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Citations for Miltenyi Biotec :
251 - 300 of 3730 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...
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bioRxiv - Immunology 2021Quote: ... for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS) T-cell subset Isolation Kits (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD4+ and CD8+ cells according to the manufacturers’ protocols ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells were isolated from peripheral blood mononuclear cells obtained from healthy donors using Human CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated (Miltenyi Biotec), activated with anti-CD3/CD28 beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD8+ T cells were purified from the spleen of OT-I.Ly5.1 mice using CD8a+ T Cell Isolation Kit II (Miltenyi Biotec). OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2019Quote: ... antigen-specific T cells were separated using the CD154 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s suggestions ...
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bioRxiv - Neuroscience 2021Quote: ... Cell dissociation was performed following the Neural Dissociation Kit (T) (Miltenyi Biotec GmbH, 130-093-231). Plating media was Neurobasal Plus Medium with 2 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were enriched from all samples using a CD4 TIL Microbeads kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD4+ and CD8+ T cells were isolated using the MACS CD4+ / CD8+ isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and depleted of mature T cells using a CD3ε depletion kit (Miltenyi Biotec, #130-094-973). 1-2×106 bone marrow cells were then injected retro-orbitally to each host mouse under isoflurane anesthesia ...
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bioRxiv - Genetics 2022Quote: ... Peripheral T- and B-cells were isolated from whole spleen using Pan T Cell (Miltenyi Biotec,130-095-130) or Pan B Cell (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... T cells were purified from PBMC by magnetic-bead-based negative T-cell isolation (Miltenyi Biotec, Bergisch Gladbach, Germany) as described before (Wabnitz et al. ...
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bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were extracted with the CD117 Microbead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were cultured in complete StemPro-34 serum-free medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested at 37°C for 40 min using the Mouse Tumor Dissociation kit (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The uncollected non-myeloid (CD11b-) cells were processed with the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-095-130) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Pan T cells were isolated from peripheral blood leukocytes using the Pan T cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec). Isolated human T cells were cultured in X-VIVO-15 medium supplemented with 2 % human serum.
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bioRxiv - Immunology 2020Quote: ... we modified the Pan T cell negative Isolation magnetic-activated cell sorting (MACSR) kit (Miltenyi Biotec, Cat #130-096-535) by adding anti-CD45RA biotin (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Treg and non-Treg cells were enriched using MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) from PBMCs of healthy donors and plated (1 × 105 cells per well ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Immunology 2022Quote: ... isolated using the mouse Lineage Cell Depletion Kit (Miltenyi Biotec). Lineage negative HPCs were transduced with either GFPNKG2D-S/L or RFP-Ly49A and cultured in a differentiation-conditioned RPMI medium as described (4).
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bioRxiv - Immunology 2022Quote: ... Mouse hepatic parenchymal cells were digested and non-parenchymal cells were isolated by Mouse Liver Dissociation Kit (Miltenyi Biotec). Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse CAFs were separated from human pancreatic UM2 cancer cells using the Mouse Cell Depletion Kit (MACS Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2021Quote: ... naïve CD8+ T cells from 1G4 mice spleen were enriched using MACS beads (Pan T cell isolation kit II and CD8a isolation kit, Miltenyi Biotec) and labeled with 5μM CellTraceTM Violet (CTV ...
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bioRxiv - Immunology 2020Quote: ... and activated with T Cell TransAct (Miltenyi Biotec); 36-48 h after activation ...
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bioRxiv - Microbiology 2022Quote: ... and T Cell TransAct™ human (Miltenyi Biotec) for 72 h.
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bioRxiv - Microbiology 2022Quote: PBMCs isolated from healthy donors (n=6) were used for CD4+T cell isolation kit (Miltenyi Biotec). CD4+T cells were treated with recombinant IL-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Either CD45 magnetic beads or T cell isolation kit (Miltenyi Biotec, 130-095-130 or BioLegend, 480024) was used to isolate cells of interest ...
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bioRxiv - Immunology 2019Quote: ... before being magnetically sorted using the (Pan T cell isolation kit Catalog # 130-095-130, Miltenyi Biotec). Isolated T cells were then stained using CFSE Cell Division Tracker Kit (Biolegend Catalog # 23801) ...
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bioRxiv - Immunology 2021Quote: ... CTL were negatively selected using paramagnetic beads following column selection (CD8+ T cell isolation kit (Miltenyi Biotec)) ...
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bioRxiv - Bioengineering 2020Quote: ... CD3 and CD28 monoclonal antibodies (beads T cell activation/Expansion Kit, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) were co-cultured with WJ-MSCs (A/ WJ hMSCs ...
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bioRxiv - Immunology 2021Quote: ... pooled spleen and/or lymph nodes were prepared utilizing the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and purified by negative selection through an LS column (Miltenyi Biotec) ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... and CD8 T cells from dLNs were purified using a magnetic bead separation kit (Miltenyi Biotec, Germany). 106 CD8 T cells were injected into the lesions of CD45.1 mice ...
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bioRxiv - Molecular Biology 2020Quote: ... Fc receptor block (Miltenyi Biotec Inc) was added and incubated on ice-water bath for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Fc receptor blocker (Miltenyi Biotec Inc) was added and ice-water bath was used for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... containing Fc receptor blocker (Miltenyi Biotec) and incubated for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... autologous T cells were isolated from patient NSCLC lesions or from healthy donor PBMCs using the Pan T-cell Isolation Kit (Miltenyi Biotec). Autologous T cells were co-cultured with either ...
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bioRxiv - Microbiology 2020Quote: ... through the New York Blood Center and CD4+ cells isolated by negative selection with a Miltenyi CD4 T cell isolation kit II (Miltenyi Biotec). Cell-free virus was produced by transfection of 293T cells in 10 cm plates using polyjet (Signagen) ...
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bioRxiv - Immunology 2020Quote: T cells were isolated from splenocytes of 8-12-week-old mice using a Pan T Cell Isolation Kit II (Miltenyi Biotec) with >90% purity ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from PBMCs by a magnetic bead “negative” isolation procedure using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... such T cells were selected using a human IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions as previously described.38–42 Post-4 PBMC sample cryopreserved after monocyte depletion was thawed and cultured overnight in Yssel’s media (Gemini Bio Products ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using magnet activated cell sorting (MACS) using the Pan T cell Isolation Kit (negative selection) according to the manufacturer’s instructions (Miltenyi Biotec, Germany). Following separation ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions obtained from spleens were processed with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec; Cat#: 130-104-075) and transferred via the tail vein into recipient mice.
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Autologous CD8 T cells were obtained from frozen PBMCs from the same donor using the CD8+ Microbeads human T cell isolation Kit (Miltenyi Biotec). To monitor cell proliferation ...
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bioRxiv - Immunology 2024Quote: ... was used to purify NK cells and the Human Naïve Pan T Cell Isolation kit (Miltenyi Biotec; CAT#130-097-095) was used to purify T cells ...