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Citations for Miltenyi Biotec :
2701 - 2750 of 2886 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... tumor spheroids cultured in a 96-well low-adhesion plate or within a hole within a microfluidic device were dissociated into single cells using TrypLE and receive FcR Block (Miltenyi Biotec, Germany), were stained with BV421-CD45 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in 400 μl of buffer with 30 μl of CD31 MicroBeads (130-097-418; Miltenyi Biotec, Bergisch Gladbach, Germany), 30 μl of CD45 MicroBeads (130-052-301 ...
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bioRxiv - Genetics 2024Quote: ... The live cell fraction was pelleted and resuspended in 1X PBS with 0.04% bovine serum albumin (BSA, Miltenyi Biotec # 130-091-376) to a final concentration of 1 × 106 cells/mL (1,000 cells per μL).
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bioRxiv - Cancer Biology 2024Quote: The biopsy and surgical samples used to prepare the single cell suspension were immediately put into a tissue preservation solution (Miltenyi Biotec, Germany) and transported to the field laboratory in an ice bath for immediate preparation of the single cell suspension ...
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bioRxiv - Immunology 2024Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...
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bioRxiv - Molecular Biology 2024Quote: ... The CD34+ cell fraction was then isolated from the MNCs using the MidiMACS system (CD34+ Microbead Kit; Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells from cord blood or adult donors were sorted using microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (Miltenyi Biotec MACS) through positive magnetic bead selection on LS columns.
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bioRxiv - Immunology 2024Quote: ... both CD45.2 DKO and WT polyclonal donor marrow was depleted of mature T cells by magnetic column-based negative selection (Miltenyi Biotec MACS) using an anti-mouse CD5 biotin-conjugated antibody (Clone 53-7.3 ...
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bioRxiv - Cell Biology 2020Quote: Neonatal mouse cardiac myocytes (NMCMs) were isolated using the MACS cell separation kit (Miltenyi Biotec: Catalog #130-100-825 and #130-098-373). Briefly ...
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bioRxiv - Immunology 2021Quote: B cells from spleens and lymph node of naïve mice were purified by magnetic separation using a pan-B cell isolation kit (Miltenyi Biotec #130-095-813), then incubated overnight with 30 μg/ml LPS (Sigma #L2630 ...
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bioRxiv - Immunology 2019Quote: ... Mouse total T and CD8+ native T cells were enriched from spleens and lymph nodes by negative selection using MACS system (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Ly6B.2+ and F4/80+ cells were isolated from splenocytes prepared as described above by immunomagnetic selection using Miltenyi isolation reagents (Miltenyi Biotec, Bergisch Gladbach). BMDMs ...
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bioRxiv - Cancer Biology 2021Quote: ... Freshly resected tumor tissue was dissociated into single cell suspensions using a tumor dissociation kit (130-096-730, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... were isolated from the spleen of naive Rdh10f/f Cd4Cre Rag1-/- OT-I CD45.1+ CD45.2+ (Rdh10CKO) or Rdh10f/f Rag1-/- OT-I CD45.1+ (control) mice using the Pan T Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany). For the adoptive transfer of memory T cells ...
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bioRxiv - Neuroscience 2020Quote: ... the cell suspension was incubated with the OPC MACS cultivation medium (MACS Neuro Medium containing MACS NeuroBrew-21 (Miltenyi Biotec, #130-093-566), GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... containing cell viability dye – APCH7 (cat. no. 65-0865-14, eBioscience, 1:5000) and anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250), a specific marker of astrocytes ...
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bioRxiv - Neuroscience 2021Quote: ... The whole mouse brain was dissociated into a single-cell suspension using brain tissue dissociation kits following the manufacturer’s instructions (Adult Brain Dissociation Kit, Miltenyi Biotec, 130-107-677). Collected cells were then incubated with metal-conjugated antibodies in cell staining buffer (ProductMaxpar Cell Staining Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The resected tumour samples were processed to a single-cell suspension using the commercial Tumour Dissociation Kit (30095929, Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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bioRxiv - Bioengineering 2022Quote: ... MCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Cell Biology 2022Quote: ... were isolated using the Lung Dissociation Kit (130-095-927) and Endothelial Cell isolation protocol using CD31 MicroBeads (130-097-418) according to manufacturer’s protocol (Miltenyi Biotec, Inc. Auburn, CA). After isolation cells were plated onto fibronectin coated dishes and used in assays between day 4 and day 6 post-isolation as detailed below ...
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bioRxiv - Immunology 2022Quote: ... B cells were depleted from OTII splenocytes by magnetic sorting using CD45R (B220) MicroBeads according manufacturer’s instructions (Miltenyi Biotec cat. 130-049-501). For experiments involving cell division analysis ...
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bioRxiv - Immunology 2022Quote: On the day of the experiment HCC and ccRCC samples were thawed according to 10X protocol for primary/fragile cells (protocol “CG000169”, 10x genomics) followed by CD45 positive enrichment according to the manufacturer’s protocol (Miltenyi Biotec, 130-118-780). HNSCC samples were processed freshly without CD45 positive enrichment ...
