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Citations for Miltenyi Biotec :
101 - 150 of 886 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
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bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 12 ng/ml FGF-2 (Miltenyi Biotec) and 20 ng/ml Activin A (PeproTec ...
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bioRxiv - Cancer Biology 2023Quote: ... + 2% BSA (Miltenyi Biotec: 130-091-376) + 0.2 U/μL RNase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (Miltenyi Biotec, 130-097-745) was supplemented (100 unit/ml ...
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bioRxiv - Cell Biology 2024Quote: ... 40 ng/mL FGF-2 (Miltenyi Biotec) was added instead of mechanical loading ...
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bioRxiv - Immunology 2021Quote: ... Conventional T cells were enriched from FVB/N mouse spleen single-cell suspensions using the mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Microbiology 2023Quote: Approximately half of the lung and the left hemisphere of each brain were weighed and homogenized in 1 mL of DMEM supplemented with 2% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were enriched by positive selection for c-Kit expression using anti-CD117 immunomagnetic beads (130-091-224, Miltenyi Biotec Inc. 1:5 dilution). Second ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec, #130-105-639). The Hofbauer/fibroblast fraction was loaded onto an MS column for magnetic separation ...
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bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HA-Bcl-2 -scFvPZ1 domain for the dual-DROP-CAR was labeled with an anti-HA antibody (1:100, Miltenyi Biotec, 130-120-791) and an anti-mouse secondary antibody (1:100 ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... were stimulated with irradiated (100 Gy) EBV-LCL (20:1 feeder cell:NK ratio) and recombinant human IL-2 (500 U/mL; Miltenyi Biotec, Inc., San Diego, CA) and IL-15(10ng/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... The dissociated cells were enriched for epithelial cells by Magnetic-activated cell sorting (MACS) (buffer: 1x PBS, 1% BSA, and 2 mM EDTA) with CD326 (EpCAM) microbeads (Miltenyi Biotec, 130-061-101) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Human monocytes were isolated from peripheral blood mononuclear cells from healthy donors (convention n°7828, Etablissement Français du Sang, Marseille, France) following CD14 selection using MACS magnetic beads (Miltenyi Biotec, Bergisch, Germany) as previously described (19) ...
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bioRxiv - Molecular Biology 2024Quote: ... pooled honey bees (n=30) were dissociated with 15 mL of molecular grade water using gentleMACS™ M Tubes (Miltenyi Biotec, USA) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were resuspended in 8 ml cold PBE buffer (PBS with 0.5% BSA and 2 mM EDTA) and 2 ml anti-PE microbeads (Miltenyi Biotec) per 108 cells and incubated for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and 100 U/ml hIL-2 (Miltenyi Biotec) and cultured in IMDM media (supplemented with 10 % FBS ...
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bioRxiv - Neuroscience 2021Quote: ... ACSA-2-PE (Miltenyi Biotec, 130-102-365), Mouse Fc blocker (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2022Quote: ... and hIL-2 (50 IU/ml, Miltenyi Biotec).
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bioRxiv - Cancer Biology 2022Quote: ... and IL-2 (100 IU/mL; Miltenyi Biotec) in X-VIVO 15 medium (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 (10 ng/ml) (Miltenyi Biotec). PBMCs were stimulated for 3 days with 2 μg/ml PHA and if indicated ...
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bioRxiv - Immunology 2022Quote: ... IL-2-PE (Clone M7/48A, Miltenyi Biotec), CD14-PerCP (Clone TUK4 ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 (50 IU/mL; Miltenyi Biotec), and stimulated with Dynabeads (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... containing IL-2 (Miltenyi Biotec, # 130-097-748), human serum AB (Seralab ...
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bioRxiv - Cell Biology 2023Quote: ... and 25 ng/mL IL-2 (Miltenyi Biotec). NCI-H1299 ...
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bioRxiv - Molecular Biology 2024Quote: ... The recombinant mouse (rm) CSF-2 (Miltenyi Biotec) was used at a final concentration of 10 ng/ml.
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bioRxiv - Molecular Biology 2024Quote: ... + 2 µM forskolin (Miltenyi Biotec, #130-117-341) were added ...
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bioRxiv - Microbiology 2020Quote: ... 5 million units per kg recombinant mouse IFNα (Miltenyi Biotec) were injected intravenously ...
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bioRxiv - Cancer Biology 2024Quote: ... TL-1 cells were cultured in RPMI-1640 with 10% FBS and supplemented with 200 U/mL IL-2 (Miltenyi Biotec cat # 130-097-743) and NKL cells were cultured in RPMI-1640 with 10% FBS and supplemented with 100 U/mL IL-2.
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Neuroscience 2024Quote: ... The flow through was collected and astrocytes were then acutely isolated using anti-ACSA-2+ (astrocyte cell surface antigen 2) Microbead Kit (Miltenyi Biotec). The flow through cell suspension was incubated in 15 µl of FcR blocking buffer for 10 min at 4°C followed by 15 µl of ACSA-2 microbeads for 10 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... Each fraction was washed with BSA rinsing buffer and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-allophycocyanin MACS® MicroBeads (Miltenyi Biotec, #130-090-855, lot #5190809239). Each fraction was loaded onto an MS column (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were washed with 2% FACs buffer (PBS with 2% FBS) prior to resuspension in TexMACS complete media (130-097-196, Miltenyi Biotec, USA), supplemented with 10% FBS ...
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bioRxiv - Genomics 2023Quote: ... 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec, 130-091-222]).
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bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
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bioRxiv - Immunology 2022Quote: We stimulated the spleen and lung cells from immunized and control animals to detect nucleocapsid protein-specific CD8+ T cell responses with an N protein-peptide pool at a concentration of 1.5 μg/mL/peptide (Miltenyi Biotec; 130-126-699, Germany) at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029 ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... ACSA-2 clone IH3-18A3 in PE (Miltenyi Biotec); CD31 clone 390 in PerCP/Cy5.5 (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-2 (100 IU/ml, Miltenyi Biotec), PHA (0,25 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec, cat #130-090-487) and TNF-α-APC (Miltenyi Biotec ...