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Citations for Miltenyi Biotec :
3751 - 3800 of 3977 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... tumour suspensions were mashed through a 70 μm filter and live cells were enriched using debris removal solution (Miltenyi Biotec). For obtaining single cell suspensions for the KM macrophage datasets ...
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bioRxiv - Cell Biology 2022Quote: The fluorescence intensities of the recovered cells were analyzed using a MacsQuant Analyzer flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and epithelial cells were selected using CD326 (EpCAM) magnetic beads (respectively 130-052-301 and 130-105-958, Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ expressing thymocytes were depleted using anti-CD4 and CD8 magnetic-activated cell-sorting (MACS) beads (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... bacterial cells were washed again and re-suspended in PBS before they were analyzed with a MACSQuant cytometer (Miltenyi Biotec). C3 deposition analysis in age-matched WT ...
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bioRxiv - Bioengineering 2023Quote: ... the yeast cells were washed once with PBSB and incubated with 750 μL of Protein A microbeads (Miltenyi Biotec, 130071001) per 109 cells ...
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bioRxiv - Genomics 2024Quote: ... we processed a fraction of the dissociated tumor for immune cell depletion using CD11b MicroBeads (Miltenyi Biotec #130-097-142) following manufacturer’s protocol on LS Columns ...
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bioRxiv - Immunology 2024Quote: Macrophages were isolated from lung single-cell suspensions using microbeads conjugated to anti-F4/80 antibodies according to the manufacturer’s protocol (Miltenyi Biotec). CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... These cells were then subjected to staining with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed twice with FACS buffer and analyzed using flow cytometry (MacsQuant Analyzer 10 flow cytometer, Miltenyi Biotec). The FlowJo™ software (BD Biosciences ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... lung single-cell suspensions were labeled with anti-CD4+ or anti-CD8+ magnetic beads and separated by autoMACS (Miltenyi Biotec), or CD4+CD8+ double positive cells purified from thymus single-cell suspensions by flow-cytometric sorting on FACS Aria (BD Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated with conjugated antibodies (FITC-CD11b, BD Biosciences, #557396; PE-Recombinant-ACSA2, Miltenyi Biotec, #130-116-244) for 45 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Murine neutrophils were isolated from bone marrow of 7–12-week-old mice using an Anti-Ly-6G MicroBeads kit (Miltenyi Biotec; Cat. No. 130-120-337) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The telencephalon of the 10-month-old fish were dissected in ice-cold PBS and directly dissociated with Neural Tissue Dissociation Kit (Miltenyi Biotec, Cat. No. 130-092-628) as described previously24 ...
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bioRxiv - Cancer Biology 2021Quote: ... which were negatively selected using a magnetic cell sorting system (MACS) consisting of CD11b and CD45 MicroBeads and MS Columns (Miltenyi Biotec), were used as BM-MSCs ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM EDTA in PBS) and endothelial cells (CD31+) and leukocytes (CD45+) were sequentially positively selected using CD31 and CD45 MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... Monocytes were isolated from PBMCs using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (130-050-201; Miltenyi Biotec). CD14-positive monocytes were differentiated in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... Stem cell marker expression was validated in isolated cell lines by flow cytometry using anti-SSEA4-FITC (Cat. # 130-098-371, Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Neuroscience 2021Quote: ... with either magnetic-activated cell sorting (MACS) using large cell columns and CD90 pre-conjugated to microbeads (#130-042-202 and #130-049-101, Miltenyi Biotec), fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2019Quote: ... Neutrophils were counted using an Invitrogen automated cell counter and the purity assessed by flow cytometry using FITC anti-CD16 and APC anti-CD15 antibodies (Miltenyi Biotec) as markers characteristic of human neutrophils.
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bioRxiv - Immunology 2019Quote: ... T cells were isolated by magnetic selection of CD4 or CD8 BM cells or splenocytes using the Miltneyi MACS system (QuadroMACS Separator, LS columns, CD4 [L3T4] MicroBeads, CD8a [Ly-2] MicroBeads; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed two times with PBS and the percentage of PI stained cells was determined using a MACSQuant VYB system (Miltenyi Biotec). Data analysis was performed using the FlowJo software.
