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Citations for Miltenyi Biotec :
2801 - 2850 of 2886 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and mechanically and chemically digested into single-cell suspensions using the gentleMACS Dissociator and the mouse tumor dissociation kit by Miltenyi Biotec (130-096-730) respectively ...
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bioRxiv - Cell Biology 2022Quote: One million cells suspended in 90 μl of staining buffer (eBiosciences, 00-4222-26) were incubated with 10 μl FcBlock (Miltenyi Biotec, 130-092-575) to reduce nonspecific antibody binding ...
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bioRxiv - Cancer Biology 2022Quote: Thyroid lobes (8 lobes/condition/experiment) were cut in 1mm3 pieces and processed into single cells suspensions with the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) diluted in Ca2+ free DMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with anti-Biotin mAb microbeads to deplete αβT-cells by magnetic separation using AUTOMACS under depleteS protocol (Miltenyi Biotec, Biergisch Gladbach, Germany). DOT cells were generated from αβ-depleted PBMCs cultured in either 96-well plates or G-REX platform (Wilson Wolf Manufacturing) ...
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bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent intensity of 10 000 cells was detected by flow cytometry on a FACS instrument (MACSQuant®VYB, Miltenyi Biotec, San Diego, CA, USA). Data were analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Cell Biology 2023Quote: MuSCs were isolated from the hindlimb muscles of 11–13-week-old mice following MACS using a Satellite Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and anti-integrin α7 microbeads (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells isolated from the bone marrow of donor mice (8–12 weeks old) via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA, USA). Lin- cells were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2024Quote: ... O4+ cells of the same sex were also mixed and kept in culture medium composed by MACS Neuro Medium (Miltenyi Biotec, 130-093-570), 2% MACS NeuroBrew-21 (1x ...
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bioRxiv - Developmental Biology 2023Quote: ... dissociation was performed for 55 mins (mature sample) or 45 mins (blastema samples) and the cells were filtered through a 70 μm MACS SmartStrainer (Miltenyi Biotec 130-098-462). Cells were analysed by FACS (FACSAria III Cell Sorter ...
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bioRxiv - Synthetic Biology 2023Quote: Functional viral titres of retroviral supernatant were calculated using frozen supernatant on primary human T cells activated with TransAct (Miltenyi Biotec; 130-111-160), 10 ng/ml IL-7 (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2023Quote: For primary cardiac myofibroblast enriched cultures cells were first isolated from adult mice hearts using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) and gentleMACS homogenizer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... retinal organoids between 45-55 days of differentiation were enzymatically dissociated with AccuMax and purified by Magnetic Activated Cell Sorting (MACS) with CD90.2 (Thy1.2) MicroBeads (Miltenyi Biotec, cat. no. 130-121-278) as previously described (Sluch et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The cerebral cortex was cleaned of meninges and the entire cell populations were isolated by enzymatic digestion and mechanical dissociation using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, Bergisch Gladbach, GER) according to manufacturer instructions followed by a 30% density gradient centrifugation ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic cell dissociation and debris and red blood cell removal were performed using the Adult Brain Dissociation Kit (130-107-677; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T lymphocytes were purified from total PBMC by Naïve CD8+ T Cell Isolation Kit and MACS® separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Conidia suspensions were prepared by rinsing the plates with sterile distilled water and passed through a 20-µm cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany) to eliminate residual mycelium ...
