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Citations for Miltenyi Biotec :
301 - 350 of 1172 citations for 6 Hydrazino 1 2 4 triazolo 4 3 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... cell pellets were resuspended in 1 mL FACS buffer (DPBS with 2% BSA, Miltenyi Biotec #130-091-376 ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Immunology 2022Quote: ... Naïve B cells were then purified by negative selection using anti-CD43 microbeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by B cell enrichment using anti-CD19 magnetic beads (Miltenyi Biotec, 130-050-301) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Magnetic columns were used to deplete non-B cells according to standard protocol (Miltenyi Biotec). Cells in effluent were further labeled with NP-PE ...
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bioRxiv - Immunology 2021Quote: ... we purified B cells (Fig. 1D) by positive selection using magnetic beads (MACS, Miltenyi Biotec) conjugated with a monoclonal anti-mouse B220 antibody (clone ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were isolated from 16 healthy individuals and enriched for CD19+ B cells (Miltenyi Biotec). Two times 2.5 × 105 of naive B cells (CD20+IgM+IgD+IgG− ...
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bioRxiv - Immunology 2021Quote: ... B cells were excluded from whole splenocytes by incubating with anti-CD19 beads (Miltenyi Biotec) or by using the EasySep™ mouse CD19 positive selection kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: B cells were isolated from cryopreserved PBMCs by positive selection using CD19+ microbeads (Miltenyi Biotec) and expanded through CD40-CD40L stimulation by culturing cells for 4-5 days with irradiated NIH3T3 feeder cells constitutively expressing CD40L at 37°C in 5% CO2 in B cell medium ...
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bioRxiv - Immunology 2023Quote: ... The cells were subsequently processed using the Pan B Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Molecular Biology 2024Quote: ... MDSL and TF-1 were additionally supplemented with 25ng/mL of recombinant human IL-3 (Miltenyi Biotec, 130-095-069), and MDS-L with 50nM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 25 ng/ml IL-3 (Miltenyi Biotec), 2 mM Glutamax (Invitrogen Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... 25 ng/ml IL-3 (Miltenyi Biotec), 100 ng/ml M-CSF (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2020Quote: ... ~100 μg Fe / 1 × 106 cells (40 μl Miltenyi Biotec FeraTrack SPIO / 2 ml culture medium) was chosen according to cell viability and manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Immunology 2024Quote: ... OT-1 or Thy1.1 OT1 cells were purified via CD8a (Ly-2) MACS microbeads (Miltenyi Biotec), unless otherwise indicated ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Isolated cells were incubated with fluorochrome conjugated antibodies ACSA-2-PE (1:50; Miltenyi Biotec, REA969), A2B5-488 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Anti-IL-10 (REA842) and anti-IL-6 (REA1037, Miltenyi Biotec) were used for intracellular staining.
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bioRxiv - Microbiology 2021Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to electroporation.
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bioRxiv - Microbiology 2022Quote: ... and 50 U/mL interleukin-2 (IL-2, Miltenyi Biotec) prior to infection.
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bioRxiv - Immunology 2019Quote: Splenic CD4+ T cells or CD19+ B cells were positively selected using immunomagnetic MicroBeads (Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec). Cells were washed twice with 5 ml FACS buffer with 3 mM EDTA and adjusted to a cell density of 1-2×106 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified with CD43 micro beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany), and 1×106 cells were placed in the top chamber of a transwell plate in 100μL of volume ...
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bioRxiv - Microbiology 2021Quote: ... and B-cells were affinity-purified using CD19 microbeads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) resulting in >95% pure B-cell populations ...
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bioRxiv - Immunology 2022Quote: ... B cells were first isolated from donors’ PBMC by MACS using human CD19 MicroBeads (Miltenyi Biotec). CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes ...
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bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2020Quote: ... was added to B cells and then analyzed by flow cytometry (MacsQuant Analyzer 10; Miltenyi Biotec,). The double labeling procedure allows a distinction into living (Annexin V −/PI−) ...
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bioRxiv - Microbiology 2024Quote: CD19+ B cells were purified by positive magnetic separation using CD19 MicroBeads (Miltenyi Biotec, Gaithersburg, MD) and MS or LS columns ...
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bioRxiv - Immunology 2022Quote: ... using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Cell Biology 2024Quote: ... and then pelleted prior to resuspension in MB containing anti-mouse IgG2a+b MicroBeads (Miltenyi Biotec) and incubation for 15 min at 4°C ...
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). Ten micromolar integrase inhibitor ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). All HTLV-1 infected T-cell clones were maintained in 10 µM of the integrase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Genomics 2020Quote: ... and passed through 3 LS Columns (Miltenyi Biotec). Mouse embryonic stem cells (ESC ...
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bioRxiv - Microbiology 2019Quote: ... CD19 microbeads were used for magnetic separation of purified B cells using an autoMACS separator (Miltenyi Biotec). All cell lines in this study were cultured according to standard mammalian tissue culture protocols (ATCC ...
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bioRxiv - Immunology 2021Quote: ... CD19+ B cells were purified from human PBMCs using the REAlease® CD19 Microbead Kit (Miltenyi Biotec) according to the manufacturer’s recommendations with purity greater than 98 %.
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bioRxiv - Immunology 2019Quote: ... untouched B cells were enriched using anti-CD43 magnetic beads (MACS) according to manufacturer’s protocol (Miltenyi Biotec) obtaining > 95 % purity ...
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bioRxiv - Immunology 2020Quote: ... pro-B cells were positively selected from bone marrow using anti-CD19-labeled magnetic beads (Miltenyi Biotec) and were cultured for 24h hr in Opti-MEM supplemented with 15% FBS (Life Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were neutralized with Ca2+/Mg2+ free DPBS with 0.5% BSA (Miltenyi Biotec, 130-091-376) and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... B lymphocytes were enriched from erythrocyte-lysed spleen cells using CD43 MicroBeads (Miltenyi Biotec,130-049-801) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: B cells were purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec) and subsequently expanded for 10-14 days in RPMI-1640 supplemented with 10% human serum (Bavarian Red Cross) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike peptides (PepTivator SARS-CoV-2 Prot_S, Miltenyi Biotec) were added at a final concentration of 0.6 nmol/ml in the presence of 1 μ (total cell culture volume ...
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bioRxiv - Cancer Biology 2022Quote: ... and then blocked in FACS buffer (PBS, 2% BSA) containing FcR blocking reagent at 1:50 (Miltenyi Biotec) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...