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Citations for Miltenyi Biotec :
301 - 350 of 587 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and IL-2 (50 IU/mL; Miltenyi Biotec), and stimulated with Dynabeads (Gibco ...
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bioRxiv - Immunology 2022Quote: ... IL-2-PE (Clone M7/48A, Miltenyi Biotec), CD14-PerCP (Clone TUK4 ...
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bioRxiv - Cancer Biology 2023Quote: ... containing IL-2 (Miltenyi Biotec, # 130-097-748), human serum AB (Seralab ...
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bioRxiv - Cell Biology 2023Quote: ... and 25 ng/mL IL-2 (Miltenyi Biotec). NCI-H1299 ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Microbiology 2021Quote: ... Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S- or N-peptide pool (2 µg/ml, Miltenyi Biotec, USA) for 24 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... adjusted at 5×105 cells/ml and cultured 2-5 additional days in cell culture medium supplemented with 20 ng/ml IL-2 (Miltenyi Biotec) before being used in the experiments.
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bioRxiv - Microbiology 2021Quote: ... tissue samples were weighed and placed in gentleMACS M tubes (30 mg in 100 μl PBS) and dissociated using a gentleMACS Tissue Dissociator (protein01 program)(Miltenyi Biotec B.V., Leiden, Netherlands). Next ...
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bioRxiv - Microbiology 2021Quote: ... Lungs were removed from animals and the right lobes of the lung were homogenized in 1mL of PBS in Miltentyi C tubes (Miltenyi Biotec 130-096-334) using the m_lung_02 program on a Miltenyi gentleMACS tissue dissociator ...
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bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
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bioRxiv - Bioengineering 2022Quote: ... ≈1 gram of muscle tissues was dissected from the hindlimb muscles of a humanely euthanized mouse and placed into a GentleMACS dissociation tube (Miltenyi Biotec, #130-096-334). 7 mL of DMEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The remaining submucosal tissue was placed in a conical tube containing fresh RPMI-1640 supplemented with digestion enzymes from the human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) and chopped into small pieces using dissection scissors ...
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bioRxiv - Developmental Biology 2024Quote: Dissected hearts were cut into small pieces using fine dissecting scissors and transferred to gentleMACS C-tubes (Miltenyi Biotec, Cat-Nr:130-093-237) containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... cells were re-plated with 10 uM StemMACSTM Y-27632 ROCK inhibitor (ROCKi; Miltenyi Biotec). Media was changed every 24 hours.
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bioRxiv - Genetics 2020Quote: ... depletion of lineage+ cells from MNCs was performed using Biotin labeled Lin+ cocktail and Streptavidin MicroBeads (Macs; Miltenyi Bio-tec) and magnetic columns (Macs; Miltenyi Biotec). For cell sorting of LSK ...
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bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4 °C before being washed in flow cytometry buffer and re-suspended in 100 µl antibody staining cocktail (antibodies and FcR blocking reagent (Miltenyi Biotec) diluted as indicated above) ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were washed with 2% FACs buffer (PBS with 2% FBS) prior to resuspension in TexMACS complete media (130-097-196, Miltenyi Biotec, USA), supplemented with 10% FBS ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... ACSA-2 clone IH3-18A3 in PE (Miltenyi Biotec); CD31 clone 390 in PerCP/Cy5.5 (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-2 (100 IU/ml, Miltenyi Biotec), PHA (0,25 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec, cat #130-090-487) and TNF-α-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... and Prot_S (each at 2 μg/ml; Miltenyi Biotec). Negative control wells lacked peptides ...
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bioRxiv - Molecular Biology 2023Quote: ... 2% NS-21 (MACS NeuroBrew-21 supplement (Miltenyi Biotec)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2% NS-21 (MACS NeuroBrew-21 supplement (Miltenyi Biotec)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2% NS-21 (MACS NeuroBrew-21 supplement (Miltenyi Biotec)) ...
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bioRxiv - Physiology 2023Quote: ... 2% MACS® NeuroBrew-21 (Miltenyi Biotec, Surrey, UK), 2 μM PD0332991 (Tocris) ...
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bioRxiv - Neuroscience 2024Quote: ... the ACSA-2 kit (#130-097-678, Miltenyi Biotec) and CD11b (#130-093-634 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml human FGF-2 (Miltenyi Biotec). Then ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 100 IU/mL IL-2 (Miltenyi Biotec)) ...
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bioRxiv - Neuroscience 2023Quote: ... ACSA-2 clone IH3-18A3 in PE (Miltenyi Biotec); CD31 clone 390 in PerCP/Cy5.5 (BioLegend) ...
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bioRxiv - Bioengineering 2024Quote: ... 100 U/ml human IL-2 IS (Miltenyi Biotec) and 1 ng/ml IL-15 (Miltenyi Biotec ...
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bioRxiv - Physiology 2019Quote: ... A 100 mg of depilated skin sample was homogenized thoroughly in 2.0 ml of acetonitrile in a gentleMACS™ M tube using a gentleMACS™ Dissociator (Miltenyi Biotec Inc., CA, USA). Each sample was subjected to three cycles of the preprogrammed homogenization protocol Protein_01.01 (a 55 s homogenization cycle with varying speeds and directions of rotation) ...
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bioRxiv - Neuroscience 2021Quote: Brain tissue samples were weighed and placed in gentleMACS M tubes (30 mg in 100 µl PBS) and treated using a gentleMACS Tissue Dissociator (protein01 program)(Miltenyi Biotec B.V., Leiden, The Netherlands). Next ...
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bioRxiv - Genomics 2023Quote: ... the tissue was chopped into small pieces with scissors and then transferred along with the lysing buffer into a gentleMACS C tube (Miltenyi Biotec, Cat. 130-096-334). An additional 1 ml of lysing buffer was added into the C tube to make a final volume of 2 ml ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... frozen brain sections were thawed in ice cold Nuclei Extraction Buffer (cat#: 130-128-024, Miltenyi) in gentleMACS™ C-Tubes (cat#: 130-093-237, Miltenyi Biotec, Bergisch Gladbach, Germany), followed by automated gentleMACS™ Octo dissociation (cat# ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... liver and brain samples were homogenised in PBS (1-3 mL, adjusted to tissue weight) using gentleMACS™ M tubes (Miltenyi Biotec Ltd, Bisley, Surrey) and gentleMACS™ octo dissociator using the standard protein program ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells ...
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bioRxiv - Cancer Biology 2022Quote: ... were processed and re-suspended in 80 µl MACS buffer (2% FBS, 2 mM EDTA in PBS) and 20 µl CD117 MicroBeads (MACS Miltenyi Biotec, Surrey, UK) to enrich LSK cells as per the manufacturers protocol ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 3 × 105 cells harvested from the spleen or cervical lymph nodes were incubated for 16 h in the presence of a peptide pool derived from the S protein of SARS-CoV-2 (a mixture of PepTivator SARS-CoV-2 Prot_S, S1, and S+; Miltenyi Biotec, Bergisch Gladbach, Germany). After washing the cells with PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Lungs were homogenized RIPA buffer with protease inhibitor using a tissue dissociator (GentleMacs Dissociator Miltenyi Biotec). Homogenates were then centrifuged at 10,000 rpm and 4°C and the supernatants were collected and prepared for either Lc3 or Atg16L1 detection as described above.
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... cells were cultured in the presence of ROCK Inhibitor (10 µM; 130-106-538, Miltenyi Biotec) for 24 h ...