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Citations for Miltenyi Biotec :
251 - 300 of 587 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Freshly resected tumor or adjacent normal lung tissue was thoroughly chopped with surgical scissors and transferred to GentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... 50mg of tumor tissue was disrupted in 1.5ml TRIzolTM using a gentleMACSTM dissociator and M tubes (Cat-No. 130-093-235 & 130-096-335, Miltenyi Biotec, Germany). RNA integrity was evaluated with Agilent Bioanalyzer 2100 and RNA 6000 Nano Kit (Cat-No ...
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bioRxiv - Microbiology 2020Quote: ... One gram of each tissue was homogenized in 9 ml of PBS in gentleMACS M tubes using the appropriate setting on the gentleMACS dissociator (Miltenyi Biotec). Homogenates were filtered through 40-μm-pore-size filters and an aliquot was used to determine viable counts ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 μL of 1x AnnexinV Binding buffer was added to each tube and cells were analyzed on a MACSQuant VYB (Miltenyi Biotec). A minimum of 10 000 cells were analyzed per condition ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
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bioRxiv - Cancer Biology 2022Quote: ... The piece of cerebellum was dissociated into a single cell suspension by a gentleMACS Octo Dissociator within a gentleMACS C Tube in combination with the Brain Tumor Dissociation Kit (P) (Miltenyi Biotec) following all associated manufacturer’s protocols ...
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bioRxiv - Neuroscience 2022Quote: ... the contents of the gentleMACS™ C Tube were strained through a 70 μm cell strainer (Miltenyi Biotec, # 130-110-916) placed on top of a 15 mL conical centrifuge tube ...
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bioRxiv - Genetics 2022Quote: ... kidneys were quickly dissected after mouse euthanization and transferred to gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in 2ml of digestion buffer (13.5 ml of Dispase (STEMCELL ...
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bioRxiv - Immunology 2022Quote: The tissue pieces were rapidly transferred into the gentleMACS C Tube containing Human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2020Quote: ... The cell suspension is pipetted through Falcon tubes with cell-strainer caps and then analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec) for 10,000 events.
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bioRxiv - Cancer Biology 2021Quote: ... we dissociated tumor cells from a fresh primary HB tumor using an enzyme mix in a gentleMACS C Tube (Miltenyi Biotec). Cells were spun ...
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bioRxiv - Immunology 2021Quote: ... Samples were then transferred to 15 mL conical tubes and non-memory B cells were depleted using the AutoMACS Magnetic Sorter (Miltenyi Biotec) “deplete” program ...
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bioRxiv - Immunology 2021Quote: Mouse spleens and lymph nodes were removed from RNAlater solution and transferred to gentleMACS M tubes (Miltenyi Biotec 130-093-236) and 2 ml microtubes (OMNI 19-629-3 ...
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bioRxiv - Genetics 2022Quote: ... Human tonsil tissue was processed by mincing with scissors followed by transfer of up to 4g of tissue to a gentleMACS C tube (Miltenyi Biotec) containing 8 mL of phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: Dissociation of fresh Fallopian tube epithelium was done using the Milltenyi gentleMACS Octo Dissociator with heaters and the human Tumor Dissociation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumours were collected into cold PBS then manually cut into small pieces and digested in GentleMACS C dissociation tubes using the GentleMACS tissue dissociator (Miltenyi Biotec) with the manufacture’s enzymes (liver dissociation kit ...
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bioRxiv - Neuroscience 2023Quote: The hippocampi from both hemispheres of Cx3cr1-NuTRAP mice were cut into four pieces and added to the pre-cooled gentleMACS C-tube (#130-096-334, Miltenyi Biotec) pre-filled with 2 mL ice-cold Nuclei Extraction Buffer (#130-128-024 ...
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bioRxiv - Immunology 2023Quote: ... perfused murine lungs were placed in a gentle MACS C tube and mechanically homogenized in 5 ml of PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec). Lung cell suspensions were lysed of RBCs ...
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bioRxiv - Immunology 2023Quote: ... Fatty tissue was removed from the tonsil as much as possible with scalpels and placed into a gentleMACS C-tube (130-096-334, Miltenyi Biotec) with 5ml RPMI containing 0.3mg/ml Liberase (000000005401020001 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor tissue was suspended in 35 uL of lysis buffer per mg of tissue and mechanically homogenized using M tubes (Milteny Biotec) with a gentleMACS Octo Dissociator (Miltenyi Biotec) using program “RNA_02” ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 20mg of tissue per sample was homogenized in RLT plus lysis buffer with 1% beta-mercaptoethanol using gentleMACS M-tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec). RNA extraction for both T-47D and PDX samples was performed using a Quick-RNA RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... and both the sample and collection tubes were placed in a cold MACS Chill 5 Rack (130-092-951, Miltenyi Biotec). Both positive and negative fractions were collected using the separator ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were stimulated with SARS-CoV-2 peptide pools of S and N (2 μg/ml) (Miltenyi Biotec) in the presence or absence of CECs ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 µM rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec) for the first 24 h after passaging ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 12 ng/ml FGF-2 (Miltenyi Biotec) and 20 ng/ml Activin A (PeproTec ...
