Labshake search
Citations for Miltenyi Biotec :
401 - 450 of 1108 citations for 6 Hydrazino 1 2 4 triazolo 4 3 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... and 3 ng/ml IL-15 (#130-093-955, Miltenyi Biotec).
-
bioRxiv - Neuroscience 2024Quote: ... and 20 ng/mL NT-3 (Miltenyi Biotec, #130-093-973) from day 34 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The purity of isolated B cells was over 95% as assessed by flow cytometry analysis of PE-CD19+ cells (Miltenyi Biotec) 87,88.
-
bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
-
bioRxiv - Immunology 2021Quote: ... Samples were then transferred to 15 mL conical tubes and non-memory B cells were depleted using the AutoMACS Magnetic Sorter (Miltenyi Biotec) “deplete” program ...
-
bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
-
bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells, #130-092-657, Miltenyi Biotec for natural killer cells ...
-
bioRxiv - Immunology 2023Quote: ... B cells were first enriched from the splenocyte suspension by MACS (magnetic activated cell sorting) using the Pan B cell Isolation Kit (Miltenyi Biotec), followed by staining for FACS (fluorescence activated cell sorting) ...
-
bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... staining and single-cell sorting of PvAMA1-specific IgG+ MBCs were performed as follows: B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec) and stained with mouse anti-human CD20 (PE-Cy5.5 ...
-
bioRxiv - Neuroscience 2024Quote: 10 μl of anti-ACSA-2 MicroBeads (Anti-ACSA-2 MicroBead Kit, # 130-097-678, Miltenyi Biotec) and an additional incubation for 30 min ...
-
bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
-
bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
-
bioRxiv - Biochemistry 2021Quote: Memory B cells were isolated from Peripheral blood mononuclear cells (PBMCs) derived from blood samples from COVID-19 convalescent patients using a Human Memory B cell isolation kit (Miltenyi Biotec, GE). Small pools of purified Memory B cells were seeded into 384-well plates on irradiated CD40L-expressing feeder cells for differentiation into plasma cells as described previously (41) ...
-
bioRxiv - Immunology 2022Quote: ... B cells were isolated using either the StraightFrom™ Buffy Coat CD19 Microbead Kit or the Human B cell Isolation Kit II (both from Miltenyi Biotec), following the manufacturer’s protocols ...
-
bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
-
bioRxiv - Cancer Biology 2023Quote: Splenic B cells from mice immunized with SRBC for 10 days were pre-enriched by B220 MicroBeads (Miltenyi Biotec; 130-049-501) followed by flow sorting ...
-
bioRxiv - Immunology 2020Quote: ... PBMCs were stimulated with SARS-CoV-2 peptide pools of S and N (2 μg/ml) (Miltenyi Biotec) in the presence or absence of CECs ...
-
bioRxiv - Cell Biology 2019Quote: ... Interleukin 3 (IL3) was added (1ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany) to EM on the first day (stage I) ...
-
bioRxiv - Immunology 2022Quote: ... The library was selected for 3 rounds with streptavidin beads (Miltenyi Biotec) loaded with biotinylated TCR and then for 1-2 additional rounds with tetramers of TCR and anti-AlexaFluor647 microbeads (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2022Quote: ... (3) Chondrogenic medium: StemMACSTM ChondroDiff Medium from Miltenyi Biotec (Bergisch Gladbach, Germany). MSCs were cultured in 12-well plates ...
-
Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 12 ng/ml FGF-2 (Miltenyi Biotec) and 20 ng/ml Activin A (PeproTec ...
-
bioRxiv - Cancer Biology 2023Quote: ... + 2% BSA (Miltenyi Biotec: 130-091-376) + 0.2 U/μL RNase inhibitor ...
-
bioRxiv - Microbiology 2023Quote: ... IL-2 (Miltenyi Biotec, 130-097-745) was supplemented (100 unit/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
-
bioRxiv - Microbiology 2023Quote: Approximately half of the lung and the left hemisphere of each brain were weighed and homogenized in 1 mL of DMEM supplemented with 2% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
-
bioRxiv - Microbiology 2022Quote: PBMCs isolated from healthy donors (n=6) were used for CD4+T cell isolation kit (Miltenyi Biotec). CD4+T cells were treated with recombinant IL-2 ...
