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Citations for Miltenyi Biotec :
151 - 200 of 881 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... After MACS sorting of CD8+ T cell using a negative selection kit (CD8+ T Cell Isolation Kit II, human, Miltenyi Biotec), cells were lysed for western blotting or stained for Autophagy flux assay as described below ...
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... PolyPeptide group) were co-cultured with 105 OTII CD4+ T cells (purified with a CD4+ T cell isolation kit, Miltenyi Biotec) in RPMI medium (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... OT-1 T cells were isolated from the spleens OT-1 mice using negative selection with CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells obtained using Ficoll density gradient centrifugation (Lymphoprep, ELITechGroup) followed by CD8+ T cell isolation (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec) on buffy coats from HLA-A2.1+ human donors after written informed consent in accordance with the Declaration of Helsinki and in agreement with institutional guidelines ...
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bioRxiv - Immunology 2023Quote: Previously cryopreserved PBMCs were thawed and CD8+ T cells were enriched by negative selection using a nonhuman primate CD8+ T cell isolation kit (Miltenyi Biotec). RNA was isolated from the enriched CD8+ T cells by TRIzol/phenol chloroform extraction with DNase treatment ...
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bioRxiv - Microbiology 2023Quote: ... were gained from EDTA blood by density separation (Lymphocyte Separation Medium (AnproTech)) and CD8+ T-cells were isolated with the human CD8+ T-cell isolation kit (Miltenyi Biotec) and a MidiMACS LS-column according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) or using the EasySepTM Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Synthetic Biology 2023Quote: ... were isolated using Ficoll gradient centrifugation and human T cells were obtained by magnetic sorting using the eHuman Pan T cell isolation kit (Miltenyi Biotec). Once isolated ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from wildtype mice spleens using CD8a+ T Cell Isolation Kit (130-104-075) from Miltenyi Biotec according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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bioRxiv - Immunology 2024Quote: Single cells suspension from tumour-bearing lungs described previously were used for CD8+ T cells sorting using immunomagnetic sorting using CD8α+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... OT-I CD8 T (OT-I) cells were enriched by negative selection using a CD8a+ T cell isolation kit (Miltenyi Biotec), commonly reaching a purity of 90-98% as determined by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the minced auricular lymph nodes using the CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... Autologous CD4+ T cells were isolated from PBMCs with a negative CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: Splenocytes or small intestinal IELs isolated from QFLTg or OT-1 mice were depleted of non-T cells using Pan T isolation Kit II (Miltenyi Biotec). The negatively selected T cells were then labeled with 5µM Cell Tracing Violet (CTV ...
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bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were magnetically purified using CD4+ T cell isolation kit and the autoMACS® Columns Pro Separator machine (Miltenyi Biotec), according to the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were separated from healthy controls PBMC using pan T cell isolation kit and magnetic-activated cell sorting (Miltenyi Biotec, USA). Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from splenocytes either by using the CD4+ or CD8+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s recommendation alone or followed by anti-CD4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T cells were magnetically separated by negative selection with a Human Pan T cell isolation Kit (#130-096-535, Miltenyi Biotec, Germany) and stored long-term in Bambanker serum-free cell freezing medium (#BB01 ...
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bioRxiv - Immunology 2023Quote: ... Mouse CD4+ T cells were purified by negative selection using a CD4+ T cell isolation kit (Miltenyi Biotec, cat. # 130-104-454). For CD8 depletion ...
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bioRxiv - Physiology 2023Quote: ... CD4+ T cells were then purified from the isolated PBMCs by negative selection using humanCD4+ T cell isolation kits (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: CD8+ T cells were isolated by MACS (Miltenyi Biotec) from draining inguinal lymph nodes of MB49 bearing male and female mice 14 days post implantation (Fig ...
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bioRxiv - Immunology 2022Quote: ... using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were negatively selected by MACS (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T Cell Isolation Kit was from Miltenyi Biotec Ltd. ...
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bioRxiv - Immunology 2021Quote: ... or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec), stimulated with Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated by MACs (Miltenyi Biotec) from the spleens of naïve mice ...
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bioRxiv - Immunology 2023Quote: ... T cells were depleted using CD90.2 microbeads (Miltenyi Biotec). Mice were maintained on 0.03% enrofloxacin in drinking water for up to 1 wk before and for 2 wk after irradiation and then were housed in autoclaved cages with sterile water ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells positively sorted by MACS (Miltenyi Biotec) were assessed for viability by AO/PI staining and counted with a Luna-FX7TM Automated cell counter (Logos Biosystems).
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bioRxiv - Microbiology 2024Quote: ... using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and were cultured in RPMI medium containing 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Freshly tumor (n = 8) and adjacent normal lung samples (n = 8) were delivered within MACS® Tissue Storage Solution (Miltenyi Biotec) on ice directly from the operating room to the laboratory after frozen pathology (1-2h).
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bioRxiv - Immunology 2019Quote: ... Mouse total T and CD8+ native T cells were enriched from spleens and lymph nodes by negative selection using MACS system (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... T cells were magnetically separated from other splenocytes using the Mouse Pan-T Cell Isolation Kit II (Miltenyi Biotec, Cat# 130-095-130), labeled with CellTrace™ CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... M and N protein were purchased from Miltenyi Biotec. Domains of SARS-CoV-2 S protein showed in Figure S2A was previously described (Wrapp et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in MACS-buffer und T cells were MACS-sorted from suspension using a Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533). Per 1 × 107 total cells ...
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bioRxiv - Bioengineering 2021Quote: ... CD3/CD28-mediated activation of T cells was conducted by using 10 μl of T cell TransActTM (130-111-160; Miltenyi Biotec B.V. & Co. KG) according to manufacturer’s instructions (T cell activation medium) ...
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bioRxiv - Immunology 2020Quote: ... OT-I CD8+ T cells were then purified by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec: 130-104-075). For cell division or microscopy studies ...