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Citations for Miltenyi Biotec :
701 - 750 of 3730 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMCs by three sequential rounds of magnetic separation using CD3 Microbeads (Miltenyi Biotec, 130-050-101), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from splenocytes of OT-I mice on C57BL/6 background using anti-CD8a microbeads (Miltenyi Biotec), and then fluorescently labeled with carboxyfluorescein succinimidyl ester (CFSE) ...
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bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: SB CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Immunology 2023Quote: LV CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16h ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Immunology 2023Quote: ... 20’000 B cells from each condition were cultured for five days with 100’000 CTV-labelled CD4+ T cells along with CytoStim™ (200X dilution, Miltenyi Biotec). TransAct (300X dilution ...
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bioRxiv - Biochemistry 2023Quote: ... and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101, Miltenyi Biotec). CD4+ T cell enrichment was confirmed by determining the percentage of CD3+/CD4+ cells via flow cytometry ...
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bioRxiv - Bioengineering 2024Quote: ... T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec, San Jose, CA). Next ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumors were dissociated using Mouse tumor dissociation kit (Miltenyi Biotec, 130-096-730) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were isolated (Adult Neuron Isolation Kit mouse, # 130-126-603, Miltenyi Biotec) according to the manufacturer’s instructions including a red blood cell removal and a debris removal step (Adult Brain Dissociation Kit P ...
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bioRxiv - Cell Biology 2024Quote: ... The enzyme mix from Neonatal Heart Dissociation Kit mouse and rat (Miltenyi Biotec) was added to the tube and placed into the gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2024Quote: ... The wound tissues underwent enzymatic epidermal-dermal separation using the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) followed by dissociation of the epidermis and dermis parts ...
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bioRxiv - Immunology 2020Quote: ... Spleen cells were incubated at 4°C with anti-CD4 (L3T4)-MicroBeads (Miltenyi Biotec) after 10 min CD4 (RM4-5)-PB ...
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bioRxiv - Microbiology 2023Quote: ... Single cell suspensions were generated by procecessing tissues in gentleMACS C-tubes (Miltenyi Biotec) in a gentleMACS Dissociater (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
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bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
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bioRxiv - Genetics 2022Quote: ... kidneys were quickly dissected after mouse euthanization and transferred to gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in 2ml of digestion buffer (13.5 ml of Dispase (STEMCELL ...
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Developmental Biology 2020Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’ s protocol before staining for FACS sorting was performed.
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bioRxiv - Immunology 2022Quote: ... dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec). Live cells were resuspended in Advanced DMEM/F-12 medium containing 17.51mM D-glucose ...
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bioRxiv - Immunology 2022Quote: ... followed by dead cell removal (Dead Cell Removal Kit, Miltenyi Biotec). NK cells were stained with CellTrace Violet as indicated above ...
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bioRxiv - Cancer Biology 2023Quote: ... Dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed using dead cell removal kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2024Quote: ... dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’s instructions and cell pellet was resuspended in 2% FBS/phosphate-buffered saline ...
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bioRxiv - Cancer Biology 2019Quote: Cytotoxic CD8+ T cells were isolated from spleens of OT-I transgenic mice by magnetic labeling and separation (Miltenyi Biotec, Cambridge, MA). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Immunology 2019Quote: ... The cytoplasmic domain comprised of a truncated CD271(ΔLNGFR) gene for the isolation of T cells expressing CAR was amplified from pMACS-ΔLNGFR (Miltenyi Biotec, Germany) and overlapped with the P2A oligonucleotide ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2021Quote: ... Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec, USA). To obtain high purity Treg ...
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bioRxiv - Biophysics 2023Quote: ... CD4+ and CD8+ T cells were positively sorted from the PBMCs utilizing the StraightFrom® Buffy Coat CD4/CD8 MicroBead (Miltenyi Biotec) and subsequently separated using the QuadroMACS™ Separator (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: Single-cell suspensions from mouse splenocytes were prepared using a cell dissociator (GentleMACS Octo Dissociator, Miltenyi Biotec) based on a previously optimized protocol28 ...
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bioRxiv - Cancer Biology 2021Quote: ... the Lineage Cell Depletion Kit (Miltenyi Biotec) was used to deplete mature hematopoietic cells ...
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bioRxiv - Molecular Biology 2019Quote: ... the Lineage Cell Depletion Kit (Miltenyi Biotec) was used ...
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bioRxiv - Pathology 2021Quote: ... and dead cell removal kit (Miltenyi Biotec) was used to improve cell quality ...
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bioRxiv - Immunology 2021Quote: ... a Dead cell removal kit (Miltenyi Biotec) was used as directed by the vendor.
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bioRxiv - Pathology 2023Quote: ... The Dead Cell Removal Kit (Miltenyi Biotec) was used in accordance with the manufacturer’s instruction to obtain live cells ...
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bioRxiv - Physiology 2020Quote: ... ECs cells were labelled with rat anti-mouse CD31 microbeads (Miltenyi Biotec). Labelled ECs and attached cells were then isolated magnetically ...
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bioRxiv - Immunology 2020Quote: ... Cell suspensions were then processed with Mouse CD45 (TIL) MicroBeads (Miltenyi Biotec) to enrich for CD45+ TILs ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with mouse FcR Blocking Reagent (Miltenyi Biotec, 130092575) for 10 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... following manufacturer instructions (Dead cell removal kit; Debris removal kit; Miltenyi Biotec) resulting in ~80% of purity and CD3+ T cells were resuspended in 1X PBS with 0.04% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... Contaminating mouse splenocytes were depleted from cell preparations by incubating with MACS anti-mouse CD45 magnetic microbeads (Miltenyi Biotec, Auburn, CA) and then washing through magnetic LD Columns as per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumor was dissociated using Mouse tumor dissociation kit (Miltenyi Biotec, 130-096-730) according to manufacturer’s instructions ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... Tumor tissue was minced and dissociated using the mouse Tumor Dissociation Kit (Miltenyi Biotec) and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) ...