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Citations for Miltenyi Biotec :
1 - 50 of 3024 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: 2 × 105 peritoneal exudates cells were stained with fluorochrome-conjugated antibodies (anti-mouse) directed against the T-cell subset surface marker CD4 (Biotin, Miltenyi Biotec), the alpha chain of the IL-2 receptor CD25 (PE ...
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bioRxiv - Immunology 2023Quote: ... to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec) and LS columns (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2022Quote: ... and CD3 antibodies (T-cell activation/expansion kit from Miltenyi Biotec) were combined with isolated rhesus CD4 T cells as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... depleted from CD3+ T cells using CD3 MicroBeads (Miltenyi Biotec) and 1×106 cells were injected intra-hepatically in 30 µl of PBS ...
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bioRxiv - Immunology 2022Quote: ... Total aortic CD3+ T-cells and CD3- cells were isolated using the Pan T cell kit (Miltenyi Biotec).
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of PAN T-Cell Biotin Antibody Cocktail (Miltenyi Biotec) was added and incubated for 5 minutes at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ T cells isolated with Pan T cell isolation kit (Miltenyi Biotec), were stimulated with TransAct Human T cell activation reagent (Miltenyi Biotec) ...
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bioRxiv - Immunology 2019Quote: ... Purified CD4+ T-cells were stained with CD4 antibody (Biotin, Miltenyi Biotec) and CD25 antibody (PE ...
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells were isolated by MACS with CD3 microbeads (Miltenyi Biotec) and labeled with 5 μM CFSE (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells from PBMCs were separated using CD3 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Autologous or allogenic CD3+ T cells used in T cell co-culture experiments were isolated using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instruction.
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bioRxiv - Immunology 2019Quote: ... cells were activated with T Cell TransAct CD3/CD28 (Miltenyi Biotec, Germany) at 1:200 concentration 24h after electroporation and the next day the supernatant was removed to withdraw beads ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... The CD8+ or CD4+ T cells-depleted cell fraction was then depleted from CD3+ cells using CD3 microbeads (Miltenyi Biotec). Cell suspension containing fibrocyte precursors was cultured during at least 14 days to induce fibrocyte differentiation ...
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bioRxiv - Bioengineering 2020Quote: ... CD3 and CD28 monoclonal antibodies (beads T cell activation/Expansion Kit, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) were co-cultured with WJ-MSCs (A/ WJ hMSCs ...
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bioRxiv - Immunology 2022Quote: The CD3+ cells and CD4 T cells were enriched from PBMCs using CD3 MicroBeads (Miltenyi biotec, #130-050-101, German) and CD4 MicroBeads (Miltenyi biotec #130-045-101 ...
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bioRxiv - Cancer Biology 2021Quote: Autologous patient derived T cells were isolated using CD3 MicroBeads (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... or by depletion of T cells with human CD3 MicroBeads (Miltenyi Biotec). NK cells were cultured in SCGM (CellGenix ...
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bioRxiv - Microbiology 2021Quote: ... Non-T-cells containing monocytes and B-cells were prepared by T-cell depletion using anti-biotin MicroBeads (Miltenyi Biotec), biotin-labeled anti-CD4 mAb (OKT4 ...
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bioRxiv - Immunology 2020Quote: ... T cells were activated by injection of anti-CD3/CD28 beads (Miltenyi Biotec) at a ratio of 8:1 beads:T cell followed by injection of oligomycin (2μM ...
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bioRxiv - Biochemistry 2023Quote: ... CD3- T cells representing APC cells were also isolated using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... EGFRt-positive T cells were enriched using biotin-conjugated anti-EGFR mAb and anti-biotin beads (Miltenyi Biotec), and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... CD137-expressing CD4+ memory T cells cells were positively selected by staining with biotin-conjugated CD137 antibody (clone REA765; Miltenyi Biotec) followed by anti-biotin MicroBeads and applied to a new MS column ...
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bioRxiv - Cancer Biology 2022Quote: CD3+ T cells from healthy donors were separated from PBMCs using CD3 immunomagnetic beads (#130-097-043, Miltenyi Biotec, Germany), then amplifing using CD3/CD28 stimulation beads (#11131D ...
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bioRxiv - Immunology 2022Quote: ... Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec, Inc. Cat#130-094-363), CD19 and CD107a (BD Biosciences Cat#555800) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells ...
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bioRxiv - Immunology 2021Quote: ... Cells were restimulated with anti-CD2/anti-CD3/anti-CD28 (T cell activation beads, Miltenyi Biotec) to achieve a 1:1 bead/cell ratio in accordance with the manufacturer’s instructions for cell expansion ...
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bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from the spleen using anti-CD3 magnetic beads from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Cancer Biology 2023Quote: ... The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution; Miltenyi Biotec) in the presence or absence of recombinant mouse TGF-β1 (5 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were then separated from the white cells using mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were isolated using positive selection with mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells (used for in vivo experiments) were supplied with 0.5ng/ml IL15 (Miltenyi Biotec) and 10ng/µl IL7 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T lymphocytes and CD20+ B lymphocytes were sorted from PBMCs using CD3+ and CD20+ microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Microbiology 2021Quote: ... Cell surface staining with anti-CD4 and CXCR4 antibodies (Miltenyi Biotec) confirmed than more than 95% cells were positive for both markers ...
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bioRxiv - Immunology 2020Quote: ... CD3+ T cells were enriched by negative selection on a LD column and QuadroMACS separator (Miltenyi Biotec), before positive selection of γδ T cells using MS columns with an OctoMacs separator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Immunology 2023Quote: ... and stimulated with Dynabeads Human T-Activator CD3/CD28 (Miltenyi Biotec 111.31D) for 48 hours at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: CD3+T-cells were isolated from healthy C57/B6 mice using a Miltenyi kit (Miltenyi Biotec, Auburn, CA). Round bottom plates were coated with 10μg of functional CD3 antibody (eBiosciences ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD3+ T-cells were isolated using negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-095-130). T-cells were then resuspended in RPMI + 10% FBS and 200,000 cells were seeded per tension probe surface ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2021Quote: ... Bone marrows cells were purified with CD3+ T isolated kit (CD3ε MicroBead Kit, mouse, 130-094-973, Miltenyi Biotec). Splenic CD4+ and CD8+ cells were purified in two steps ...
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bioRxiv - Microbiology 2022Quote: CD3+/CD4+ T cells were purified by positive selection using magnetic separation with a CD4+isolation kit (Miltenyi Biotec) using PBMCs from three donors as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Immunology 2019Quote: ... An antibody cocktail designed for negative selection of purified naïve T cells (Naïve Pan T cell purification kit, Miltenyi Biotec) was used together with extra αCD15/CD14 beads ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested from femur and tibiae of 6–9 week-old CD11c-DTR or CD11c-DTR Rag2−/− SJL mice and depleted of T cells using anti-CD3 and anti-CD8 magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). Wild type or Rag2−/− SJL mice were gamma-irradiated with 950 rads from a 60Co source ...
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bioRxiv - Immunology 2021Quote: ... PBMC were then used to isolate autologous CD3+ T cells using the MACS→ (magnetically activated cell sorting) MicroBead Isolation technique (Miltenyi Biotec, Cologne, Germany) according to the manufacturer’s instructions ...