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Citations for Miltenyi Biotec :
201 - 250 of 2724 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Dead cells were excluded using a dead cell removal kit (Miltenyi Biotec). The single-cell suspensions were then stained with TotalSeq™-C Mouse antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Live cells were enriched using MACS dead cell removal kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ T cells isolated with Pan T cell isolation kit (Miltenyi Biotec), were stimulated with TransAct Human T cell activation reagent (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... CD56+ cells (Miltenyi BioTec). When appropriate NK cells were fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... CD56+ cells (Miltenyi BioTec). Cells were also stained for NKG2D (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... the negative cells fraction were further selected for the CD8+ positive cells (CD8a+ T Cell Isolation Kit, mouse, 130-104-07, Miltenyi Biotec). For the tetramers binding reaction ...
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bioRxiv - Immunology 2021Quote: ... for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS) T-cell subset Isolation Kits (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD4+ and CD8+ cells according to the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2019Quote: ... Cell suspensions were then filtered through 70-µm cell strainers (Falcon) and depleted of apoptotic and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: NK cells and CD4 T cells were isolated from donor PBMCs using magnetic activated cell sorting and negative selection (Miltenyi Biotec). NK cells were activated for 72h in 300IU/ml rhIL-2 (R&D Systems) ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Autologous or allogenic CD3+ T cells used in T cell co-culture experiments were isolated using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instruction.
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bioRxiv - Immunology 2022Quote: ... Cells were isolated by positive selection of CD4+ T cells using magnetic-activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Cryogenically preserved healthy human peripheral blood T-cells or murine splenic T-cells were magnetically isolated by negative selection using Pan T-cell isolation kits from Miltenyi Biotec. Murine splenocytes underwent RBC lysis (Sigma ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... Enrichment of UCB-derived CD34+ precursors and PB-derived NK cells was accomplished by Magnetic-activated cell sorting using the CD34 MicroBead kit and the NK cell isolation kit (Miltenyi Biotec), respectively.
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bioRxiv - Cancer Biology 2022Quote: ... Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec, Bergisch Gladbach, Germany). Pairs of liver samples from the same experimental group were pooled and subjected to a second round of purification ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Developmental Biology 2020Quote: ... The separation of STO cells from rat spermatogonial stem cells was carried using the Magnetic activated cell sorting (MACS) from Miltenyi Biotec.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human B cells from PBMC were labelled with B Cell lineage-specific CD19 antibodies and removed by magnetic-activated cell sorting by using the Lineage Cell Depletion Kit for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were enriched by incubating cells with CD4 MicroBeads and positively selecting with MACS cell separation MS columns (Miltenyi Biotec). Purified cells were resuspended in RPMI-C with 500 U/mL recombinant IL-2 (eBioscience) ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) or using the EasySepTM Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... B cell enrichment of bone marrow cells was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). MultiScreen-IP Filter Plates (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend) ...
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bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Both CD45 nondepleted and CD45-depleted single-cell suspensions were depleted of dead cells using a Dead Cell Removal kit (catalog no. 130-090-101; Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... IEC/IEL single cell suspensions were prepared as above and dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec) using LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were then purified by negative magnetic cell sorting using mouse CD8α+ T-cell isolation kit (130-104-075; Miltenyi Biotec) according to the manufacture’s protocols ...
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A framework for defining mesenchymal cell types associated with murine periosteal and endosteal bonebioRxiv - Genomics 2023Quote: ... cells were depleted of hematopoietic cells and endothelial cells using a lineage depletion kit (Miltenyi Biotec, cat. no. 130-090-858) followed by CD45 ...
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bioRxiv - Cancer Biology 2023Quote: ... The single cell suspension was then depleted of dead cells and debris using magnetic-activated cell sorting (MACS, Dead Cell Depletion Kit, Miltenyi Biotec). Cells were then distributed drop-wise onto a Seq-Well microwell array preloaded with mRNA capture beads and processed as described previously5 ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells were isolated from peripheral blood mononuclear cells obtained from healthy donors using Human CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manual ...
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bioRxiv - Plant Biology 2020Quote: ... Eluted proteins were analysed by western blot using HRP-conjugated anti-myc antibody (130-092-113; Miltenyi Biotec), or an anti-HA antibody (3F10 ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
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bioRxiv - Immunology 2020Quote: ... The splenocytes were incubated with the spike protein peptide pool at 1.5 μg/ml/peptide (Miltenyi Biotec, Germany) at 37 °C for 16-18 hours ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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bioRxiv - Immunology 2019Quote: ... For genomic and transcriptomic analyses of activated LCs fresh migrated LCs from 3 donors were stimulated with TNF-α (25 ng/ml, Miltenyi Biotec, UK) for 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Dead cells were removed using a MACS Dead Cell Removal Kit (Miltenyi Biotec) and cell concentration determined using a Countess II Automated Cell Counter (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... The dead cell removal was performed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Hematopoietic lineage+ cells were removed with the lineage cell depletion kit (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells were removed using the MACS Dead Cell Removal Kit (Miltenyi Biotec). The resulting single cell suspensions were pooled by experimental group (Kras ON/Kras OFF) ...
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bioRxiv - Cell Biology 2022Quote: ... Obtained cells were enriched for CD11b+ cells by using CD11b MicroBeads (Miltenyi Biotec) and seeded into poly-lysine coated plastic ...
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bioRxiv - Neuroscience 2020Quote: ... cleared of myelin and cell debris (cell debris removal kit, Miltenyi Biotec, Germany), and then filtered through a 70 μm separation filter ...
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bioRxiv - Systems Biology 2022Quote: ... The dead cell removal was performed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and CD25 Microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... and enriched for live cells using a Dead Cell Removal kit (Miltenyi Biotec) before washing with PBS ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched with the B cell isolation Kit II (Miltenyi biotec) and stained with a recombinant ...