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5001 - 5050 of 8287 citations for 6 Phenyl 1H imidazo 1 2 b pyrazole since 2019

• Desert Hedgehog-driven endothelium integrity is enhanced by Gas1 but negatively regulated by Cdon

  Candice Chapouly, et al., bioRxiv - Cell Biology 2020

  Quote: Expression of human ICAM-1 and VCAM-1 expression were evaluated by SDS PAGE using mouse anti-human I-CAM1 antibodies (Santa Cruz Biotechnology, Cat#sc-8439) and rabbit anti-VCAM-1 (Abcam ...
• Myogenin controls via AKAP6 non-centrosomal microtubule organizing center formation at the nuclear envelope

  Robert Becker, et al., bioRxiv - Cell Biology 2021

  Quote: ... Samples were diluted 1:5 in RIPA buffer and incubated overnight at 4°C with 1 µg/ml myogenin antibody (Santa Cruz Biotechnology, #sc-12732 X) or IgG1 isotype control (Thermo Fisher ...
• Anterior thalamic nuclei neurons sustain memory

  S. C. Barnett, et al., bioRxiv - Neuroscience 2021

  Quote: ... before 72 h incubation at 4°C with rabbit polyclonal zif268 antibody (also known as Egr-1; 1:1500; Santa Cruz Bio, cat# SC-189) in PBS-Tx with 1% normal goat serum (NGS) ...
• Prediction of sex-determination mechanisms in avian primordial germ cells using RNA-seq analysis

  Kennosuke Ichikawa, et al., bioRxiv - Developmental Biology 2022

  Quote: ... The gonad cells and blood cells were incubated with anti-stage-specific embryonic antigen-1 (SSEA-1) antibody (sc-2170; Santa Cruz Biotechnology, Dallas, TX, USA) diluted with wash buffer (0.5% BSA and 0.1% NaN3-PBS ...
• Amplification of potential thermogenetic mechanisms in cetacean brains

  Paul R. Manger, et al., bioRxiv - Neuroscience 2020

  Quote: ... The blots were incubated over night at 4°C under gentle agitation in the primary antibody solutions (1:300 goat anti-UCP1, Santa Cruz Biotechnology, sc-6528 or 1:300 goat anti-UCP4, Santa Cruz Biotechnology, sc-17582). The blots were washed for 3 x 10 min in 1 x Animal-Free Blocker and incubated for 1 h at room temperature in HRP-conjugated rabbit anti-goat secondary antibody (1:1000 ...
• Progression and Resolution of SARS-CoV-2 Infection in Golden Syrian Hamsters

  Kathleen R. Mulka, et al., bioRxiv - Pathology 2021

  Quote: ... VA), anti-CD3 antibody (1:200; DAKO; Ref: A0452 Santa Clara, CA, or anti-pan-cytokeratin antibody (1:1000; Santa Cruz; sc-8018 Dallas, TX). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems ...
• The type 2 diabetes gene product STARD10 is a phosphoinositide binding protein that controls insulin secretory granule biogenesis

  Gaelle R. Carrat, et al., bioRxiv - Cell Biology 2020

  Quote: ... the membrane was incubated 1 h with the horseradish peroxidase conjugated donkey anti-goat IgG (Santa Cruz sc2020; 1/2000 in TBS-T + 3%BSA). After 5 washes of 10 min ...
• The type 2 diabetes gene product STARD10 is a phosphoinositide binding protein that controls insulin secretory granule biogenesis

  Gaelle R. Carrat, et al., bioRxiv - Cell Biology 2020

  Quote: ... in TBS-T and probed for 1 h with the polyclonal goat anti-STARD10 antibody (Santa Cruz sc54336; 1/1000 in TBS-T + 3%BSA). After 5 washes of 10 min ...
• Epoxy fatty acid dysregulation and neuroinflammation in Alzheimer’s disease is resolved by a soluble epoxide hydrolase inhibitor

  Anamitra Ghosh, et al., bioRxiv - Neuroscience 2020

  Quote: ... or TBS with 5% bovine serum albumin (BSA) followed by antibody incubation: sEH (1:300, Santa Cruz or 1:1000, Dr. Bruce Hammock’s lab (26)) ...
• Gene editing is suitable to treat GM1 Gangliosidosis: a proof-of-concept study

  Delphine Leclerc, et al., bioRxiv - Genetics 2022

  Quote: ... and then 1 hour at room temperature with the HRP linked secondary antibody (HRP-linked anti-rabbit IgG, #7074 Santa Cruz, dilution 1/2000; HRP-linked anti-mouse IgG, #7076 Santa Cruz, dilution 1/2000). Signal was detected with Amersham ECL select substrate (Cytiva ...
• Lithium treatment reverses irradiation-induced changes in rodent neural progenitors

