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Citations for Miltenyi Biotec :
4051 - 4100 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... BAT was dissociated using a gentleMACSTM Dissociator (Miltenyi biotec, Germany). The cell suspension was cross linked with 1 % formaldehyde for 10 min at RT and the reaction was quenched with 0.125 M glycine for 5 min to 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... Lamina propria fractions were prepared by enzymatic digestion and dissociation with the Lamina Propria Dissociation Kit and GentleMacs Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Dissociated cells were collected for use in qPCR analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
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bioRxiv - Cancer Biology 2022Quote: ... and then blocked in FACS buffer (PBS, 2% BSA) containing FcR blocking reagent at 1:50 (Miltenyi Biotec) for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... the solution was passed over a 30 µm cell filter (Miltenyi Biotec, 130-041-407). To remove dead cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Muscle stem cells (MuSCs) were isolated from mouse TA muscle using a satellite cell isolation kit (Miltenyi Biotec) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... and 25 ng/ml macrophage colony-stimulating factor (M-CSF; Miltenyi Biotec). HeLa and HEK 293 FT cells were maintained by bi-weekly subpassage in DMEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... and monocytes were isolated by positive selection using CD14+ magnetic beads (Miltenyi Biotec) and differentiated into macrophages for 7 days in RPMI (Gibco ...
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bioRxiv - Immunology 2022Quote: ... CD45+ cells (immune cells) and CD31+ (endothelial cells) were then depleted using mouse CD45 and CD31 MicroBeads (Miltenyi Biotec) and the remaining cells were counted and incubated with anti-CD326-APC (eBioscience) ...
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bioRxiv - Cell Biology 2022Quote: Neonatal cardiomyocytes were purified by immunomagnetic separation using the Neonatal Cardiomyocyte Isolation Kit (Miltenyi Biotec), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... CD14+ monocytes were isolated from PBMCs using CD14 MicroBeads (Miltenyi Biotec) and subsequently differentiated into MoDCs or MoLCs ...
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bioRxiv - Microbiology 2022Quote: ... both from Miltenyi Biotec (Cytobox Mo-DC). MoLCs were generated as MoDCs ...
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bioRxiv - Microbiology 2022Quote: ... recombinant anti-DC-SIGN (clone REA617, Miltenyi Biotec), mouse anti-CD1a (Novus Biologicals) ...
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bioRxiv - Microbiology 2022Quote: ... Migratory cells were either used directly for experiments or subjected to a positive selection using CD1a Microbeads (Miltenyi Biotec), in order to enrich epidermal Langerhans cells ...
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bioRxiv - Microbiology 2022Quote: ... FITC-conjugated anti-HLA-DR (clone AC122, Miltenyi Biotec), mouse anti-HSP90 (clone F-8 ...
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bioRxiv - Bioengineering 2022Quote: ... and residual red blood cells were lysed using MACSxpress Erythrocyte Depletion Kit (Miltenyi Biotec). Blood was drawn from a total of three nondiseased donors with informed consent obtained at the time of the blood draw according to requirements of the IRB ...
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bioRxiv - Bioengineering 2022Quote: ... Neutrophils were purified from whole blood using the MACSxpress Neutrophil Isolation Kit per the manufacturer’s instructions (Miltenyi Biotec) and residual red blood cells were lysed using MACSxpress Erythrocyte Depletion Kit (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2022Quote: ... CD34+ haematopoietic stem and progenitor cells (HSPCs) were purified using CD34+ Microbead kit (Miltenyi Biotec, UK) according to the manufacturer protocol ...
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bioRxiv - Bioengineering 2022Quote: Harvested tumors were weighed and homogenized using a gentleMACS tissue dissociator (Miltenyi Biotec) (C-tubes ...
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Understanding immune-driven brain aging by human brain organoid microphysiological analysis platformbioRxiv - Bioengineering 2022Quote: ... Monocytes were isolated from PBMCs using Classical Monocyte Isolation Kit according to manufacturer’s protocol (Miltenyi Biotec GmbH) with MS Column (Miltenyi Biotec GmbH) ...
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Understanding immune-driven brain aging by human brain organoid microphysiological analysis platformbioRxiv - Bioengineering 2022Quote: ... with MS Column (Miltenyi Biotec GmbH). Typically ...
