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Citations for Miltenyi Biotec :
501 - 550 of 3730 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and subsequently CD4 microbeads for T helper cells (all Miltenyi Biotec, Bergisch Gladbach, Germany). The homogeneity of the cell preparations was controlled by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Cancer Biology 2021Quote: ... the Mouse Cell Depletion KIT was used following the manufacturer’s instructions (Cat# 130-104-694, Miltenyi Biotec).
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bioRxiv - Physiology 2023Quote: ... Hearts were cut in small pieces and dissociated at 37°C with the commercially available enzyme mix (Neonatal Heart Dissociation Kit, mouse and rat, Miltenyi Biotec GmbH; # 130-098-373) followed by tissue homogenization with the genteMACS™ Dissociator (Miltenyi Biotec GmbH ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... PBMCs were stimulated for 3 hours with 10 μg/mL of Gag AK11 peptide and T cells producing IFN-γ in response were enriched using the IFN-γ Secretion Detection and Enrichment Kit (130-054-201; Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were minced using scissors in RPMI media containing enzyme cocktail mix from Miltenyi Biotec mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA). Tumor pieces were further digested as per manufacturer’s instructions and digested tissue was filtered through 70μm strainer to obtain a single cell suspension ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from the spleen using anti-CD3 magnetic beads from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Immunology 2019Quote: ... CD4 T cells were isolated from healthy human donor PBMCs by negative selection (Miltenyi Biotec), and activated with plate-bound anti-CD3 (clone OKT3 ...
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bioRxiv - Genetics 2020Quote: ... and CD4 T cells were positively selected using immunomagnetic microbeads and LS columns (Miltenyi Biotec). Purity was confirmed to be >95% by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated by positive magnetic separation using anti-CD8a microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... adult thymic tissue was depleted of T cells using anti-CD45 MACS beads (Miltenyi Biotec); fetal tissue was not T cell depleted ...
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bioRxiv - Immunology 2019Quote: ... and CD8+ T-cells purified by positive magnetic bead selection (Miltenyi Biotec, 130-117-044) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
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bioRxiv - Microbiology 2021Quote: ... CD4(+) T cells were purified from PBMCs using CD4 MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and the BM was depleted of T cells by autoMACS (Miltenyi Biotec, Bergisch Gladbach, Germany) utilizing CD90.2 microbeads (#130-121-278 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were positively selected with CD4 (L3T4) magnetic microbeads (Miltenyi Biotec, Auburn, CA), pooled and suspended in PBS ...
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bioRxiv - Microbiology 2022Quote: ... CD4(+) T cells were purified from PBMCs using CD4 MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with cardiac troponin T APC-conjugated antibody (Miltenyi Biotec, 130-120-403) or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... CD4+ T cells were isolated from PBMCs by positive selection using CD4+ microbeads (Miltenyi Biotec) or FACS sorting and subsequently expanded through a REP.
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bioRxiv - Cancer Biology 2023Quote: ... The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution; Miltenyi Biotec) in the presence or absence of recombinant mouse TGF-β1 (5 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... naïve T cells were cultured in the presence of IL-6 (30ng/mL; Miltenyi Biotec), soluble anti-CD28 (1µg/mL ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells from PBMCs were separated using CD3 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Naive T cells from spleens and lymph nodes were purified by MACS microbeads (Miltenyi Biotec) and seeded in RPMI-1640 containing 10% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... TNF-alpha (Miltenyi Biotec, Human TNF-α, Cat. No. 130-094-014) IFN-gamma (R&D systems ...
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bioRxiv - Cell Biology 2020Quote: ... Thy1.2 cells were depleted via negative selection using the CD90.2 MicroBeads mouse Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Single cell suspensions from whole mouse lungs were prepared using the lung dissociation kit (Miltenyi Biotec, Auburn, CA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... MuSCs were selected using the Satellite Cell Isolation Kit mouse (130-104-268, Miltenyi Biotec, Bergisch Gladbach, Germany), seeded at 2000 cells/cm2 and grown for 3 days in growth medium (GM ...
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bioRxiv - Cancer Biology 2020Quote: ... MACS-based purification was performed with Myelin Removal Beads II followed by Mouse Cell Depletion kit (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Whole mouse lungs were digested and single cell suspension was prepared using dissociation kit (Miltenyi Biotec, Auburn, CA) as per the vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... GCB cells were first enriched from mouse splenocytes using the PNA MicroBead Kit (Miltenyi Biotec, 130-110-479), followed by antibody incubation and sorting on BD Aria II sorter (RRID:SCR_018091 ...
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bioRxiv - Physiology 2023Quote: Single-cell suspensions were prepared from mouse lungs using the Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... and -Ter119) or c-kit enrichment was performed using MACS magnetic microbead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells ...
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bioRxiv - Immunology 2023Quote: ... and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec) for tumors and lungs respectively ...
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bioRxiv - Immunology 2021Quote: ... the cell suspension was loaded onto an LD column and αβTCR+ T cells were depleted by MACS cell separation per the manufacturer’s protocol (Miltenyi Biotec). After depletion ...
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bioRxiv - Immunology 2022Quote: The CD3+ cells and CD4 T cells were enriched from PBMCs using CD3 MicroBeads (Miltenyi biotec, #130-050-101, German) and CD4 MicroBeads (Miltenyi biotec #130-045-101 ...
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bioRxiv - Immunology 2022Quote: ... cells from lymph nodes and spleens of naïve OT-II or HNT transgenic mice were enriched for naïve cells by percoll gradients and CD4 T cells isolated by CD4 positive selection (Miltenyi Biotec) or using a CD4 naïve positive selection kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... first CD4+ T cells were isolated via positive selection using Magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). APCs were isolated with the same system via CD4 and CD3 negative selection ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were isolated from the spleens of uninfected or MHV-A59 infected animals using the untouched CD8+ T cell isolation MACS kit from Miltenyi Biotec (cat#130-104-075, Gaithersburg, MD) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc receptors were blocked using Fcr Blocking Reagent (Miltenyi Biotec, 130-059-901) according to manufacter’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then negatively selected for CD45 and positively selected for CD117 (c-kit) using magnetic bead separation (Miltenyi Biotec). After selection ...
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bioRxiv - Cancer Biology 2020Quote: ... or CD117/c-kit conjugated with PE (Miltenyi Biotec; RRID: AB_2660118) were used ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...