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Citations for Miltenyi Biotec :
451 - 500 of 967 citations for Recombinant Human GPC3 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2019Quote: ... The live cell fraction was then incubated with CD45 magnetically-labeled antibody (Miltenyi Biotec, 130-052-301) and passed through a LS magnetic column (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... The diluted blood was labeled with antibodies directed against Ter119 (FITC, TER-119 1/30 Miltenyi Biotec), CD71 (PE ...
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bioRxiv - Immunology 2019Quote: ... CTV-labeled T cells were then isolated using a Pan naïve T cell isolation kit (Miltenyi Biotec) according to manufacturer’s instructions and isolation purity (≥95% ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were depleted of mature hematopoietic cells by anti-mouse antibody-labeled magnetic bead separation (Miltenyi Biotec) before fixation in formalin for 20 min at 4 °C.
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bioRxiv - Immunology 2020Quote: ... pro-B cells were positively selected from bone marrow using anti-CD19-labeled magnetic beads (Miltenyi Biotec) and were cultured for 24h hr in Opti-MEM supplemented with 15% FBS (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: ... The resulting single-cell suspension was labeled with O4-conjugated magnetic MicroBeads (Miltenyi Biotec, 130-096-670) and passed through LS columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Streptavidin-labeled mothers were then captured on LS+ Positive Selection Columns (Miltenyi Biotec, Cat #: 130-042-401). In brief ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... CD14+ cells were isolated from the PBMCs using human CD14+ micro beads conjugated with anti-human CD14 mAbs (130-118-906, Miltenyi Biotec, Bergisch Gladbach, Germany) LC columns (130-042-401 ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... and Vioblue anti-human CD45 antibodies (REA747, #5180719178 Miltenyi Biotec) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... incubated with human and mouse FcR blocking reagents (Miltenyi Biotec) for 15min ...
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bioRxiv - Microbiology 2022Quote: ... PE-Vio770-conjugated anti-human CCR5 (clone REA245, Miltenyi Biotec), PE-conjugated anti-human CD11b (clone REA713 ...
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bioRxiv - Microbiology 2022Quote: ... and CD81 antibodies (Human Exosome Isolation Kit, Pan, Miltenyi Biotec) followed by EV isolation via magnetic separation and elution ...
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bioRxiv - Genetics 2022Quote: ... Then cells were incubated with human CD34 Microbeads (Miltenyi Biotec) and Fc receptor blocker in MACS buffer (1% BSA/2mM EDTA/PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... we used CD45-VioBlue (human antibody, clone REA747, Miltenyi Biotec) to identify immune cells and in few cases ...
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bioRxiv - Genetics 2021Quote: ... Human CD14+ monocytes Isolation kit (Miltenyi Biotec, 130-050-201) and Human CD19+ B Cell Isolation kit (Miltenyi Biotec,130-050-301 ...
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bioRxiv - Molecular Biology 2021Quote: ... PBMCs were incubated with anti-human CD14 MicroBeads (Miltenyi Biotec) and monocytes were separated on a LS magnetic column on a QuadroMACS separator (Miltenyi Biotec) ...
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bioRxiv - Genomics 2021Quote: ... OctoMACS separator and CD19 MicroBeads (human) (all from Miltenyi Biotec) modifying slightly the data sheet protocol according to the experimental needs and the cell types (figure 1).
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bioRxiv - Cancer Biology 2022Quote: ... in FACS buffer containing 1% human Fc Block (Miltenyi Biotec) for 30 mins at 4ºC and were then washed three times ...
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bioRxiv - Systems Biology 2023Quote: ... Monocytes were positively selected using Human CD14 MicroBeads (Miltenyi Biotec) and LS Columns ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-human GLAST (ACSA-1) biotinylated antibody (Miltenyi Biotec, Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 100 U/ml human IL-2 (Miltenyi Biotec) and 100 U/ml Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T Cell TransAct (Miltenyi Biotec t# 130-111-160) was used to activate T cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... after treatment with a human FcR blocking reagent (Miltenyi Biotec). Flow cytometric analysis was performed as described above ...
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bioRxiv - Molecular Biology 2024Quote: A MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) was used ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of human FcR blocking reagent (Miltenyi Biotec) and subsequent staining with anti-PE paramagnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec). Cell purity was verified by flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: Determination of the ROR1 surface expression of T-REx™-293 and its derivates was performed using the anti-ROR1-APC (#130-119-860, Miltenyi Biotec) and Accuri C6 (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Immunology 2019Quote: ... Resuspended cells from the bone marrow were labeled using biotinylated anti-CD3 antibody and streptavidin microbeads (Miltenyi Biotec). Cells were then resuspended in PBS and 1×106 cells were transferred to each mouse.
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bioRxiv - Immunology 2024Quote: ... cells were washed and labeled with CD154-Biotin followed by anti-Biotin MicroBeads (CD154 MicroBead Kit; Miltenyi Biotec) and magnetically enriched by two sequential MS columns (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stained for 10 minutes at room temperature with an intracellular stain cocktail containing the following antibodies: IFN-γ APC and TNF-α PE (all from Miltenyi Biotec). Cells were analyzed on a MACSQuant10 flow cytometer (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: ... were obtained after transduction of Jurkat T cells with a serial dilution of viral supernatants and transduction efficiency evaluated after 3-4 days by monitoring cell surface dNGFR expression using an anti-CD271-APC antibody (Miltenyi Biotec., Paris, France) or HLA-A*02 Dextramer-APC (Immudex ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were enzymatically dissociated using Human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s protocol (https://www.miltenyibiotec.com/AU-en/products/macs-sample-preparation/tissue-dissociation-kits/tumor-dissociation-kit-human.html#gref) ...
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bioRxiv - Neuroscience 2022Quote: ... human BDNF (100 ng/mL, MACS Miltenyi Biotec, 130-096-286), LM22A4 (5 µM ...
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bioRxiv - Immunology 2022Quote: ... using the human CD4+ T cell isolation kit (MACS Miltenyi Biotec). Isolated T helper cell purity was checked by flow cytometry CD4 staining and they were then cultured in CD3 (5µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). Ten micromolar integrase inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng/ml human M-CSF (130-096-491, Miltenyi Biotec), 10 ng/mL GM-CSF (130-093-862 ...
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bioRxiv - Immunology 2020Quote: Human nTregs were CFSE labelled and cultured in TexMACs (Miltenyi Biotec) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Systems Biology 2019Quote: ... antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec) at 4°C for 15 mins ...