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bioRxiv - Cancer Biology 2022Quote: ... Mobilized PBMCs or cord blood were enriched for CD34+ cells using magnetic beads according to manufacturer’s protocol (CD34 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany). CD3 depletion and CD34 enrichment were validated by flow cytometry unless specified otherwise ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs from sAML patients were isolated by Ficoll gradient extraction and CD34+ cells were isolated using magnetic enrichment (Miltenyi Biotec #130-100-453) and cultured overnight in SFEMII media (StemCell Technologies #09605 ...
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bioRxiv - Immunology 2020Quote: ... The analysis gating strategy was aiming to detect change in mean fluorescence intensity of cell surface CD303 expression (detected with Miltenyi Biotec clone AC144) on pDC (CD3-18-56-14-CD11C-DR+CD123+CD304+).
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bioRxiv - Neuroscience 2020Quote: ... following the manufacturer’s instruction followed by manual trituration using fire polished Silanized Pasteur pipette and filtration through 70µm cell strainer (Miltenyi Biotec, 130-095-823). Cells were pelleted at 300 × g ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Non-Neuronal Cells Biotin-Antibody Cocktail (Miltenyi Biotec, # 130-115-389) and incubated for 5 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec, Inc. Cat#130-094-363), CD19 and CD107a (BD Biosciences Cat#555800) ...
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bioRxiv - Bioengineering 2022Quote: ... hMCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, Cat. Nr.: 130-098-185). ADCC was measured using LDH release assay (Cytotoxicity Detection Kit (LDH ...
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bioRxiv - Immunology 2020Quote: ... and the CD4+ cells were isolated by positive immunomagnetic selection using MS columns and a mini MACS separator (Miltenyi Biotec, Bergish Gladbach, Germany) according to the instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Memory CD4+ T-cells were isolated from PBMCs of HIV-uninfected individuals by negative selection using magnetic beads (Miltenyi Biotec, Auburn, CA, USA), as we previously described (8 ...
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bioRxiv - Systems Biology 2021Quote: ... Single-cell suspensions were obtained by enzymatically digesting the excised tumors using the Tumor Dissociation Kit and gentleMACS C system (Miltenyi Biotec, Auburn, CA). In addition to following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Depletion of CD45+ cells in ovarian cancer ascites samples and NSCLC was performed using CD45 MicroBeads (Miltenyi Biotec, catalog no. 130-045-801) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... cell suspension with PBS supplemented with 0.5% BSA and 2 mM EDTA was applied to an MS column (Miltenyi Biotec, 130-042-201). After negative cells flow through the column ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
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bioRxiv - Immunology 2020Quote: ... centrifuged and the cell pellet re-dispersed in 100-200 µl of PBS containing 0.5% BSA and the cell fluorescence intensity was measured using a MACSQuant VYB Flow Cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). A minimum of 15 000 events were analyzed for each sample ...
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bioRxiv - Immunology 2020Quote: ... Microglia were purified from total collected cells using CD11b magnetic beads (Mitenyi Biotec #130-049-601) on columns (Miltenyi Biotec #130-042-201). The percentage of microglia among cells was determined by CD45 (Miltenyi Biotec #130-110-632 ...
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bioRxiv - Immunology 2021Quote: ... PDX tumor chunks were minced then enzymatically digested into single cells by using a tumor dissociation Kit (Miltenyi biotec Cat# 130-095-929). Mouse and human cells were separated through magnetic separation ...
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bioRxiv - Immunology 2022Quote: ... Single cells were depleted from both the CD4+ double positive (DP) and CD4+ single positive (SP) cells using a CD4+ (L3T4) positive selection kit (Microbeads; #130-117-043; Miltenyi Biotec, Gaithersburg, USA) flow-through (this contains both double-negative [DN] and CD8+SP cells) ...
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bioRxiv - Immunology 2022Quote: Untouched NK cells were isolated from frozen and thawed PBMCs using magnetic bead isolation according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) and used immediately in co-culture assays ...
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bioRxiv - Microbiology 2022Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector:target ratio of 9:1 ...
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bioRxiv - Systems Biology 2022Quote: ... and T and B cells were depleted by using CD19 and CD3 MicroBeads (Miltenyi Biotec Cat#130-097-055 and #130-097-043) to enrich for myeloid cells ...
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bioRxiv - Molecular Biology 2022Quote: ... CD45- and CD45+ cells were separated using CD45 Microbeads with MACS MS magnetic columns according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany).
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells were magnetically isolated from spleens using CD8a+ negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-104-075). Purified OT-1 T-cells were resuspended in UCM ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ HSPC were enriched from cord blood mononuclear cells (MNC) by magnetic selection using anti-CD34 conjugated magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec, 130-100-453). To obtain a sufficient cell number for ImageStream and Western blot analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec, 130-049-601) and an anti-O4 microbeads antibody were used to isolate microglia and oligodendrocytes ...
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Immunology 2024Quote: ... CD14+ human monocytes were isolated from fresh peripheral blood mononuclear cells (PBMCs) using CD14 MACS MicroBeads (Miltenyi Biotec, Cat. No. 130-050-201) and cultured in MoDC differentiation medium at a density of 106 cells/ml for 6 days ...