  Zanni Giulia, et al., bioRxiv - Neuroscience 2019

  Quote: ... The following primary antibodies were used: rat anti-BrdU (1:500, AbD Serotec, Kidlington, UK) and goat anti-DCX (1:100, Santa Cruz Biotechnology Inc., CA, USA). The following secondary antibodies were used ...
• Single Cell Profiling of Acute Kidney Injury Reveals Novel Transcriptional Signatures, Mixed Identities and Epithelial-to-Stromal Crosstalk

  Valeria Rudman-Melnick, et al., bioRxiv - Developmental Biology 2019

  Quote: ... the protocol was modified by adding the primary antibody recognizing the target (Krt8, rabbit, Abcam, EP1628Y, 1/100; Aqp1, mouse, Santa Cruz, sc-25287, 1/50) to the anti-DIG-AP antibody mixture and incubation O/N at 4 °C (Lopez ...
• A highly expressing, soluble, and stable plant-made IgG fusion vaccine strategy enhances antigen immunogenicity in mice without adjuvant

  Andrew G. Diamos, et al., bioRxiv - Immunology 2020

  Quote: ... IgG2a antibodies were measured from sera diluted 1:100 in 1% PBSTM and detected with IgG2a horseradish peroxidase conjugate (Santa Cruz Biotechnology, Dallas, TX, USA). IgG2a values were normalized by total IgG ...
• β-catenin Signaling Regulates Cell Fate Decisions at the Transition Zone of the Chondro-Osseous Junction During Fracture Healing

  Sarah Anne Wong, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Biotinylated species-specific secondary antibodies were diluted in PBS with 5% GS and applied to tissues for 1 hr at room temperature (Santa Cruz, #sc-2041, 1:250). Staining was detected using the VectaStain ABC Elite Kit (#PK-6100 ...
• Small-molecule Polθ inhibitors provide safe and effective tumor radiosensitization in preclinical models

  Gonzalo Rodriguez-Berriguete, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Cells were then incubated overnight at 4°C using any of the following primary antibodies diluted in PBS 1% BSA: rabbit anti RAD51 (Santa Cruz # sc-8349; 1/1500), rabbit anti-γH2AX (Novus Biologicals #NB100-2280 ...
• SORORIN is an evolutionary conserved antagonist of WAPL

  Ignacio Prusén Mota, et al., bioRxiv - Cell Biology 2022

  Quote: ... PK-tagged proteins were detected using mouse-anti-PK (V5) antibody (Serotec; 1:2000 dilution) and goat anti-mouse IgG-HRP secondary antibody (Santa Cruz Biotechnology; 1:5000 dilution) in 0.1% PBS-T ...
• Reduced oocyte quality and exacerbation of the maternal age effect are enduring consequences of low-level atrazine exposure in mouse

  Yan Yun, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: Immunofluorescence staining was performed using the following primary antibodies with incubation overnight at room temperature after blocking at room temperature for 1 hour: rabbit anti-SYCP3 (Santa Cruz, sc-33195; 1:200), mouse anti-MLH1 (Cell Signaling Technology ...
• Genome-wide DNA methylation changes in human spermatogenesis

  Lara M. Siebert-Kuss, et al., bioRxiv - Genetics 2023

  Quote: ... The remaining cells were incubated with fluorophores-conjugated primary antibodies at room temperature for 1 h against DMRT1-AI647 (1:200, Santa Cruz, Cat# sc-377167 AF647), MAGEA4-Dy550 (1:20 ...
• Coordinated regulation of photosynthesis and translation via NIK1/RPL10/LIMYB signaling module in response to biotic and abiotic stresses

  Ruan M. Teixeira, et al., bioRxiv - Plant Biology 2023

  Quote: ... and an α-GFP antibody (α-GFP, life technologies, cat # A11122, 1:5000; goat anti-mouse IgG-HRP, Santa Cruz, Cat # sc-2005, 1:10,000). Likewise ...
• Molecular fingerprints in the hippocampus of alcohol seeking during withdrawal

  Roberto Pagano, et al., bioRxiv - Animal Behavior and Cognition 2023

  Quote: ... After washing in TBST the membranes were incubated in a secondary antibody with HRP (anti-rabbit, Vector pI-1000, 1:5000; anti-mouse, Santa Cruz, sc-2005, 1:5000) and washed again ...
• Learning transcriptional and regulatory dynamics driving cancer cell plasticity using neural ODE-based optimal transport

  Alexander Tong, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 10% Horse Serum and 0.1% Triton O/N at 4 degrees with rotation. Following incubation with primary antibodies ESRRA (Cell Signalling Technology, E1G1J, 1:200, Cat no. 13826);ZEB1 (Santa Cruz, #H-102, 1:200);CDH1 (BD Biosciences/(36/E-Cadherin ...
• Cancer cells co-evolve with retrotransposons to mitigate viral mimicry

