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Citations for Miltenyi Biotec :
351 - 400 of 838 citations for Human Neuropilin 2 NRP 2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 ng/ml human prolactin (Miltenyi Biotec).
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bioRxiv - Genomics 2020Quote: ... and human anti-CD28 (clone 15E8, Miltenyi Biotec) and cultured at 37°C in a 5% CO2 incubator.
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bioRxiv - Immunology 2019Quote: ... enzymatic digestion (Tumor dissociation kit, human; Miltenyi Biotec) was performed on excised granulomas using gentleMACS octo dissociator ...
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bioRxiv - Cell Biology 2019Quote: ... using the Mitochondria Isolation Kit (human; Miltenyi Biotec). For this ...
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bioRxiv - Immunology 2022Quote: ... 5 ng/ml human IL-7 (Miltenyi Biotec), and 5 ng/ml human FLT3L (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... and 5 ng/ml human FLT3L (Miltenyi Biotec) as described previously 50.
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bioRxiv - Immunology 2020Quote: Human TNFα premium grade was from Miltenyi Biotec and Interferon α-2b INTRONA from Merck MSD France.
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bioRxiv - Immunology 2020Quote: ... and purified using human CD34 Microbeads (Miltenyi Biotec). Isolated CD34+ cells were validated using flow cytometry (CD34-BV650 ...
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bioRxiv - Microbiology 2022Quote: ... and T Cell TransAct™ human (Miltenyi Biotec) for 72 h.
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bioRxiv - Genetics 2022Quote: ... or MACSxpress Neutrophil Isolation Kits human (Miltenyi Biotec) with an aim of 2 × 106 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 25 U/mL human IL-7 (Miltenyi Biotec) and 50 U/mL human IL-15 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 25 U/mL human IL-7 (Miltenyi Biotec) and 50 U/mL human IL-15 (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... or the human CD8+ isolation kit (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2023Quote: ... and 100ng/mL Human IL-4 (Miltenyi Biotec) for 5 days only adding media once in between ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50ng/mL Human IL-4 (Miltenyi Biotec). Monocytes were plated into 6-well plates (5×105 cells ...
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bioRxiv - Neuroscience 2023Quote: ... human and mouse (Miltenyi Biotec, 130-049-601) and a MACS separation system (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... anti-human CD32-APC antibody from Miltenyi Biotec. Ca2+ recordings and live imaging experiments were conducted in physiological buffer containing 140 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... Specificity controls (human FcR blocking reagent (Miltenyi Biotec), FMO ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50ng/mL human M-CSF (Miltenyi Biotec), and 50ng/mL Human IL-4 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... human TGFβ-1 (5 ng/ml, Miltenyi Biotec), anti-mouse IL-4 (11B11 ...
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bioRxiv - Cancer Biology 2024Quote: Human: CD11c APC-Vio770 (MJ4-27G12, Miltenyi Biotec), CD14-PerCP (TUK4 ...
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bioRxiv - Bioengineering 2024Quote: ... human kit (Miltenyi Biotec, cat.: 130-050-301). Knock-in and INDEL frequencies were determined by ddPCR and ICE analysis as described above.
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bioRxiv - Cancer Biology 2024Quote: ... The Human Tumor Dissociation Kit (Miltenyi Biotec 130095929) was used per the manufacturer’s protocol to generate a single-cell suspension from the tumor sample ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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bioRxiv - Developmental Biology 2020Quote: ... cell suspension with PBS supplemented with 0.5% BSA and 2 mM EDTA was applied to an MS column (Miltenyi Biotec, 130-042-201). After negative cells flow through the column ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
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bioRxiv - Microbiology 2023Quote: Approximately half of the lung and the left hemisphere of each brain were weighed and homogenized in 1 mL of DMEM supplemented with 2% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Neuroscience 2023Quote: ... this tissue was transitioned to a 2 ml Dounce homogenizer containing 1.5 ml of nuclei extraction buffer (130-128-024, Miltenyi Biotec, Bergisch Gladbach, Germany) along with 0.2 U/ml Ribolock RNase inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated to human monocyte-derived macrophages (hMDMs) by incubation with 100 ng/ml of recombinant human macrophage colony-stimulating factor (rhMCSF, Miltenyi Biotec) for 6 days at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Synthetic Biology 2020Quote: ... PBMC were isolated from a Buffy Coat from healthy volunteer donors using Ficoll gradient centrifugation and human T cells were isolated using the Human Pan T cell isolation kit (Miltenyi Biotec). Human T cells were cultured in supplemented RPMI media as described for cell lines above ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Human IL-11 (Miltenyi Biotec ref# 130-094-623) and human IL-1β (Enzo Life Sciences ref# ALX-522-056 ...
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bioRxiv - Systems Biology 2019Quote: ... Human cells expressing CD45 (Miltenyi Biotec, 130-045-801) or CD235a (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... digested enzymatically (Miltenyi Biotec Whole Skin Dissociation Kit, human) for 2 hours and further dispersed using the Miltenyi gentleMACS Octo Dissociator ...