Labshake search
Citations for Miltenyi Biotec :
151 - 200 of 3024 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... CD4 T cells were purified by negative selection using the human CD4 T Cell Isolation Kit (Miltenyi Biotec). T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2022Quote: ... the medium was removed and T-cells were isolated with the Pan T-cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol using magnetic activated cell sorting (MACS) ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from PBMC using CD4+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions (average purity of 95% ...
-
bioRxiv - Immunology 2020Quote: ... splenic T-cells from donor mice were enriched using the Pan T-cell Isolation Kit II (Miltenyi Biotec), while bone marrow (BM ...
-
bioRxiv - Cancer Biology 2020Quote: Mouse splenic T cells were isolated from Ptch1+/-:Tp53-/- mice using Pan T cell isolation Kit (Miltenyi Biotec). 4 × 104 mouse T cells were labeled with CFSE (Life Technologies ...
-
bioRxiv - Immunology 2022Quote: ... and CD4+ or CD8+ T cells were isolated using CD4+ or CD8+ T cells isolation kits (Miltenyi Biotec) as described in the manufacture protocols ...
-
bioRxiv - Immunology 2020Quote: ... Pan T cells were enriched through negative selection using magnetic beads (Pan T cell Isolation Kit, Miltenyi Biotec) as suggested by the supplier ...
-
bioRxiv - Immunology 2020Quote: ... Naïve CD8+ T-cells were isolated from autologous PBMC using “Naive CD8+ T Cell Isolation Kit” (Miltenyi Biotec) in parallel to the addition of MART-1 to the immature DC fraction ...
-
bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated using magnetic column separation (Miltenyi Biotec, CD4+ T cell isolation kit, human); 17.7 million CD4+ T cells ± 7.2 million were isolated from each subject ...
-
bioRxiv - Immunology 2022Quote: ... the mixed T cells were treated with the T cell activation/expansion kit (#130-093-627; Miltenyi Biotec). As controls ...
-
bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were isolated by MACS using the mouse CD4+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
-
bioRxiv - Cancer Biology 2022Quote: Cryopreserved bone marrow mononuclear cells were thawed and stained using standard procedures with the surface antibody CD34-PE (clone AC136, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
-
bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: Cryogenically preserved healthy human peripheral blood T-cells or murine splenic T-cells were magnetically isolated by negative selection using Pan T-cell isolation kits from Miltenyi Biotec. Murine splenocytes underwent RBC lysis (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
-
bioRxiv - Immunology 2023Quote: ... OT-I T cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) or using the EasySepTM Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies) ...
-
bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
-
bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated (Miltenyi Biotec), activated with anti-CD3/CD28 beads (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
-
bioRxiv - Immunology 2022Quote: ... Anti-biotin-PE antibody was purchased from Miltenyi Biotec (130-113-291) for biotin–LPETG SrtA substrate staining ...
-
bioRxiv - Cancer Biology 2021Quote: Determination of the ROR1 surface expression of T-REx™-293 and its derivates was performed using the anti-ROR1-APC (#130-119-860, Miltenyi Biotec) and Accuri C6 (BD Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were obtained by pre-enrichment using an “untouched” CD4+ T Cell Isolation Kit (Miltenyi Biotec) with subsequent flow cytometric analysis (CD4+ [clone RM4-5 ...
-
bioRxiv - Immunology 2022Quote: CD8 T cells were purified from PBMC by negative immunomagentic sorting using CD8+ T Cell Isolation Kit (Miltenyi Biotec) and used for bilayers experiments or for further separation into the subsets ...
-
bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2022Quote: ... Peripheral T- and B-cells were isolated from whole spleen using Pan T Cell (Miltenyi Biotec,130-095-130) or Pan B Cell (Miltenyi Biotec ...
-
bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
-
bioRxiv - Immunology 2022Quote: ... Mouse CD4+ T cell isolation kit and CD8+ T cell isolation kit were obtained from Miltenyi Biotec (Auburn, CA). Clodronate and control liposomes were obtained from Liposoma (Amsterdam ...
-
bioRxiv - Immunology 2022Quote: CD4 T cells were enriched from PBMCs by negative immunomagnetic selection using CD4+ T cell isolation kit (Miltenyi Biotec), according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2019Quote: ... T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in RPMI 1640 (Gibco ...
-
bioRxiv - Cancer Biology 2021Quote: ... The naïve T cells were purified from human PBMC using Pan T cell isolation kit (Miltenyi Biotec, Cat#130096535) and activated and expanded by CD3/CD28 Dynabeads (GibcoTM ...
-
bioRxiv - Immunology 2023Quote: ... T cells were purified from PBMC by magnetic-bead-based negative T-cell isolation (Miltenyi Biotec, Bergisch Gladbach, Germany) as described before (Wabnitz et al. ...
-
bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS/FCS and naïve CD4+ T cells were isolated using the naïve CD4+ T cell isolation kit (mouse) from Miltenyi Biotec according to their standard protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... The resulting cell suspension was washed twice with PBS and used for the isolation of naïve CD4+ T cells according to the manufacturer’s instructions (Naive T Cell Isolation Kit II; Miltenyi Biotec). Naive CD4+ T cell purity was assessed by staining for CD4 PerCPeFluor710 (clone ...
-
bioRxiv - Immunology 2024Quote: ZFP36L2CKO and WT OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: CD4+ T cells were separated from healthy controls PBMC using pan T cell isolation kit and magnetic-activated cell sorting (Miltenyi Biotec, USA). Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec ...
-
bioRxiv - Immunology 2020Quote: ... cells were stained with magnetic anti-biotin beads (Miltenyi Biotec) and labelled cells were isolated using MS columns (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2020Quote: ... CD3 FITC (Miltenyi Biotec) at room temperature for 20 minutes ...
-
bioRxiv - Immunology 2020Quote: ... CD3 viogreen (Miltenyi Biotec), CD4 PECy7 (BD Pharmingen) ...
-
bioRxiv - Immunology 2020Quote: ... CD3- or CD34-expressing cells by magnetic cell separation (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany).
-
bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells were purified via negative selection using the Naïve CD4+ T cell Isolation mouse Kit (Miltenyi Biotec) according to the manufacturer’s recommendations.
-
bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
-
bioRxiv - Microbiology 2021Quote: ... CD4+ T-cells were first isolated from HLA-DP4+ asymptomatic carriers using the Pan T-cell Isolation Kit (Miltenyi Biotec) and biotin-labeled anti-CD8 mAb (RPA-T8 ...
-
bioRxiv - Immunology 2021Quote: ... Naïve murine CD8+ T cells were isolated using negative selection (Naïve CD8 T Cell Isolation Kit, Cat no. 130-096-543, Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... and T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Naïve antigen presenting cells were isolated from an unimmunized (naïve ...
-
bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
-
bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec) into complete RPMI media ...
-
bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...