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Citations for Miltenyi Biotec :
3851 - 3900 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the caudate and median liver lobes were harvested in RPMI-1640 medium (Biowest Riverside, MO, US) and mechanically minced using the gentleMACS Dissociator (Miltenyi Biotec, Auburn, CA, USA). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... and further processed into dead-cell depleted single cell suspensions following manufacturer’s protocol using Tumor Dissociation Kit and MACS Dead Cell removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... in 300 µL of total media and placed on GentleMACS dissociator (Miltenyi Biotec, Cat# 130-095-235) for 15 mins (2x after 10 mins interval) ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was incubated with RBC lysis buffer for 5 min on ice to remove red blood cells (RBC) and then used for Treg isolation with a CD4+CD25+ Treg isolation kit (Miltenyi Biotec, cat # 130-091-041), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... CD4 T cells were enriched on autoMACS Pro Separator (Miltenyi Biotec) with CD4 positive selection beads (clone L3T4 ...
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bioRxiv - Cancer Biology 2023Quote: ... with CD4 positive selection beads (clone L3T4, Order no. 130-117-043, Miltenyi Biotec), followed by FACSorted on a Moflo Astrios (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... Granzyme B (1:75, PE; Miltenyi Biotec #130-116-486) and Ki67 (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD4 T cells were enriched with autoMACS Pro Separator (Miltenyi Biotec) using CD4 (L3T4 ...
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bioRxiv - Cancer Biology 2023Quote: ... protocol 37_m_TDK_2 of the gentleMACS Dissociator (Miltenyi Biotec, 130-095-937) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... using CD4 (L3T4) microbeads (order no. 130-117-043, Miltenyi Biotec).
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bioRxiv - Cancer Biology 2023Quote: Tumors were harvested and manually dissociated into small pieces in gentleMACSTM C tubes (Miltenyi Biotec) with 5ml RPMI 1640 medium with 100U/ml Collagenase I (Thermofisher 17100017 ...
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bioRxiv - Cell Biology 2023Quote: Cells expanded following patient biopsy collection were subsequently enriched for myoblasts using anti-CD56-coated microbeads (Miltenyi Biotec GmbH). Primary vials were sourced ...
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bioRxiv - Immunology 2023Quote: ... Untouched T cells were isolated from PBMCs by negative Magnetic-activated cell sorting (MACS) using the human pan T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The hippocampus was extracted and dissociated using the Neural Tissue Dissociation Kit (Miltenyi Biotec), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Brain tissue was dissociated to a single cell suspension using the neural tissue dissociation kit (Miltenyi Biotec), and the resulting cell suspension was centrifuged at 400g for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... 14 by mixing cut tumors with Mouse Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Genetics 2023Quote: ... HSCs were harvest from the culture supernatant and were purified by MACS using CD34 MicroBeads (Miltenyi Biotec, 130-046-702).
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bioRxiv - Genetics 2023Quote: ... labeled with CD144 (VE-Cadherin) MicroBeads (Miltenyi Biotec 130-097-857), and separated by OctoMACS™ Separator.
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bioRxiv - Genetics 2023Quote: PBS-perfused brains were cut into small pieces and dissociated using the Adult Brain Dissociation Kit and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The single-cell suspension was used for the isolation of neurons using the Adult Neuronal Isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... cell debris was removed using MACS Debris Removal Solution (130-109-398, Miltenyi Biotec). Cells were then filtered through a 40 μm cell strainer (40ICS ...
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bioRxiv - Immunology 2023Quote: Circulating CD8+ T lymphocytes were isolated from fresh heparin blood by negative selection using magnetic beads (Miltenyi Biotec) from PBMC ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Liver tissue was dissociated by following the ‘preparation of single cell suspensions from mouse liver’ protocol from Miltenyi Biotec. Cells were then washed in cold PBS+ 2% FBS buffer before staining ...
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bioRxiv - Immunology 2023Quote: ... The C tubes were then placed on a gentleMACS dissociator (Miltenyi Biotec) and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Specificity controls (human FcR blocking reagent (Miltenyi Biotec), FMO ...
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bioRxiv - Immunology 2023Quote: ... Microglia used for qRT-PCR analysis were further sorted using CD11b MicroBeads (Miltenyi Biotec) and immediately lysed for RNA extraction ...
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bioRxiv - Microbiology 2023Quote: An asynchronous parasite culture was washed in RPMI medium and then passed through a MACS LD magnet column (Miltenyi Biotec). NF54attB parasites complete a replication cycle in 44 to 48h under our culture condition and older parasites (>28h old ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Immunology 2023Quote: ... CD25 MicroBeads II and LD columns (Miltenyi Biotec, Cat# 130-092-985 and Cat# 130042901 ...
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bioRxiv - Immunology 2023Quote: ... in RPMI-1640 and running on a gentleMACs (Miltenyi Biotec) for 10–20 min ...
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bioRxiv - Microbiology 2023Quote: ... Nucleoprotein or Hemagglutinin overlapping peptide pools (Miltenyi Biotec: PepTivator® SARS-CoV-2 Prot_S complete ...
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bioRxiv - Immunology 2023Quote: ... and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec) for tumors and lungs respectively ...
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bioRxiv - Immunology 2023Quote: ... cut into small pieces using scissors and placed in a gentleMACS C tube (Miltenyi Biotec) in 5 ml RPMI1640 containing 100 μl 10X collagenase/hyaluronidase enzyme mixture (cat ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were isolated from PBMC using CD14 Microbeads (Miltenyi Biotec) and differentiated into inflammatory macrophages using TPP conditions17 ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were positively selected using CD14 Microbeads (Miltenyi Biotec). Macrophage differentiation was performed using conditions that model chronic inflammation (TPP)17 ...
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bioRxiv - Immunology 2023Quote: ... Lungs were then dissociated for 20 minutes using a custom GentleMACS protocols before filtering through a 70 µm Macs SmartStrainer (Miltenyi Biotec, 130-110-916) into Advanced DMEM/F-12 supplemented with 2% FBS (DMEM-FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Lung tissue was transferred to gentleMACS C-tubes (Miltenyi Biotec) containing 5 ml of freshly prepared lung digestion medium ...
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bioRxiv - Neuroscience 2023Quote: ... treated with blocking buffer containing BSA (Miltenyi Biotec, Cat# 130-091-376) and the ribonuclease inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... endothelial cells were purified using a CD31 MicroBead Kit (Miltenyi Biotec, 130-091-935) and further cultured in the Promocell endothelial cell medium (MV2 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then placed in C Tubes (Miltenyi Biotec, 130-093-237), along with dissociation reagents from the MACS Neural Tissue Dissociation Kit (P ...
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bioRxiv - Genomics 2023Quote: ... The tissues were digested with Human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... and the acquisition of the different samples was performed on MACSQuant (Miltenyi Biotec) flow-cytometer and analysis was completed using FlowJo Software.
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bioRxiv - Cell Biology 2023Quote: ... incubated with CD45+ magnetic microbeads (Miltenyi Biotec, Cat# 130-052-301) and passed through MS Columns (Miltenyi Biotec ...