary antibodies (rabbit anti-mouse NF-κB(RelA/p65/) (sc-8008; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-mouse phospho(p)-RelA/p65(NF-κB ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of anti-Histone H3K9me3 (2F3) and anti-H3K9ac (1qE5) antibodies (provided by H. Kimura) [84] and an equal amount of normal mouse IgG (Santa Cruz, sc-2025) were added to the nuclear extract and incubated overnight at 4ºC with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated with primary antibodies (anti-FBL, 1:500, Novus Biologicals, NB300-269; anti-RPA194, 1:50, Santa Cruz, sc-48385; anti-UBF, 1:50, Santa Cruz, sc-13125 ...
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bioRxiv - Neuroscience 2023Quote: ... Then purified primary antibody against ERα/ERβ (1:400, SC-8005, Santa Cruz Biotechnology, USA;1:400, SC-53494, Santa Cruz Biotechnology, USA) in 1% BSA was incubated overnight at 4□ ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight in primary antibody solution with goat polyclonal anti-NMDAa2 (GluN2B) (1:250; sc-1469, Santa Cruz Biotechnology, Santa Cruz, USA) in 0.2% PBS-Tx with 1% normal serum and 20% biotin ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 in 1XTBS) for 1 h at RT followed by incubation with primary antibodies against ERG (Santa Cruz, 1:100) for 72 h and EC marker ...
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bioRxiv - Physiology 2024Quote: ... A commercially available monoclonal antibody was used to detect mitochondrial creatine kinase (mtCK C-1 43 kDa; Santa Cruz 376320, 1:500 dilution).
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bioRxiv - Cancer Biology 2024Quote: ... The blots were blocked for 30 min with TBST-5% milk and then probed overnight with primary antibodies against hnRNP U (1:1000, Santa Cruz sc-32315), β-Actin (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated at RT with primary antibodies (RNA Polymerase II, rabbit polyclonal, 1:1000, Abcam, ab5131; Treacle, mouse monoclonal, 1:500, Santa Cruz, sc-374536) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... Duplicate samples (0.5 μL) were subjected to SDS-PAGE and analyzed by western blot with an anti-Ubiquitin antibody (Santa Cruz Biotechnology, sc-8017). When using full length MIB1 protein ...
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bioRxiv - Developmental Biology 2023Quote: ... with a cocktail of protease inhibitors and the soluble whole cell lysates were immunoprecipitated with an anti-Myc antibody (Santa Cruz, sc-40). The immunoprecipitated proteins were then analysed by Western blot.
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bioRxiv - Immunology 2022Quote: ... the blotted membranes were then incubated overnight with primary antibodies (anti-Blimp1 (C14A4, 1:1,000, Cell Signaling Technologies or C-7, 1:200, Santa Cruz Biotechnology), anti-β-actin (A5441 ...
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bioRxiv - Immunology 2023Quote: ... sEH (1:200, custom made antibody generated by Proteintech Group, Shanghai, China as previously described[37]; or 1:100, sc-166961, Santa Cruz, Texas, US), cTNT (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then blocked with 3% BSA and 0.02% Tween 20 for 1 h at room temperature and incubated overnight at 4°C with anti-G3BP1 antibodies (1:250; SC-81940, Santa Cruz Biotechnology, CA), followed by a 1-h incubation with an Alexa488-conjugated anti-immunoglobulin secondary antibody (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... Tween-20] at room temperature for 90 min and then incubated at 4°C overnight with the primary antibodies in blocking buffer (Alexa Fluor 594 anti-OCT3/4, 1:200 v/v; Santa Cruz, sc-5279). The cells were then washed and incubated at 25°C for 60 min with the secondary antibody (Alexa Fluor 594 Goat anti-mouse IgG ...
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Dynamic Effects of Ventral Hippocampal NRG3/ERBB4 Signaling on Nicotine Withdrawal-Induced ResponsesbioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature before reacting overnight at 4°C with primary antibodies: Neuregulin-3 (NRG3) (1:500, sc-67002, N-terminal extracellular domain, Santa Cruz Biotechnology, Santa Cruz, CA), ERBB4 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... GTP-bound GFP-ROP2 or -ROP6 that was associated with the MBP-RIC1 beads was used for analysis by western blotting with anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA). Meanwhile ...
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bioRxiv - Biochemistry 2023Quote: ... incubated the supernatant with corresponding antibody for 12h at 4°C and then incubated with A/G plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The membranes were blocked with 5% BSA or 5% dry milk in TBS-T for 1h at room temperature and incubated with a primary antibody for an overnight at the following dilution: Adar1 1:200 (to detect mouse Adar, Santa Cruz, #sc-73408), Pkr 1:2,000 (Abcam #ab184257 [EPR19374]) ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... using a homogeneous protocol of [35S]GTPγS scintillation proximity assay coupled to the use of the following antibodies: mouse monoclonal anti-Gαi1 (1:20, Santa Cruz Biotechnology #sc-13534), rabbit polyclonal anti-Gαi2 (1:20 ...
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bioRxiv - Bioengineering 2023Quote: ... slides were blocked with serum and biotin/avidin followed by incubation of primary antibodies: Mouse anti-Fah (40) (1:600) or mouse anti-Hpd (Santa Cruz, 1:100). Slides were developed using the 3,3’- diaminobenzidine chromogen kit (DAB ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Slides were washed in dPBS three times and stained with primary antibodies: goat anti-human neutrophil elastase (Santa Cruz Biotech 9520, 1:1000), and goat anti-human myeloperoxidase (Abcam 9535 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3 washes with BB and incubation with anti-Tom20 antibodies conjugated with Alexa 647 fluorophore (Santa Cruz Biotechnology Inc.; 1:100) for 90 min at RT ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... for 30 min at 60°C followed by Duolink® In Situ PLA® blocking solution for 1 h at 37°C and incubated with primary antibodies against LRP1(Cat#: sc-57353, Santa Cruz Biotechnology), leptin (Cat# ...
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bioRxiv - Physiology 2023Quote: ... Histological sections were incubated overnight at 4°C with the primary antibodies (rabbit anti-human COX1, ab133319, abcam, 1:200; rat anti-mouse Endomucin, sc-5495 Santa Cruz, 1:200), then incubated with Texas Red-conjugated goat anti-Rat (TI-9400 Vector Labs ...
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bioRxiv - Microbiology 2023Quote: ... Input and output fractions were resolved on SDS-PAGE and subjected to immunoblot analysis using anti-Myc antibodies (Santa Cruz Biotechnology; sc-40) or anti-GST (Santa Cruz Biotechnology ...
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bioRxiv - Pathology 2023Quote: ... β-actin was used as an internal control and its expression was determined using a specific antibody (1:1,000, SC-47778, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with 1% PBS/BSA and incubated in 1% PBS/BSA with anti-TRA-1-60-antibody mouse monoclonal IgM (1:100, Santa Cruz Biotechnology, Inc.) for 20 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... cultures were incubated overnight at 4ºC with the indicated diluted primary antibodies: anti-α-SMA (1:200, Triton X-100; Santa Cruz, sc-53015); anti-FAP (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... Fibroblasts were incubated with primary antibody solution in 5% BSA overnight at 4°C in a moisture chamber: anti-ALIX (1:200; Santa Cruz, sc-53540), anti-Amyloid-β (1:100 ...