  Siyu Sun, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The LINE-1 ORF1 shRNA containing lentivirus medium was added to cells with 8 µg ml-1 of polybrene (Santa Cruz Biotechnology, cat# sc-134220). Approximately 48 hrs after infection ...
• Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover

  Michael J. Podolsky, et al., bioRxiv - Cell Biology 2023

  Quote: ... Membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies (1:4000: Cell Signaling Technology; 1:2,000: R&D Systems, Bio-Techne; Santa Cruz Biotechnology) for 1 hour at room temperature ...
• The Hippo/YAP Pathway Mediates the De-differentiation of Corneal Epithelial Cells into Functional Limbal Epithelial Stem Cells In Vivo

  Yijian Li, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit anti-p-Histone H3-Ser10 (pH3) monoclonal antibody (CST, #53348; 1:100) and mouse anti-CK15 monoclonal antibody (Santa Cruz, sc-47697; 1:200). The following secondary antibodies were used ...
• The SARS-CoV-2 spike protein binds and modulates estrogen receptors

  Oscar Solis, et al., bioRxiv - Immunology 2022

  Quote: ... for 5 minutes and incubated at room temperature for 1 hour in the dark with a fluorescent secondary antibody mixture contaning mouse IgGk BP-CFL594 (Santa Cruz Biotech, sc-516178, 1:100) and anti-rat IgG AF488 (ThermoFisher Scientific ...
• S. cerevisiae Srs2 helicase ensures normal recombination intermediate metabolism during meiosis and prevents accumulation of Rad51 aggregates

  Laura J Hunt, et al., bioRxiv - Genetics 2019

  Quote: ... skimmed milk in PBS at the following concentrations for 30-120 min at RT (anti-goat, Santa Cruz sc-2020, 1:2500; anti-mouse, Santa Cruz sc-2005, 1:2500). Membranes were washed 3x in PBS ...
• Toxoplasma type II effector GRA15 has limited influence in vivo

  Emily F. Merritt, et al., bioRxiv - Microbiology 2024

  Quote: ... Cells were then blocked in 3% goat serum for 1 hour at room temperature and incubated in mouse anti-Sag1 (28) [DG52] (gift from John Boothroyd, 1:5000) and anti-NF-κB (p65) (Santa Cruz Biotechnology, sc-372, 1:1000) antibodies overnight at 4°C ...
• A novel SATB1 protein isoform with different biophysical properties

  Tomas Zelenka, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The membrane was blotted using SATB1 antibodies (custom-made anti-long in a 1:200 dilution and Santa Cruz sc-376097 in a 1:200 dilution) or anti-GFP (Minotech Biotechnology ...
• MYBPHL nonsense mutations have poor sarcomere binding, are degraded, and cause abnormal contraction

  Alejandro Alvarez-Arce, et al., bioRxiv - Physiology 2024

  Quote: ... Primary antibodies against MyBP-HL (Barefield, 2022; 1:2500, (Barefield et al., 2022)) and cMyBP-C (Santa Cruz Tech, E7; Cat. No: sc-137180; 1:2500) were incubated in blocking buffer overnight at 4 °C ...
• Poly (ADP-ribose) polymerase-1 regulates HIV-1 replication in human CD4+ T cells

  Zachary S. Martinez, et al., bioRxiv - Microbiology 2024

  Quote: ... containing 10% milk for one hour and then incubated overnight at 4°C with anti-PARP-1 mouse monoclonal antibody C2-10 (Santa Cruz Biotechnologies sc-53643, 1/500) or a rabbit polyclonal IgG (Santa Cruz Biotechnologies sc-7150 1/1000) ...
• SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

  Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

  Quote: ... Mouse anti-P65/RELA (Santa Cruz: sc-8008, IF – 1:100); Rabbit anticGAS (Atlas Antibodies ...
• Constitutive activation of the PI3K-Akt-mTORC1 pathway sustains the m.3243A>G mtDNA mutation

  Chih-Yao Chung, et al., bioRxiv - Cell Biology 2021

  Quote: ... and anti-β-actin (1:10000, Santa Cruz Biotechnology #sc-47778).
• Combined oncolytic adenovirus carrying MnSOD and mK5 genes both regulated by survivin promoter has synergistic inhibitory effect on gastric cancer

  Shanshan Liu, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Cleaved-PARP (1:10000) (purchased from Santa Cruz Biotechnology. CA. USA), Caspase-8 (1:1000) ...
• EIPR1 controls dense-core vesicle cargo retention and EARP complex localization in insulin-secreting cells

  Irini Topalidou, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse monoclonal anti-c-Myc (1:1000, Santa Cruz, sc-40), mouse monoclonal anti-beta-tubulin (1:1000 ...
• Bbs5 functions as the docking compartment of Bbsome binding to Klc3 regulated by Bbs5-Ser²⁴⁶ PKC-phosphorylation during mouse spermatogenesis

