Labshake search
Citations for Miltenyi Biotec :
551 - 600 of 967 citations for Recombinant Human GPC3 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... at a 1:100 dilution and human FcR blocking reagent (Miltenyi Biotec) at a 1:20 dilution on ice for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... and human Treg expansion kit anti-CD3/CD28 beads (Miltenyi Biotec/Invitrogen), using x-vivo 15 cell medium (Lonza ...
-
bioRxiv - Immunology 2024Quote: ... The Human NK Cell Isolation Kit (Miltenyi Biotec; CAT#130-092-657) was used to purify NK cells and the Human Naïve Pan T Cell Isolation kit (Miltenyi Biotec ...
-
bioRxiv - Immunology 2019Quote: ... Cells were washed and labeled for separation by negative selection using a MACS pan-dendritic cell isolation kit (Miltenyi Biotec).
-
bioRxiv - Cell Biology 2020Quote: ... On day 15 of cardiac differentiation fixed cells were labeled for flow cytometry using alpha-actinin (Miltenyi Biotec, Cat.#130106937) and corresponding isotype control ...
-
bioRxiv - Immunology 2023Quote: Splenic CD4+ T cells derived from OT-II TCR-Tg mice were enriched with Biotin-labeled antibody cocktail (B220, CD8a, CD11b, CD11c, CD19, CD25, NK1.1, and TER-119) with Streptavidin Microbeads (Miltenyi Biotec). CD4+ T cells were further incubated with CD62L Microbeads (Miltenyi Biotec ...
-
Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: ... lung single-cell suspensions were labeled with anti-CD4+ or anti-CD8+ magnetic beads and separated by autoMACS (Miltenyi Biotec), or CD4+CD8+ double positive cells purified from thymus single-cell suspensions by flow-cytometric sorting on FACS Aria (BD Biosciences).
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...
-
bioRxiv - Plant Biology 2019Quote: ... Interacting proteins were isolated from total protein extracts using anti-GFP-coupled magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Proteins were digested with trypsin to peptides ...
-
bioRxiv - Genetics 2020Quote: ... depletion of lineage+ cells from MNCs was performed using Biotin labeled Lin+ cocktail and Streptavidin MicroBeads (Macs; Miltenyi Bio-tec) and magnetic columns (Macs; Miltenyi Biotec). For cell sorting of LSK ...
-
bioRxiv - Microbiology 2021Quote: ... HTLV-1-infected cells were sorted as CD4+CADM1+ T-cells using FITC-labeled anti-CADM1 mAb (3E1, MBL) and anti-FITC MicroBeads (Miltenyi Biotec). The CADM1- fractions were used as normal CD4+ T-cells ...
-
bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
-
bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
-
bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
-
bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
-
bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
-
Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: ... Human lung single T cells were prepared from surgical resection and lungs in patients as previously described by selection with biotin-labeled antibodies by autoMACs (Miltenyi Biotec) (Yuan et al. ...
-
bioRxiv - Immunology 2023Quote: ... Red blood cell lysed splenocytes were labeled with microbeads conjugated to monoclonal anti-mouse CD19 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and positively sorted as detailed by the manufacturer ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
-
bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with anti-human CD8-FITC (BW135/80, Miltenyi biotec) and anti-human CD4-PE-Cy7 (SK3 ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 1X human FcR blocker (stock: 50x Mac’s Miltenyi Biotec 130-059-901). After washing ...
-
bioRxiv - Immunology 2021Quote: Human and mouse DCs were stained with the following antibodies from Miltenyi Biotec or as specified ...
-
bioRxiv - Microbiology 2020Quote: ... and monocytes were isolated using human CD14 MicroBeads (130-050-201, Miltenyi Biotec) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Human primary monocytes were isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA) from peripheral blood mononuclear cells obtained from healthy human donors (de-identified ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
-
bioRxiv - Immunology 2020Quote: ... Two-fold dilutions of the anti-human CD303 BDCA2 antibody (AC144) (Miltenyi Biotec), chimeric CBS004 (Capella Bioscience ...
-
bioRxiv - Neuroscience 2022Quote: ... human BDNF (50 µL, 100 ng/mL, MACS Miltenyi Biotec, 130-096-286), LM22A4 (25 µL ...
-
bioRxiv - Cancer Biology 2019Quote: ... an unconjugated mouse anti-human CD133 antibody was used (Miltenyi Biotec; 1:20), and for experiments including FAP ...
-
bioRxiv - Cell Biology 2021Quote: ... Human NP tissue samples were transported in MACS Tissue Storage Solution (Miltenyi Biotec) immediately after surgical resection ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples for surface staining were incubated with human FcR Blocking Reagent (Miltenyi Biotec) together with PE-conjugated mouse anti-GLAST 1:15 (Miltenyi Biotec 130-095-821 ...
-
bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec) and activated with T Cell TransAct (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2021Quote: ... Human CD14 microbeads were used in combination with LS columns (both Miltenyi Biotec) to positively select CD14+ blood monocytes ...
-
bioRxiv - Immunology 2021Quote: ... T cells were selected using human CD4 microbeads and MS columns (Miltenyi Biotec). Procedure was carried out according to manufacturer’s protocol ...
-
bioRxiv - Pathology 2023Quote: ... followed by immunomagnetic separation using a human anti-CD14 purification kit (Miltenyi Biotec). The cell purity was always confirmed by flow cytometry and was superior to 95% ...
-
bioRxiv - Biophysics 2023Quote: ... Monocytes were enriched from human PBMC by CD14 beads positive selection (Miltenyi Biotec) and cultured in IMDM supplanted with 10% human AB serum (omega scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... PBMCs were then labelled with the human NK Cell Isolation Kit (Miltenyi Biotec), with subsequent NK cell isolation using the “depletes” program on an autoMACS Pro Separator and collecting the negative fraction ...
-
bioRxiv - Cancer Biology 2023Quote: ... PE anti-human MSCA1 (Clone W8B2, dilution 1/10, Miltenyi Biotec, Paris, France), APC antihuman CD271 (Clone ME20.4-1.H4 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... T cells were activated with TransAct Human T cell activation reagent (Miltenyi Biotec) and 100 U/ml human IL-2 IS (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 50 IU/ml premium-grade human IL2 (Miltenyi Biotec, 130-097-746) at 1x106 cells per mL and cm2 ...
-
bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... the same method was followed with the substitution of the fluorescent antibody mix against tetraspanins (CD63, CD81 and CD9) with the APC labelled HLA-G antibody (130111852, Miltenyi Biotec, same volume of 15 µl). This method ensured that EVs attached to the Ab conjugated beads were only detected if positive for HLA-G.
-
bioRxiv - Plant Biology 2020Quote: ... and proteins eluted with Elution Buffer (Miltenyi Biotec). Protein extracts were the subjected to immunoblot analyses using α-myc or α-HA antibodies.
-
bioRxiv - Microbiology 2022Quote: ... MultiMACS protein A/G microbeads (Miltenyi Biotec, Germany) were used to catch the secreted proteins as described by[35].
-
bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
-
bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
-
bioRxiv - Cell Biology 2020Quote: ... Human ESCs were maintained in StemMACS™ iPS-Brew XF (iPS-B, Miltenyi Biotec) on Laminin-521 (L521 ...
-
bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Human MSCs were cultured in StemMACS MSC Expansion Medium (Miltenyi Biotec, Bergisch Gladbach, Germany). Murine MSCs and LPR-MSCs were isolated and cultured as previously described (Albarenque et al. ...