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Citations for Miltenyi Biotec :
101 - 150 of 2724 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec) and irradiated donor-mismatched PBMCs (all CAR-T cells ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation for 2.5 h at 4 °C while slowly rotating ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Immunology 2022Quote: ... or S-protein (B.1.351) mutation peptide pool (Miltenyi Biotec, # 130-127-958, Germany) at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were filtered with a 70 μm cell strainer and dead cell removal was performed on single cell suspensions (Miltenyi Biotec) and live tumor cells were frozen in 90% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were isolated by magnetic activated cell sorting (CD14+ Cell Isolation Kit; Miltenyi Biotec). 2×10⁵ monocytes were then differentiated into M1-type macrophages cells by incubation with 1000 U/ml GM-CSF (granulocyte macrophage-colony stimulating factor ...
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bioRxiv - Cancer Biology 2022Quote: ... Red cell lysis and dead cell removal (Miltenyi Biotec) was carried out prior to generation of single cell droplets with 10X Genomics Chromium Controller ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were captured with 100µl of μMACS protein A beads (Miltenyi Biotec; San Diego, CA). Beads were isolated with μ columns and a μMACS separator (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000; Miltenyi Biotec) for one hour at room temperature ...
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bioRxiv - Genomics 2020Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec), and cells were counted again to determine final viable cell numbers.
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bioRxiv - Genomics 2019Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec) and cells were counted again using an automatic cell counter.
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bioRxiv - Cancer Biology 2024Quote: ... the cell suspension was enriched for viable cells using the dead cell removal kit (Miltenyi Biotec) following the manufacturers protocol ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
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bioRxiv - Genetics 2020Quote: ... NK cell populations using cell-type specific kits (Miltenyi Biotec). For NK cells ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) for 1 hr at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2022Quote: RNA immunoprecipitation (RIP) assays were performed with the μMACS GFP-Tagged Protein Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) or HRP-conjugated anti-c-myc antibody (1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then magnetically enriched for total CD4+ T cells (Stemcell EasySep CD4+ T cell Enrichment kit or Miltenyi Biotec CD4+ T cell Isolation Kit). Enriched CD4+ T cells were stained with surface antibodies for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting of CD45+ cells was performed using the MACS cell separation microbeads and reagents (Miltenyi Biotec). Total RNA was extracted with the RNeasy mini kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec) to lyse blood cells ...
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bioRxiv - Immunology 2022Quote: ... Total aortic CD3+ T-cells and CD3- cells were isolated using the Pan T cell kit (Miltenyi Biotec).
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bioRxiv - Microbiology 2021Quote: ... dead cells were removed from the 1 mL cell suspension using a dead cell removal kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Autologous naïve T cells and NK cells were purified by magnetic-activated cell sorting (MACS®; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... single cell suspension was enriched for live cells using Dead Cell Removal kit (130-090-101; Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... The flow-through was pelleted at 1250 rpm in a swinging bucket centrifuge set to 6°C then resuspended in 3 ml cold AutoMACs Running Buffer (AMB, Miltenyi Biotec). After gentle resuspension ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’ s protocol before staining for FACS sorting was performed.
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bioRxiv - Immunology 2022Quote: ... dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec). Live cells were resuspended in Advanced DMEM/F-12 medium containing 17.51mM D-glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were seeded 1000 cells/well in organoid medium in 384-well plates (PrimeSurface® ...
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bioRxiv - Immunology 2022Quote: ... the cells were filtered using a 30µm cell strainer (Miltenyi Biotec), and the Dead Cell Removal Kit was used to remove dead cells to obtain a cell viability of ≥80%.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were first depleted for CD45+ cells (Miltenyi Biotec, SanDiego, CA) and then positively selected for CD31+ cells (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were mixed with 1% alginate (ratio of 1:1 ...
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bioRxiv - Immunology 2022Quote: ... followed by dead cell removal (Dead Cell Removal Kit, Miltenyi Biotec). NK cells were stained with CellTrace Violet as indicated above ...
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bioRxiv - Immunology 2022Quote: ... Plasma B cells were isolated by CD138+ cell enrichment (Miltenyi Biotec CD138+ plasma cell isolation kit ...
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bioRxiv - Cancer Biology 2023Quote: ... Dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...