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bioRxiv - Cancer Biology 2023Quote: ... + 2% BSA (Miltenyi Biotec: 130-091-376) + 0.2 U/μL RNase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (Miltenyi Biotec, 130-097-745) was supplemented (100 unit/ml ...
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bioRxiv - Immunology 2019Quote: ... An antibody cocktail designed for negative selection of purified naïve T cells (Naïve Pan T cell purification kit, Miltenyi Biotec) was used together with extra αCD15/CD14 beads ...
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bioRxiv - Microbiology 2021Quote: ... Fatty tissue was removed from the lymphoid tissues before dissecting the tissues into smaller pieces to allow dissociation using gentleMACS™ dissociation (C) tubes (Miltenyi Biotec) in Advanced RPMI medium supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... The dipping objectives for the tube were an LVMI-Fluor 4X/0.3 and an LVMI-Fluor 12X/0.53 (both from Miltenyi Biotec, Bergisch-Gladbach, Germany), or an HCX APO 20X/0.95 IMM (Leica Microsystems ...
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bioRxiv - Microbiology 2022Quote: ... Tonsil and lung tissue samples were dissected to approximately 1 g pieces and placed into gentleMACS C Tubes (Miltenyi Biotec, Auburn, CA). The weight of each sample was recorded and PBS was added to a 10% weight/volume ...
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bioRxiv - Molecular Biology 2024Quote: ... pooled honey bees (n=30) were dissociated with 15 mL of molecular grade water using gentleMACS™ M Tubes (Miltenyi Biotec, USA) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... The large airways were removed and approximately ~500mg of airway free tissue was collected in a gentleMACS C-tube (Miltenyi Biotec, Auburn, CA) and 5mL digest buffer was added and placed on a gentleMACS octo dissociator for programmed runs ...
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bioRxiv - Immunology 2020Quote: ... 1% penicillin/streptomycin/L-glutamine)] prior to homogenisation in gentleMACS™ C-tubes using the gentleMACS™ dissociator (Miltenyi Biotec, Auburn, California, USA). Samples were incubated at 37°C with shaking to maximise digestion ...
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bioRxiv - Cell Biology 2020Quote: ... Single-cell suspensions were obtained by placing small sections of spleen (<5mm3) with cold phosphate buffer into gentleMACS™ C tubes and utilizing a benchtop gentleMACS™ dissociator (Miltenyi Biotec). MNCs were enriched through gradient separation (density gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS™ C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS™ Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... or the PepTivator SARS-CoV-2 Prot_N peptide mix (covering the complete sequence of the SARS-CoV-2 N protein; both Miltenyi Biotec). As controls splenocytes were either left unstimulated or activated polyclonally with 12.5 µg/mL phytohemagglutinin (PHA) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were resuspended in 8 ml cold PBE buffer (PBS with 0.5% BSA and 2 mM EDTA) and 2 ml anti-PE microbeads (Miltenyi Biotec) per 108 cells and incubated for 15 minutes at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse anti-Cy5/Alexa Fluor 647 (cocktail of antibodies at 1:100 each from Sigma-Aldrich [Cat# C1117, RRID:AB_477654] and Miltenyi Biotec [custom-ordered antibody used in their Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads ...
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bioRxiv - Immunology 2023Quote: Splenic CD4+ T cells derived from OT-II TCR-Tg mice were enriched with Biotin-labeled antibody cocktail (B220, CD8a, CD11b, CD11c, CD19, CD25, NK1.1, and TER-119) with Streptavidin Microbeads (Miltenyi Biotec). CD4+ T cells were further incubated with CD62L Microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... NKM-PE (16-2-4) from Miltenyi Biotec, Vγ7-FITC (kindly provided by P ...
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bioRxiv - Immunology 2020Quote: ... and 100 U/ml hIL-2 (Miltenyi Biotec) and cultured in IMDM media (supplemented with 10 % FBS ...
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bioRxiv - Neuroscience 2021Quote: ... ACSA-2-PE (Miltenyi Biotec, 130-102-365), Mouse Fc blocker (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2022Quote: ... and hIL-2 (50 IU/ml, Miltenyi Biotec).
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bioRxiv - Cancer Biology 2022Quote: ... and IL-2 (100 IU/mL; Miltenyi Biotec) in X-VIVO 15 medium (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 (10 ng/ml) (Miltenyi Biotec). PBMCs were stimulated for 3 days with 2 μg/ml PHA and if indicated ...