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Citations for Miltenyi Biotec :
3851 - 3900 of 3977 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... single cell suspension prepared by physical and enzymatic dissociation followed by MACS sorting by LS columns (Miltenyi Biotec 130-042-401) with CD45+ cell removal using CD45 micobeads (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Immunology 2023Quote: ... Tonsil tissue was roughly cut into smaller fragments using scissors and was further dissociated into a single cell suspension on the gentleMACS (130-096-334, Miltenyi Biotec) program “Multi_C_01_01” two times with a 15 minute incubation in between in a shaking water bath for 15 min-utes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cd11b+ (130-126-725) and Ly6G+ (130-120-337) were isolated from single cell suspensions using UltraPure MicroBeads from Miltenyi Biotec according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
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bioRxiv - Neuroscience 2023Quote: ... positive fraction cells are then run over a second magnetic column for higher purity) was used to elute highly pure CD11b+ cells in 500□μl of autoMACS Running buffer (#130-091-221, Miltenyi Biotec). Following separation ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... cells were stained with APC-labeled mMR1-tet and mMR1-tet positive cells were isolated with anti-APC microbeads and LS columns (Miltenyi Biotec). The isolated MAIT cells were intraperitoneally (IP ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 10,000 cells from each well was seeded into three or four Matrigel-coated 12-well culture plates and fibroblast growth media was replaced with StemMACS iPS-Brew XF stem cell culture media (Miltenyi Biotec) and changed every day ...
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bioRxiv - Immunology 2023Quote: A cluster of differentiation 19 (CD19)-positive B lymphocytes (total B cells) were purified from buffy coats derived from healthy donors using magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were stained with 5 μg/ml of propidium iodide in PBS and analyzed using a MACSquant analyzer (Miltenyi Biotec). Results were analyzed using Flowjo (https://www.flowjo.com).
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bioRxiv - Cancer Biology 2023Quote: ... Dextran-loaded melanoma cells were cultured with LECs for 48 h after which the cells were sorted using MidiMacs separator and LS columns (Miltenyi Biotec). Due to the cessation of the production of these magnetic nanoparticles ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution; Miltenyi Biotec), TGF-β1 (5 ng/ml ...
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bioRxiv - Immunology 2023Quote: Single cell preparations of tumor-infiltrating lymphocytes were made by mechanically disrupting and enzymatically digesting tumors using gentleMACS™ (Miltenyi Biotec). The digestion media contained collagenase IV and dispase II (1 mg/ml each ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Pathology 2023Quote: ... The tissue was thereafter gently triturated with a needle and syringe and subsequently strained through a 70 µm cell strainer (130-098-458, Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... Human blood samples were enriched for T cells using negative bead selection (StemCell) and stimulated in vitro in media containing a 1:100 titer of TransAct (Miltenyi Biotec), 5% FBS and 100 IU/mL of rhIL-2 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with FACS Intracellular Staining Perm Wash buffer and the following antibodies were added to the cells: anti-STAT1 pTyr701 (Miltenyi Biotec), and anti-STAT3 pTyr705 (eBioscience™) ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells ...
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bioRxiv - Genomics 2023Quote: ... Dissociated cells of each slice were collected with 6ml cold D-PBS and apply to a MACS SmartStrainer (70 μm, Miltenyi Biotec) placed on one well of a deepwell 24-well plate following centrifugation at 300×g for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes were isolated from peripheral blood mononuclear cells (PBMCs) with CD14 microbeads and MACS LS column system (Miltenyi Biotec). PBMCs were obtained from the buffy coats of twelve healthy blood donors (eight males and four females ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed once with FACS buffer and then loaded onto a pre-wet LS column (Miltenyi Biotec, #130-042-401) on the MACS Multistand (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: iPS11 wild type (WT) and transgenic cell lines were grown in feeder-free conditions such as hiPSC Brew XF medium (Miltenyi Biotec) in Geltrex (Life technologies ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... iPSCs expressing the pluripotency marker TRA-1-60 were sorted and enriched using magnetic-activated cell separation (MACS) technology (Miltenyi Biotec). Cells were cultured in neural induction medium (neurobasal medium supplemented with 1x neural induction supplement (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI supplemented with 10%FBS and filtered through 30 µm MACS cell strainer (MACS Filters Miltenyi Biotec). The cells were counted to determine concentration and viability using a hemocytometer after which they were subsequently fixed for chromium fixed RNA profiling.