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bioRxiv - Bioengineering 2022Quote: ... and negative immunomagnetic bead selection according to the manufacturer’s protocol (Miltenyi Biotec, Germany). Primary lymphocytes were stimulated with CD3/CD28 T-cell activation Dynabeads (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... and the differentiation status was validated using flow cytometry (CD11b, Miltenyi Biotec). Before experimentation ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then trypsinized and incubated with anti-CD146 antibody coated microbeads (Miltenyi Biotec Inc.) for 15 mins at 4°C to obtain the CD140b+CD146+ cells for subsequent experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Purified stromal cells were then separated from epithelial cells using anti-CD326 (EpCAM) antibody-coated microbeads (Miltenyi Biotec Inc., San Diego, CA, USA). Next ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subjected to negative selection using CD45 magnetic microbeads (Miltenyi Biotec # 130-052-301). Fibroblasts cells were identified as CD45- CD31- EpCAM- PDGFR+ by flow cytometry as outlined in Fig ...
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bioRxiv - Cell Biology 2022Quote: ... and coarsely minced before processing in C-tubes (Miltenyi Biotec) with a GentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... with a GentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with anti-cytochrome c-APC antibody (Clone#REA702, 1:50) (Cat#130111180, Miltenyi Biotec). Samples were analyzed on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... and mitochondria were isolated and enriched using a Mitochondria isolation kit as the manufacturer’s introductions (Cat#130096946, Miltenyi Biotec). HeLa cells were harvested and permeabilized by 0.025% w/v digitonin (Biosynth ...
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bioRxiv - Developmental Biology 2022Quote: ... then bipotent progenitors were positively selected using a biotinylated EpCAM antibody (clone G8.8, eBiosciences) and streptavidin-conjugated magnetic beads (Miltenyi Biotec). This generates a >95% pure preparation of e16.5 bipotent alveolar progenitors (Sftpc+ Rage+ cells) ...
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bioRxiv - Developmental Biology 2022Quote: ... bipotent progenitors were isolated by MACS using MS columns (Miltenyi Biotec) according to the vendor protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... CD45 and Pdgfrα (Miltenyi Biotec), then bipotent progenitors were positively selected using a biotinylated EpCAM antibody (clone G8.8 ...
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bioRxiv - Immunology 2022Quote: ... Hepatic lymphocytes were isolated using Liver dissociation kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Mononucleated cells from PB or BM were then enriched for CD34+ cells on the AutoMacs system using human CD34 MicroBead kit (Miltenyi Biotec, #130-046-702) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining (1 ×106 PBMC) was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Immunology 2022Quote: ... magnetic anti-biotin beads (Miltenyi Biotec) were added and CD4+ T cells were positively selected by running cells along a MACS magnet ...
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bioRxiv - Immunology 2022Quote: ... Naive CD4+ T cells were purified from spleens of OT II mice by MACS (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... pelleted splenocytes isolated from mice spleen were resuspended in FACS for NK cells purification with Mouse NK Cell Purification Kit (Miltenyi Biotec) according to the ...
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bioRxiv - Immunology 2022Quote: ... CD27+ CD8 T cells were separated with CD27 MicroBeads (Miltenyi Biotec). Negatively selected population were incubated with CD45RO MicroBeads for second round of separation on CD27-CD45RO+ and CD27-CD45RO-CD8 T cell subsets ...
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bioRxiv - Immunology 2022Quote: CD8 T cells were purified from PBMC by negative immunomagentic sorting using CD8+ T Cell Isolation Kit (Miltenyi Biotec) and used for bilayers experiments or for further separation into the subsets ...
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bioRxiv - Immunology 2022Quote: ... Mouse hepatic parenchymal cells were digested and non-parenchymal cells were isolated by Mouse Liver Dissociation Kit (Miltenyi Biotec). Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
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bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
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bioRxiv - Cell Biology 2022Quote: ... CD45-cell fraction was collected using the MultiMACS Cell24 Separator (Miltenyi Biotec). Cells were sorted as described above ...
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bioRxiv - Cell Biology 2022Quote: ... using GentleMACS/OctoMACS dissociator (Miltenyi Biotec). Suspensions were filtered through 40 μM strainer and rinsed with MACS buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Microbeads were separated from flow-through by running through μColumns (MACS, Miltenyi Biotec, 130-042-701) and three washes with RIPA buffer ...