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Citations for Miltenyi Biotec :
301 - 350 of 838 citations for Human Neuropilin 2 NRP 2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... The muscle was removed from the -80 °C freezer and transferred to -20 °C where it was minced into pieces of 1-2 mm before being transferred to gentleMACS M-tubes (Miltenyi Biotec) tubes kept on ice ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from 48 healthy individuals (samples taken before the SARS-CoV-2 outbreak) were enriched for CD19+ cells with CD19-microbeads (Miltenyi Biotec). For each individual ...
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bioRxiv - Immunology 2022Quote: ... cells were washed with 2% FBS containing PBS and incubated with 1:20 FcR blocking reagent (Cat. #130-059-901, Miltenyi Biotec) in 2% PBS for 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were incubated for 72 hours at 37°C with either anti-CD3/anti-CD28 beads (1:2 bead-to-cell ratio, Miltenyi Biotec) or 200ng/mL N-803 in the presence of 50µM raltegravir and 5µM saquinavir ...
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bioRxiv - Immunology 2022Quote: B cells were purified from the spleens of mice (aged 2–7 months of age) through negative selection with anti-CD43 magnetic beads (Miltenyi Biotec) and subsequently cultured in RPMI 1640 media (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2023Quote: ... Cells (5×106) were infected at 5000 TCID50 / 1×106 cells at 37 °C for 2 hours on a cell-rotator (Miltenyi Biotec). After infection ...
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bioRxiv - Neuroscience 2023Quote: ... for all male cell lines (FD-1, FD-2, Ctrl) and the StemMACS mRNA reprogramming kit (Miltenyi Biotec, Bergisch Gladbach, Germany) for the female Fabry cell line (FD-3;26) ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Immunology 2023Quote: ... T cells were stimulated by the addition of 100 IU/mL IL-2 (Proleukin) and anti-CD3/CD28 stimulation using loaded MACSiBead particles (Miltenyi Biotec) in a proportion of 1:1 of beads to cell ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... rested overnight at a density of 1×107 cells/mL in medium supplemented with 100 IU/mL IL-2 (Miltenyi Biotec). On day 7 ...
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bioRxiv - Bioengineering 2024Quote: ... and rested overnight in a density of 1×107 cells/mL in medium supplemented with 100 IU/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Cell Biology 2023Quote: ... human CD45+ cells were purified from the remaining fresh murine BM cells using human CD45 MicroBeads (Miltenyi Biotec), and subsequently transplanted into twelve- to thirteen-week-old low-dose busulfan conditioned NBSGW female mice ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor samples and sections were enzymatically and mechanically digested using the human Tumor Dissociation Kit (Miltenyi Biotec) to obtain single-cell suspension ...
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec, Bergisch Gladbach, NRW, Germany). Blood CD4+ T cells were purified with a Naïve CD4+ T cell isolation kit II ...
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bioRxiv - Synthetic Biology 2020Quote: ... human (#130-118-342, Miltenyi Biotec, Germany). Samples were subsequently incubated at 25°C for 1 hour with shaking 1000 rpm (Thermo Mixer C ...
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bioRxiv - Synthetic Biology 2020Quote: ... human (#130-100-153, Miltenyi Biotec, Germany) or CD81-PE ...
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bioRxiv - Synthetic Biology 2020Quote: ... human (#130-103-955, Miltenyi Biotec, Germany), CD63-PE ...
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bioRxiv - Cancer Biology 2019Quote: ... and Human Tumor Dissociation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... human Fc blocking reagent (MACS Miltenyi Biotec) was added to this blocking step ...
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bioRxiv - Cancer Biology 2022Quote: ... the human Tumor Dissociation Kit (Miltenyi Biotec) was used ...
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bioRxiv - Bioengineering 2022Quote: ... and human (Miltenyi Biotec # 130-059-901) FcR blocking reagents ...
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bioRxiv - Immunology 2020Quote: ... 50ng/ml human M-CSF (Miltenyi Biotec) was added on day 0 to promote monocyte survival ...
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bioRxiv - Immunology 2021Quote: ... 10ng/mL human M-CSF (Miltenyi Biotec). Adherent cells were detached with 5 mM EDTA in PBS at day 6 ...
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bioRxiv - Bioengineering 2023Quote: ... human (Miltenyi Biotec, Cat. # 130-096-535) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... human (Miltenyi Biotec, Cat. # 130-097-095) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 10 ng/ml human BDNF (Miltenyi Biotec), 3 μM CHIR-99021 (Tocris) ...
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bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with anti-human IgE FITC (IgE21; eBioscience) and anti-IgG1-PE human (IS11-3B2.2.3; Miltenyi Biotec) for 30 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... PE-Vio770 labeled anti-human CD3 and Vioblue labeled anti-human CD4 (all from Miltenyi Biotec, Bergisch Gladbach, Germany) for 10 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
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bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... 2 mM EDTA in PBS) before positive selection of CD14+ monocytes by incubation with magnetic CD14 microbeads (Miltenyi Biotec, 130-050-201) (15 min ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... tissues were cleansed in ice-cold RPMI1640 and dissociated using the Multi-tissue dissociation kit 2 (Catalog No.130-110-203, Miltenyi Biotec, USA) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... + 0.5x GlutaMax (Thermo 35050061) + 1x NEAA (Thermo 11140050) + 1x N-2 (Thermo 17502048) + 1x MACS NeuroBrew-21 (Miltenyi Biotec 130-093-566). On D3 ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Human CD4+ T cells were isolated by positive magnetic selection using human CD4 MicroBeads kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...