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Citations for Miltenyi Biotec :
301 - 350 of 3730 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2021Quote: Mouse tumors were dissociated into single-cell suspension using the Mouse tumour dissociation kit (Miltenyi Biotec) and the gentleMACS™ Octo Dissociator following manufacturer’s instructions and as described in (Taranto et al. ...
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bioRxiv - Immunology 2020Quote: ... Ly6G+ cells were isolated by Neutrophil isolation Kit mouse and F4/80+ cells by Anti-F4/80 Microbeads Ultrapure mouse kit followed the manufactory instructions (MACS, Miltenyi Biotec).
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bioRxiv - Cancer Biology 2020Quote: ... Bone marrow cells were lineage depleted using Mouse Lineage Cell Depletion Kit (Miltenyi Biotec). Cells were stained to identify HSPC with the following antibodies ...
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bioRxiv - Microbiology 2022Quote: ... T cells were enriched from spleen homogenate by magnetic-activated cell sorting (MACS) using Miltenyi Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: CD3+T-cells were isolated from healthy C57/B6 mice using a Miltenyi kit (Miltenyi Biotec, Auburn, CA). Round bottom plates were coated with 10μg of functional CD3 antibody (eBiosciences ...
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bioRxiv - Immunology 2020Quote: ... and T cells were depleted using Thy1.1 or Thy1.2 positive selection and a magnetic separation kit (Miltenyi Biotec). Cells were counted ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8 T cells were isolated from the LNs of NY 8.3 NOD mice using two rounds of the naïve 8.3 CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated and expanded with the T Cell Activation/Expansion Kit (Miltenyi Biotec, Cat No. 130-091-441) according to the vendor’s manual ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ T cells were purified using a magnetic bead separation kit (Miltenyi Biotec, Cat #130-104-075). Rag1-/- mice were reconstituted with CD8+ T cells by intravenous route (3 x 106 cells/mouse ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Immunology 2020Quote: CD8+ T cells were isolated by MACS (Miltenyi Biotec) from draining inguinal lymph nodes of MB49 bearing male and female mice 14 days post implantation (Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were negatively selected by MACS (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated by MACs (Miltenyi Biotec) from the spleens of naïve mice ...
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bioRxiv - Immunology 2023Quote: ... T cells were depleted using CD90.2 microbeads (Miltenyi Biotec). Mice were maintained on 0.03% enrofloxacin in drinking water for up to 1 wk before and for 2 wk after irradiation and then were housed in autoclaved cages with sterile water ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells positively sorted by MACS (Miltenyi Biotec) were assessed for viability by AO/PI staining and counted with a Luna-FX7TM Automated cell counter (Logos Biosystems).
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bioRxiv - Immunology 2019Quote: ... cells were activated with T Cell TransAct CD3/CD28 (Miltenyi Biotec, Germany) at 1:200 concentration 24h after electroporation and the next day the supernatant was removed to withdraw beads ...
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bioRxiv - Cancer Biology 2022Quote: ... the Fc receptor was blocked (Miltenyi Biotec) and cells were stained with the following antibodies for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were mixed with a 20μl cocktail from a Mouse Cell Depletion Kit (Miltenyi Biotec). Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2021Quote: ... lineage negative BM cells were first depleted using mouse lineage cell depletion kit (Miltenyi Biotec) then applied to five-laser FACSAria II sorter (BD Biosciences).
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bioRxiv - Immunology 2019Quote: ... Cells were counted and CD8 T cells isolated using a magnetic activated cell sorting negative selection (MACS) kit according to the manufacturer’s instructions (Miltenyi Biotec, #130-104-075). 96 well plates were coated over night at 4°C with 1µg/mL anti CD3e (BD ...
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bioRxiv - Cancer Biology 2022Quote: Naïve mouse CD4+ T cells were isolated from spleens of Atg5+/+ and Atg5flox/flox mice using Naïve CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Cells (5 × 105 cells in one milliliter per well ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the negative fraction of CD8+ T cells selection was collected and incubated with the Tumor Cell Isolation Kit (Miltenyi Biotec, #130-108-339) for 15 min incubation at 4°C ...
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bioRxiv - Immunology 2024Quote: ... from a cell suspension and harvested from the crushed spleens of OT-I mice using a CD8a+ T-Cell Isolation Kit and magnetic-associated cell sorting (MACS) according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Similarly ...
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bioRxiv - Immunology 2023Quote: ... The CD4+ T cells were then enriched by negative selection using a CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: Primary murine naïve CD4 T cells were isolated from the spleens and lymph nodes of SLE1.2.3 or control mice using the CD4+CD62L+ T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). CD62L positive selection was routinely ∼98% in purity as determined by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: CD3+/CD4+ T cells were purified by positive selection using magnetic separation with a CD4+isolation kit (Miltenyi Biotec) using PBMCs from three donors as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: SIY-reactive CD8+ T cells were isolated from the spleen and lymph nodes from TCR-transgenic 2C CD45.1+ or 2C CD45.2+ Rag2-/- mice using the CD8a+ T cell isolation kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... SVCs were labeled using the mouse Lineage Cell Depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... primary ACC from the mouse was dissociated as single cells using the mouse Tumor Dissociation Kit (Miltenyi Biotec). Cells were then serially transplanted into NSG mice for approximately 6 months ...
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bioRxiv - Bioengineering 2021Quote: ... CD3/CD28-mediated activation of T cells was conducted by using 10 μl of T cell TransActTM (130-111-160; Miltenyi Biotec B.V. & Co. KG) according to manufacturer’s instructions (T cell activation medium) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Immunology 2024Quote: ... suspended cells (mostly T cells) were separated and cultured with TexMACS (Miltenyi Biotec) supplemented with IL-7 and IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched using CD8 MicroBeads (Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... CD8+ T cells were cultured in TexMACS media (Miltenyi Biotec) supplemented with 100 U/ml human IL-2 (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Naive CD4+ T cells (CD62L+ CD44-) were purified (Miltenyi Biotec) using an AutoMACS ...
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bioRxiv - Cancer Biology 2023Quote: ... depleted from CD3+ T cells using CD3 MicroBeads (Miltenyi Biotec) and 1×106 cells were injected intra-hepatically in 30 µl of PBS ...
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bioRxiv - Biophysics 2023Quote: ... T cells were cultured in TexMACS GMP Medium (Miltenyi Biotec), which was supplemented with IL7 (155 U/mL) ...
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bioRxiv - Immunology 2024Quote: ... or 10μL/mL T-cell TransAct αCD3/αCD28 (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were enriched via positive selection (Miltenyi Biotec), resuspended in TRIzol™ (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... B cells were isolated from total splenocytes using naïve mouse B-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2022Quote: Naïve CD8+ T cells were isolated from the peripheral blood of two healthy donors using the Naive CD8+ T Cell Isolation kit (Miltenyi Biotec, 130-093-244) according to the manufacturer’s instructions ...