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Citations for Miltenyi Biotec :
401 - 450 of 881 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated and expanded with the T Cell Activation/Expansion Kit (Miltenyi Biotec, Cat No. 130-091-441) according to the vendor’s manual ...
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bioRxiv - Biochemistry 2023Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... the enriched CD8+ T cells were stimulated with the CMVpp65 overlapping peptide pool PepTivator® CMVpp65 (Miltenyi Biotec) at a concentration of 1 µg of each peptide/ml for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ T cells were purified using a magnetic bead separation kit (Miltenyi Biotec, Cat #130-104-075). Rag1-/- mice were reconstituted with CD8+ T cells by intravenous route (3 x 106 cells/mouse ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T lymphocytes and CD20+ B lymphocytes were sorted from PBMCs using CD3+ and CD20+ microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were then separated from the white cells using mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Purity of CD4+ T cell population was measured by flow cytometry (MACSquant Analyzer using MACSQuantify software, Miltenyi Biotec), showing a fraction of at least 95% CD4+CD3+ double positive cells and less than 0.01% CD123+CD304+CD303+ cells ...
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bioRxiv - Microbiology 2024Quote: Primary CD4+ T cells were purified from PBMCs of a healthy donor by negative selection (Miltenyi Biotec, USA). Purified CD4+ T cells were stimulated with 1 µg/mL soluble anti-CD3 (clone OKT3 ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD3+ T-cells were isolated using negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-095-130). T-cells were then resuspended in RPMI + 10% FBS and 200,000 cells were seeded per tension probe surface ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Microbiology 2023Quote: ... penicillin (100 U/ml) and interleukin 2 (IL-2, Miltenyi Biotec #130-097-745) (10 ng/ml) ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant was incubated with 50 L magnetic mMACS anti-DYKDDDDK Microbeads (Miltenyi Biotec) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... Bone marrows cells were purified with CD3+ T isolated kit (CD3ε MicroBead Kit, mouse, 130-094-973, Miltenyi Biotec). Splenic CD4+ and CD8+ cells were purified in two steps ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD8+ T cells were purified from the spleen of OT-I.Ly5.1 mice using CD8a+ T Cell Isolation Kit II (Miltenyi Biotec). OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were enriched from spleen with CD4 microbeads (Clone: L3-T4, Miltenyi Biotec, San Diego, CA, USA). The enriched CD4+ T cells were stained with antibodies to define TFH cells as described in flow methods below ...
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bioRxiv - Immunology 2021Quote: Primary murine naïve CD4 T cells were isolated from the spleens and lymph nodes of SLE1.2.3 or control mice using the CD4+CD62L+ T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). CD62L positive selection was routinely ∼98% in purity as determined by flow cytometry ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens by using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... CD8+ T cells were isolated by negative selection using magnetic beads following the instructions from the manufacturer (Miltenyi Biotec). The purified CD8+ T cells were incubated with APC-conjugated HLA-A2/YFV NS4b (LLWNGPMAV ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Germany), resuspended at 106 cells/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Positive selection was performed to isolate CD8+ T cells from primary tumors and spleens using CD8a microbeads (Miltenyi Biotec), an LS column ...
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bioRxiv - Immunology 2021Quote: ... Cells were negatively enriched for CD8 cells using the Miltenyi Biotec mouse CD8+ T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQUANT Analyzer (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQuant Analyzer (Miltenyi Biotec).
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bioRxiv - Microbiology 2022Quote: CD3+/CD4+ T cells were purified by positive selection using magnetic separation with a CD4+isolation kit (Miltenyi Biotec) using PBMCs from three donors as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: hCD8+ T cells were isolated from commercially available sources (buffy coats from ZenBio Inc. and leukopaks from Miltenyi Biotec) using magnetic separation (positive selection ...
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from spleens and non-pancreatic draining lymph nodes (ndLN) by MACS enrichment (Miltenyi Biotec). Treg and Teff cells were then sorted from CD4+ T cells (Teff ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Immunology 2023Quote: SIY-reactive CD8+ T cells were isolated from the spleen and lymph nodes from TCR-transgenic 2C CD45.1+ or 2C CD45.2+ Rag2-/- mice using the CD8a+ T cell isolation kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM CHIR99021 (Miltenyi Biotec), 0.5 μM A83-01 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μM Purmorphamine (Miltenyi Biotec), 100 ng/ml FGF8 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec) and TNF-α-APC (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 ng/mL Fibroblast Growth Factor 2 (FGF-2) (Miltenyi Biotec, 130-105-787), for 14 days with medium change every 2 days ...
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bioRxiv - Microbiology 2024Quote: ... Splenocytes were stimulated with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
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bioRxiv - Immunology 2021Quote: ... The uncollected non-myeloid (CD11b-) cells were processed with the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-095-130) following the manufacturer’s protocol ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Human CD4+ T cells were isolated by positive magnetic selection using human CD4 MicroBeads kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified with either anti-CD8 or anti-CD4 magnetic beads using MACS columns (Miltenyi Biotec, Auburn, CA) prior to cell surface staining ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ and CD8+ T cells were isolated by MACS from monocyte-depleted PBMC using CD4 and CD8 microbeads (Miltenyi Biotec). These primary cells were cultured in X-vivo 15 (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
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bioRxiv - Immunology 2022Quote: ... total CD4+ T cells were directly isolated from buffy coats using the StraightFrom Buffy Coat CD4 MicroBead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... PMEL T cells were isolated from the splenocytes of the Thy1.1+ PMEL TCR transgenic mice by CD8a+ negative selection (Miltenyi Biotec). Each irradiated Krt14-Kitl* mouse received 1×106 cells of PMEL plus 1.8×106 cells of the peptide-pulsed MHC-II+ ...
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bioRxiv - Bioengineering 2022Quote: ... for the cardiac marker Cardiac troponin T conjugated to APC (cTnT; 1:50; REA400; 130-120-543, Miltenyi Biotec, Germany), and cells were frozen in RPMI + B27C with 10 µM of RI + 10% FCS + 10% DMSO ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... then resuspended in PBE containing FITC-conjugated antibody specific for cardiac troponin T (Miltenyi Biotec, cat no. 130-119-575) at a concentration of 1:50 and incubated for 2 h at 4°C ...