-
bioRxiv - Microbiology 2022Quote: ... mature parasite cultures (6-8% parasitemia) were magnetically separated using magnetic columns (MACS LD columns, Miltenyi Biotec) and diluted to 1% parasitemia containing 0.5 mL fresh erythrocytes88 ...
-
bioRxiv - Immunology 2022Quote: CD8+ T cells were positively sorted with beads from the spleens of C57BL/6 mice (Miltenyi Biotec). The T cells were labelled with CFSE (Invitrogen Molecular Probes) ...
-
bioRxiv - Immunology 2023Quote: ... moDCs were supplemented with a maturation cocktail: IL-6 (15 ng/ml, #130-093-933, Miltenyi Biotec), TNF-α (10 ng/mg ...
-
bioRxiv - Immunology 2024Quote: ... DCs were purified (>90%) from the spleens of C57BL/6 mice using MACS CD11c microbeads (Miltenyi Biotec). Freshly isolated CD8+ T cells were then activated either with anti-CD3 and anti-CD28 antibody-coated microbeads at a 1:1 bead:cell ratio (Miltenyi Biotec ...
-
bioRxiv - Immunology 2021Quote: B cells from spleens and lymph node of naïve mice were purified by magnetic separation using a pan-B cell isolation kit (Miltenyi Biotec #130-095-813), then incubated overnight with 30 μg/ml LPS (Sigma #L2630 ...
-
bioRxiv - Immunology 2022Quote: ... B cells were depleted from OTII splenocytes by magnetic sorting using CD45R (B220) MicroBeads according manufacturer’s instructions (Miltenyi Biotec cat. 130-049-501). For experiments involving cell division analysis ...
-
bioRxiv - Bioengineering 2022Quote: Omicron RBD-specific memory B cells were isolated from pre-enriched memory B cells by magnetic positive selection according to the manufacturer’s protocol (Miltenyi Biotec, 130-095-838). Briefly ...
-
bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
-
bioRxiv - Systems Biology 2022Quote: ... and T and B cells were depleted by using CD19 and CD3 MicroBeads (Miltenyi Biotec Cat#130-097-055 and #130-097-043) to enrich for myeloid cells ...
-
bioRxiv - Immunology 2022Quote: B cells from PBMCs of uninfected controls or of COVID-19 convalescent individuals were enriched using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
-
bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells ...
-
bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
-
bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
-
bioRxiv - Developmental Biology 2019Quote: ... NeutrAvidin-functionalized devices were incubated with either (1) 2 μg/ml biotinylated mouse anti-human anti-SSEA-5 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) (2 ...
-
bioRxiv - Immunology 2021Quote: ... 100 ng/ml of CD3-specific antibody (OKT-3; Miltenyi Biotec, Gladbach, Germany), and cultured in tissue culture flasks (TPP ...
-
bioRxiv - Immunology 2023Quote: ... injected with 3 μg of anti-mouse CD45-FITC antibodies (Miltenyi Biotec, Germany) retro-orbitally and left for 3 min prior to euthanasia by cardiac puncture ...
-
bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with MACSima™ Running Buffer (Miltenyi Biotec, 130-121-565) before nuclear staining was performed with DAPI (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2024Quote: ... After 3 washes with Auto-MACS rinsing solution (Miltenyi Biotec #130-091-222) containing 0.5% BSA (Miltenyi Biotech #130-091-376) ...
-
bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... or the PepTivator SARS-CoV-2 Prot_N peptide mix (covering the complete sequence of the SARS-CoV-2 N protein; both Miltenyi Biotec). As controls splenocytes were either left unstimulated or activated polyclonally with 12.5 µg/mL phytohemagglutinin (PHA) ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were resuspended in 8 ml cold PBE buffer (PBS with 0.5% BSA and 2 mM EDTA) and 2 ml anti-PE microbeads (Miltenyi Biotec) per 108 cells and incubated for 15 minutes at 4 °C ...