  Dahai Yu, et al., bioRxiv - Cell Biology 2020

  Quote: ... and immunoblotted with antibodies to Bbs5 (1:200; Santa Cruz Biotechnology), phospho-Bbs5-pSer19 /nonphospho-Bbs5-Ser19 ...
• Local RNA translation controls cell migration and Rab GTPase function

  Konstadinos Moissoglu, et al., bioRxiv - Cell Biology 2020

  Quote: ... and anti-RABIF (1:1,500; Santa Cruz biotechnology, cat# sc-390759). After washing ...
• C-Jun N-terminal kinase (JNK) pathway activation is essential for dental papilla cells polarization

  Jiao Luo, Xiujun Tan, Ling Ye, Chenglin Wang, bioRxiv - Cell Biology 2020

  Quote: ... anti-rabbit IgG FITC conjugated (1:200, Santa Cruz, CA, USA). Cells at the leading edge of scratch line were observed ...
• Endogenous formaldehyde scavenges cellular glutathione resulting in cytotoxic redox disruption

  Carla Umansky, et al., bioRxiv - Cell Biology 2020

  Quote: ... 1 mM Glutathione monoethyl ester (GSH-MEE, Santa Cruz, # sc-203974); 0 and 1 mM Trolox (Sigma-Aldrich ...
• TRIP6 is required for tension at adherens junctions

  Srividya Venkatramanan, et al., bioRxiv - Cell Biology 2020

  Quote: ... and mouse anti-TRIP6 (1:100; Santa Cruz Biotechnology sc-365122). F-actin was stained with Alexa Fluor 647-conjugated phalloidin (1:50 ...
• Drosophila USP22/non-stop regulates the Hippo pathway to polarise the actin cytoskeleton during collective border cell migration

  Hammed Badmos, et al., bioRxiv - Cell Biology 2020

  Quote: ... Mouse anti-aPKC ζ (sc-17781, 1:200) from Santa Cruz. Guinea pig anti-Merlin (1:7500 ...
• Capturing limbal epithelial stem cell population dynamics, signature, and their niche

  Anna Altshuler, et al., bioRxiv - Cell Biology 2020

  Quote: ... Cd63 (Santa cruz sc-5275, IF-P/IF-Wmt 1:100), Ifitm3 (Abcam ab15592 ...
• Reconstitution of Alveolar Regeneration via novel DATPs by Inflammatory Niches

  Jinwook Choi, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-Hopx (1:100, Santa Cruz Biotechnology Inc., sc-30216), rabbit anti-Aqp5 (1:200 ...
• Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins

  Yulia Steblyanko, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-NuMA (F-11) (1:250; Santa Cruz, sc-365532), rabbit anti-HSET (1:500 ...
• Cytoprotection by a naturally occurring variant of ATP5G1 in Arctic ground squirrels

  Neel S. Singhal, et al., bioRxiv - Cell Biology 2020

  Quote: ... HSP90 (sc-69703, 1:1000, Santa Cruz Biotechnology, Dallas, TX, USA).
• Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast

  Galal Yahya Metwaly, et al., bioRxiv - Cell Biology 2019

  Quote: ... and mouse anti-HA (Y-11, Santa Cruz Biotechnology, 1:2,500) primary antibodies.
• Akt/Foxo pathway activation switches apoptosis to senescence in short telomere zebrafish

  Mounir El-Maï, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-p16 (F-12) (1:700, Santa Cruz Biotechnology, sc-1661); anti-SOD-2 (1:1000 ...
• SEMA3F-deficient colorectal cancer cells Promote lymphangiogenesis: fatty acid metabolism replace glycolysis for energy supply during lymphatic endothelial cells proliferation in tumor hypoxia microenvironment

  Xiaoyuan Fu, et al., bioRxiv - Cell Biology 2019

  Quote: ... including FITC-conjugated goat anti-rabbit IgG (1:50, Santa Cruz), FITC-conjugated goat anti-mouse IgG (1:50 ...
• Primary mammary organoid model of lactation and involution

  Jakub Sumbal, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse monoclonal anti-α-tubulin (Santa Cruz, sc-5286, 1:1000), and anti-mouse secondary antibody (Merck ...
• Identification and characterization of distinct murine brown adipocyte lineages

  Ruth Karlina, et al., bioRxiv - Cell Biology 2020

  Quote: ... β-actin (HRP-linked, Santa Cruz Biotechnology cat# sc-47778, 1:5000) was used as loading control ...
• BNIP3 phosphorylation by JNK1/2 promotes mitophagy via enhancing its stability under hypoxia

  Yun-Ling He, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-c-Myc (1:1,000, rabbit pAb, Santa Cruz, sc-789), anti-GFP (1:1,000 ...