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bioRxiv - Cancer Biology 2019Quote: ... Fresh BM aspirates from MM patients were routinely depleted from malignant plasma cells using anti-CD138-coated magnetic beads (Cat. #130051301 Miltenyi Biotec) and used for RNA sequencing and functional assays.
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bioRxiv - Cancer Biology 2019Quote: ... whole lungs from mice bearing MDA-LM2 macrometastases (3 weeks post tail vein injection) were harvested and lysed in 1 ml Cell Lysis Buffer 2 (R&D) using the gentleMACS Dissociator (Miltenyi Biotec). As a control ...
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...
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bioRxiv - Bioengineering 2021Quote: ... We suspended microbead pellets in additional sorting buffer (500 μL for 108 cells) and then loaded the suspended microbeads to a LS column (Miltenyi Biotec). We washed the column that was attached to a magnetic MidiMACSTM separator from Miltenyi Biotec for retaining CD3 magnetic microbeads with the sorting buffer three times to remove CD3-negative cells and then transferred the beads bound with T cells to a 15 mL falcon tube ...
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bioRxiv - Physiology 2021Quote: Bone marrow monocytes and neutrophils were isolated from C57BL/6J mice using MACS cell separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation with either anti-Ly6G-PE (clone 1A8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Pulse-treated cells and non-treated cells harboring a different fluorophore were then mixed in a 50:50 ratio confirmed by flow cytometry (MACSQuant; Miltenyi Biotec). Gates were established using non-fluorescent parental cell lines as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and then depleted of lineage-positive cells by passing through a magnetic separation column (MACS LD Column, Miltenyi Biotec) loaded on a DynaMag-5 Magnet (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The dispersed islet cells were then resuspended in 150 ml of Nicoletti buffer containing 50 mg/ml propidium iodide (Miltenyi Biotec), 0.1% (wt/v ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 μL of 1x AnnexinV Binding buffer was added to each tube and cells were analyzed on a MACSQuant VYB (Miltenyi Biotec). A minimum of 10 000 cells were analyzed per condition ...
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bioRxiv - Physiology 2022Quote: ... The cell pellet was re-suspended in a sorting buffer and then passed through a magnetized LS separation column (Miltenyi Biotec). The iron-containing superparamagnetic cells/aggregates were eluted from the demagnetized column ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Scotland) followed by CD14+ selection via magnetic cell separation using MACS CD14 MicroBeads and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), as previously described (31) ...
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bioRxiv - Immunology 2022Quote: ... we assessed the capacity of NK cells to perform ADCC after being stimulated with IFN-α or IL-15 (Miltenyi Biotec). In such cases ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells resuspended with 400 µl of Running Buffer composed of MACS BSA stock solution (Miltenyi Biotec) 1:20 with autoMACS Rinsing Solution (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... Biopsies were manually homogenized using sharp dissection between digest steps and by blunt homogenization through a 100 μm cell strainer (Miltenyi Biotec). Centrifugation steps were performed at 300 x g for 5 min at 4°C.
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bioRxiv - Immunology 2020Quote: ... the corresponding mAbs were added for 30 minute and cells were washed with BD Perm/Wash Buffer 1X before acquisition in the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). The percentage of positive cells for CD107a ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were passed through a 45 μm Nitex mesh to remove undissociated cell clumps and after addition of myelin removal beads (Miltenyi Biotec), passed through a MACS column (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence spillover compensation for cell sorting was performed using lymphocytes stained with CD4 (OKT4) in the respective colors and with compensation beads (Miltenyi Biotec) for flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: ... incubated for 15 min at room temperature and applied onto the dead cell removal column (cat # 130-042-401, Miltenyi Biotec), which was attached to the MACS separator ...
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bioRxiv - Immunology 2022Quote: ... Remaining cells were then magnetically labeled with HLA-G – PE antibody and anti-PE microbeads and enriched for HLA-G+ EVTs by magnetic-activated cell sorting using an OctoMACS separator and MS columns (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2022Quote: ... MDSC enriched bone marrow cells were collected from culture as described above and subjected to M-MDSC magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was passed through an LS column to hold the micro bead-labelled cells (RGCs) in the magnetic field of a MidiMACS system (Miltenyi Biotec). The adherent RGCs were rinsed with 3 ml of DPBS/BSA buffer to deplete endothelial cells and microglia ...