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bioRxiv - Immunology 2023Quote: To assess immune cells and endothelia from the lung left lobe of lung was digested using a GentleMACS lung digestion kit (Miltenyi Biotec #130-095-927). In brief ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the PMN suspension was analyzed by flow cytometry (CD3-PerCP-/CD66b-FITC+/CD14-PElow cells > 90%, antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... and later monocytes were enriched from the total bone marrow cells using the Monocyte Isolation Kit (BM) from Miltenyi Biotec (Cat: 130-100-629) through a negative selection process following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... dead cells were removed from the culture via a Miltenyi Biotec Dead Cell Removal Kit and MiniMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). The resulting sample of live CD61+ Mks was resuspended in IMDM media supplemented with BIT 9500 ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Neuroscience 2024Quote: ... Myelin Removal Beads were employed for the specific removal of myelin debris from single-cell suspensions (cat # 130-096-433, Miltenyi Biotec, Gaithersburg, MD, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... immune cells from the colonic lamina propria were extracted using the Lamina Propria Dissociation Kit for mice and gentleMACS dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... cells were rinsed 1X with PBS and 1X with autoMACS running buffer (MACS Separation Buffer (130-091-221, Miltenyi Biotec, San Diego, CA, USA). The cardiac Troponin T (TNNT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Cancer Biology 2023Quote: We isolated human umbilical cord blood (hUCB) CD34+ cells from two patients using the Miltenyi CD34 Microbead Kit and MACS separation column (Miltenyi Biotec, Boston, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cell sorting was performed on Control and EIF4G2KD HEC-1A cells following magnetic beads separation of CD133+ and CD133-(CD133 Microbead Kit, Miltenyi Biotec, 130-097-049) and LS-columns (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... isolated mononuclear cells were positively selected for CD34 using the CD34+ Microbead kit Ultrapure (Miltenyi Biotec, San Diego, CA, USA, cat.: 130-100-453). Cells were cultured at 1.5×105–2.5×105 cells/mL in CellGenix® GMP Stem Cell Growth Medium (SCGM ...
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bioRxiv - Physiology 2023Quote: ... males and females were received from the University of Kansas Medical Center Biorepository (HSC #5929) and dissociated into single cell suspension using gentle MACS dissociator (Miltenyi Biotec, Inc., CA, USA) and maintained at 4°C throughout preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... with the following modifications: dissociation was performed for 55 mins and the cells were filtered through a 70 μm MACS SmartStrainer (Miltenyi Biotec 130-098-462). Anterior cells (mCherry-positive plus EGFP-negative ...
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bioRxiv - Bioengineering 2024Quote: ... Canada) for 48 hours or T-cell TransAct (10 uL for each mL of culture, Cat # 130-111-160, Miltenyi Biotec, Bergisch Gladbach, Germany) for 72 hours respectively.
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Immunology 2024Quote: ... and pDCs were purified using a negative selection MACS beads-based assay (Plasmacytoid Dendritic Cell Isolation Kit II human, Miltenyi Biotec, Bergisch Gladbach, GER), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated into a single cell suspension and the debris were removed using the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) and gentleMACS program 37C_ABDK_02 according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Peritoneal washes from ID8-bearing mice were collected on days 41-47 after ID8 tumor cell injection as specified and F4/80+ macrophages were sorted with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) with the typical purity yield >95%.
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bioRxiv - Bioengineering 2024Quote: Freshly enriched EVs from WJ-MSC WT and CD81-Snorkel-tag cell supernatants were immunoprecipitated either with hCD81 exosome isolation kit (Miltenyi biotec; 130-110-914) or µMACS™ HA Isolation Kit (Miltenyi biotec ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected in free RPMI medium and then dissociated for single-cell suspension by the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells counts were determined by flow cytometer Novocyte (Agilent Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were centrifuged and resuspended in a 5% fetal bovine serum in PBS (FBS/PBS) with CD133-PE (Miltenyi Biotec 130-080-801), CD44-FITC (BD Pharmingen 555478 ...
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bioRxiv - Cancer Biology 2021Quote: ... iPS cells were seeded on previously coated Laminin 521 (Biolamina, Sundbyberg, Sweden) plates to support cell viability in StemMACS™ iPS-Brew XF (Miltenyi Biotec, Bergisch Gladbach, Germany) supplemented with 10 µM of Rock inhibitor Y-27632 and 1x CloneR (Stemcell Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... CD146+ pericytes were isolated from digested cell suspensions of Scx+ or ScxGFP mice using anti-CD146 magnetic microbeads (130-092-007, Miltenyi Biotec, San Diego, CA, USA), and placed into 24-well tissue culture dishes (Corning ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... CD45− cells were enriched by autoMACS separation after magnetic labeling of CD45+ cells using anti-CD45-APC followed by anti-APC microbeads (Miltenyi Biotec, Cat. #130-090-855) or anti-CD45 Nanobeads (Biolegend ...