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Citations for Miltenyi Biotec :
301 - 350 of 886 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Microbiology 2023Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 spike peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Single-cell suspensions were prepared using Multi Tissue Dissociation Kit 2 (Miltenyi Biotec; Catalog # 130-110-203) following the instructions provided with the kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then incubated with 2 mL of diluted RBC lysis buffer (Miltenyi Biotec, Auburn, CA) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... lymphoid organ cells were pulsed with a SARS-CoV-2 peptide pool (Miltenyi Biotec, # 130-127-309) (1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were positively stained using CD8a (Ly-2) microbeads (Miltenyi Biotec, cat. # 130-117-044) and the negative flow-through fraction was collected ...
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bioRxiv - Cancer Biology 2024Quote: ... with a final concentration of 50 IU/mL of IL-2 (Miltenyi Biotec catalog #130-097-743). Cell-free supernatants were collected ...
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bioRxiv - Cell Biology 2024Quote: ... NM + 1 μM RA + 1 μΜ Purmorphamine (Miltenyi Biotec 130-104-465), was added and changed every other day until D17 ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Late-stage Dd2 parasites in RBCs were magnetically purified from 5% sorbitol-synchronized cultures using MACS LD columns (Miltenyi Biotec) and seeded at 0.8-million RBCs/well in a Seahorse miniplate which was precoated with Cell-Tak cell and tissue adhesive (Corning) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed with magnetic cell-sorting (MACS) buffer (PBS supplemented with 0.5% BSA and 5 mM EDTA) and incubated with CD117 (KIT) MicroBeads (Miltenyi Biotec) on ice for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... lungs and spleens were collected in 5 ml MACS Tissue storage solution (Cat: 130-100-008; Miltenyi Biotec, Gaithersburg, MD) followed by storage at 4 °C until processing ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
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bioRxiv - Immunology 2024Quote: ... were co-cultured with Siglec-Fhi or Siglec-Flow neutrophils (0.05 million) in the presence of 5 ng/ml TGFβ (Miltenyi Biotec), 20 ng/ml IL-6 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... The cell fraction was then depleted with human CD45 beads (100ul beads per 2×108 cells; Miltenyi Biotec) using autoMACS ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Cell samples were fluorescently stained with Anti-ACSA-2-PE (clone: IH3-18A3) (130-102-365, Miltenyi Biotec), examined by a BD FACSCanto II flow cytometer ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... lungs were harvested in 2 ml of Trizol and homogenized using a gentleMACS Octo dissociator (Miltenyi Biotec, Inc.). Total RNA was purified with trizol/chloroform ...
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bioRxiv - Neuroscience 2023Quote: ... Enzyme Mix 2 (15 μl of Buffer Y, 7.5 μl of Enzyme A [Miltenyi Biotec, 130-094-802]) was added for another 5-min ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 and 4 cytokines were added as followed: 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 100 ng/ml GM-CSF (#130-093-864 ...
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bioRxiv - Immunology 2023Quote: ... and rested overnight with TexMACS medium supplemented with 100 IU/ml of recombinant human IL-2 (Miltenyi Biotec).
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and homogenized in STKM buffer (250 mM Sucrose, 50 mM Tris-HCl, 25 mM KCl, 5 mM MgCl2) using the GentleMACS (Miltenyi Biotec) program Protein_01 ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in PBS and cultured for 5 days in serum-free DMEM containing 50 ng/ml BDNF (Miltenyi Biotec Cat# 130-096-285 ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... After another incubation of 30 minutes at 37°C with 5% CO2 the samples are analysed by flow cytometry (MACS Quant, Miltenyi Biotec) and FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Immunology 2022Quote: All cell culture was performed in a humidified incubator with 5% CO2 at 37°C using cRPMI (see above for ingredients) supplemented with 150ng/mL αCD40 (clone FGK-45; Miltenyi Biotec) and 25ng/mL recombinant murine IL-4 (Peprotech ...
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bioRxiv - Molecular Biology 2024Quote: ... and both the sample and collection tubes were placed in a cold MACS Chill 5 Rack (130-092-951, Miltenyi Biotec). Both positive and negative fractions were collected using the separator ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... perfused murine lungs were placed in a gentle MACS C tube and mechanically homogenized in 5 ml of PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec). Lung cell suspensions were lysed of RBCs ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were stained with 5 μg/ml of propidium iodide in PBS and analyzed using a MACSquant analyzer (Miltenyi Biotec). Results were analyzed using Flowjo (https://www.flowjo.com).
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bioRxiv - Microbiology 2024Quote: ... Harvested cells were centrifuged at 300×g for 5 min at 4°C and resuspended in red blood cell lysis buffer (#130-094-183, Miltenyi Biotec) for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were cultured for six days at 37°C/5%CO2 in the presence of either M-CSF (50 ng/mL; Miltenyi Biotec) or GM-CSF (5 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were subcultivated by incubating the cells for 5 minutes at room temperature with StemMACS Passaging Solution XF (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... and PepTivator® SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec, 15 mers with 11 amino acid overlap) covering the entire SARS-CoV-2 S protein ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then stimulated for 18 hours at 37 °C with SARS-CoV-2 S PepTivator peptides (Miltenyi Biotec) or media alone as a negative control ...
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bioRxiv - Cancer Biology 2022Quote: ... Spleens were placed in 3 ml of PBS supplemented with 2% FBS in C tubes and dissociated using program m_spleen_01 on a gentleMACS Octo dissociator with heaters (Miltenyi Biotec). Dissociated tissue was passaged through a 70 μm cell strainer and centrifuged at 1500 rpm x 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... 500 IU/ml IL-2 and 10 ng/ml IL-15 (all the above regents were from Miltenyi Biotec). The above transfection mix was added into NK cell culture seeded in six-well plates (3×106/well ...
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bioRxiv - Genomics 2023Quote: ... pre-coated in 1 mM EDTA and deposited into a 5 mL ice-cold of PEB buffer (PBS, pH 7.2, 0.5% BSA, 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... under continuous rotation at 150rpm and 37°C for 2 hours in MACS Gentle Dissociation C-Tubes (Miltenyi Biotec) to form a cell suspension ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were pre-enriched from spleens of CD45.1 or CD45.1/2 OT-II mice by negative selection using the CD4+ T-cell isolation kit (Miltenyi Biotec). Live (SytoxBlue−)CD25− CD44loCD62L+TCRβ+vα2+ naïve OT-II T cells were FACS-isolated ...
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bioRxiv - Neuroscience 2024Quote: ... then astrocytes were isolated using the anti-ACSA-2 magnetic cell sorting kit and protocol (catalog #130097678, Miltenyi Biotec). RNA was purified and concentrated with proteinase K cell digestion ...