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bioRxiv - Physiology 2024Quote: ... The cells were then washed twice in stain buffer and applied to a magnetic column (cat. no.: 130-042-201, Miltenyi Biotec). The column was washed x 3 with stain buffer ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatants passed through a 40-μm cell filter and subsequently incubated with 100 µl anti-GFP magnetic beads (Miltenyi Biotec) at 4°C for one h ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401, Miltenyi Biotec). For CD206-positive (CD206+ ...
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bioRxiv - Physiology 2021Quote: ... Flow cytometry analysis for assessment of dead cells was performed with the fixable viability dye Viobility 405/452 Fixable Dye (Miltenyi Biotec®). Viobility dyes react with amine groups of proteins and are non-toxic ...
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bioRxiv - Neuroscience 2021Quote: ... 3-week old heterogeneous neuronal cultures were enriched by a negative selection strategy using antibodies against surface markers CD44 and CD184 (recognizing NPCs, glial progenitors and astrocytes)35 using magnetic-activated cell sorting (MACS® - Miltenyi Biotec). After enrichment ...
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bioRxiv - Neuroscience 2020Quote: ... dissociated (see above) and the single cell suspension was used to enrich for OPCs by CD140a microbeads (Miltenyi Biotec, 130-101-502) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... stained samples were diluted 10-fold in 1x PBS and cell concentrations as well as green fluorescence intensities were recorded on a MACSQuant Analyzer 10 (Miltenyi Biotec, Germany).
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bioRxiv - Cell Biology 2019Quote: ... resuspended and incubated with propidium iodide to exclude dead cells prior to analysis with the Macsquant analyzer (Miltenyi biotec, Bergisch Gladbach, Germany). For kinetics analysis of transferrin recycling ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were purified by positive selection and monocytes were isolated by negative selection according to the manufacturer’s instructions (Miltenyi Biotec, Woking, UK).
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bioRxiv - Cell Biology 2019Quote: ... The suspension was incubated for 15 min with dead cell removal paramagnetic beads (130-090-101, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then passed through LS column (130-042-401 ...
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bioRxiv - Genomics 2021Quote: ... CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS) using anti-human CD14 antibody attached to microbeads (Miltenyi Biotec #130050201) on an LS column (Miltenyi Biotec #130-042-401 ...
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bioRxiv - Immunology 2022Quote: ... Gently absorbed mononuclear cell layer was resuspended in PBS to centrifuge at 500g for 5min and the resuspended cell pellets were incubated with CD3 Microbeeds (130-050-101, Miltenyi Biotec, Germany) at 4-8°C for 15min ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Immunology 2022Quote: ... for preparation of single cells as per manufacturer’s instruction using a gentleMACS™ Octo Dissociator with heaters (Miltenyi Biotec; 130-096-427). Cells were passed through a 70 μm filter and washed twice using ice-cold PBS followed by RBC lysis using ACK lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: BAL samples from silica beads and silica treated mice were treated for 10 min with red blood cell lysis solution (Miltenyi Biotec, 170080033) and washed with PBS by centrifugation at 300 g for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected for TSDR demethylation analysis and stored at −80 as cell pellets or expanded with 500-1000 U/mL human rIL2 (Miltenyi Biotec/Chiron) +/− 100 nM rapamycin (Miltenyi Biotec ...
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bioRxiv - Genomics 2024Quote: ... The resulting cells were enriched for microglia with magnetic labeling and positive selection using CD11b MicroBeads (Miltenyi Biotec cat. #130-093-634) and LS columns ...
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bioRxiv - Genomics 2024Quote: ... Myelin were removed from the single-cell suspensions using negative selection with Myelin Removal Beads II (Miltenyi Biotec cat. #130-096-733) and LS columns (Miltenyi Biotec cat ...
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bioRxiv - Physiology 2023Quote: ... The suspension was incubated for 15 min with dead cell removal paramagnetic beads (130-090-101, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then passed through LS column (130-042-401 ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells from mice immunized with SRBC for 10 days were pre-enriched by B220 MicroBeads (Miltenyi Biotec; 130-049-501) followed by flow sorting ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in 400 μl of buffer with 30 μl of CD31 MicroBeads (130-097-418; Miltenyi Biotec, Bergisch Gladbach, Germany), 30 μl of CD45 MicroBeads (130-052-301 ...
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bioRxiv - Bioengineering 2023Quote: ... tumor spheroids cultured in a 96-well low-adhesion plate or within a hole within a microfluidic device were dissociated into single cells using TrypLE and receive FcR Block (Miltenyi Biotec, Germany), were stained with BV421-CD45 ...