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Citations for Miltenyi Biotec :
151 - 200 of 269 citations for B Raf Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... B cells were enriched by biotin negative selection using MACS LS Columns (Miltenyi Biotec). The purity of recovered CD19+ B220+ B cells was >97% ...
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bioRxiv - Immunology 2024Quote: Resting B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Primary resting mouse splenic B-cells were isolated using anti-CD43 microbeads (Miltenyi Biotec) and activated with 25 ug/mL LPS (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Monocytes were isolated from PBMCs using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (130-050-201; Miltenyi Biotec). CD14-positive monocytes were differentiated in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... HTLV-1-infected cells were sorted as CD4+CADM1+ T-cells using FITC-labeled anti-CADM1 mAb (3E1, MBL) and anti-FITC MicroBeads (Miltenyi Biotec). The CADM1- fractions were used as normal CD4+ T-cells ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs) for either the 8-color MACSQuant Analyzer (Miltenyi Biotec), or the 14-color LSRFortessa™ X-20 flow-cytometers (BD) ...
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bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
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bioRxiv - Immunology 2021Quote: ... Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec, USA). To obtain high purity Treg ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Immunology 2022Quote: ... Naïve B cells were then purified by negative selection using anti-CD43 microbeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by B cell enrichment using anti-CD19 magnetic beads (Miltenyi Biotec, 130-050-301) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Magnetic columns were used to deplete non-B cells according to standard protocol (Miltenyi Biotec). Cells in effluent were further labeled with NP-PE ...
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bioRxiv - Immunology 2021Quote: ... we purified B cells (Fig. 1D) by positive selection using magnetic beads (MACS, Miltenyi Biotec) conjugated with a monoclonal anti-mouse B220 antibody (clone ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were isolated from 16 healthy individuals and enriched for CD19+ B cells (Miltenyi Biotec). Two times 2.5 × 105 of naive B cells (CD20+IgM+IgD+IgG− ...
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bioRxiv - Immunology 2021Quote: ... B cells were excluded from whole splenocytes by incubating with anti-CD19 beads (Miltenyi Biotec) or by using the EasySep™ mouse CD19 positive selection kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: B cells were isolated from cryopreserved PBMCs by positive selection using CD19+ microbeads (Miltenyi Biotec) and expanded through CD40-CD40L stimulation by culturing cells for 4-5 days with irradiated NIH3T3 feeder cells constitutively expressing CD40L at 37°C in 5% CO2 in B cell medium ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Immunology 2023Quote: ... The cells were subsequently processed using the Pan B Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 50 ng/ml recombinant human granulocyte macrophage colony–stimulating factor (rhGM-CSF) (130-093-865, Miltenyi Biotec), and 50 ng/ml rh interleukin (IL)-4 (130-093-921 ...
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bioRxiv - Immunology 2020Quote: ... in combination with or without recombinant interleukin-4 (IL-4, Miltenyi Biotec, catalogue number: 130-094-061) for 24h ...
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bioRxiv - Immunology 2023Quote: iMSC-like cells were acquired by stimulating ADSCs with recombinant human IL-1β (0.5ng/ml, Miltenyi Biotec) overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: Splenic CD4+ T cells or CD19+ B cells were positively selected using immunomagnetic MicroBeads (Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec). Cells were washed twice with 5 ml FACS buffer with 3 mM EDTA and adjusted to a cell density of 1-2×106 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified with CD43 micro beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany), and 1×106 cells were placed in the top chamber of a transwell plate in 100μL of volume ...
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bioRxiv - Microbiology 2021Quote: ... and B-cells were affinity-purified using CD19 microbeads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) resulting in >95% pure B-cell populations ...
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bioRxiv - Immunology 2022Quote: ... B cells were first isolated from donors’ PBMC by MACS using human CD19 MicroBeads (Miltenyi Biotec). CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes ...
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bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2020Quote: ... was added to B cells and then analyzed by flow cytometry (MacsQuant Analyzer 10; Miltenyi Biotec,). The double labeling procedure allows a distinction into living (Annexin V −/PI−) ...
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bioRxiv - Immunology 2022Quote: ... the PepTivator® SARS-CoV-2 Prot_S B.1.1.529 Mutation Pool (130-129-928, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein B.1.1.529-Omicron (RP30121 ...
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bioRxiv - Immunology 2022Quote: ... PepTivator® SARS-CoV-2 Prot_S B.1.1.529 WT Reference Pool (130-129-927, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein-crud (RP30020 ...
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bioRxiv - Immunology 2022Quote: ... using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Microbiology 2024Quote: CD19+ B cells were purified by positive magnetic separation using CD19 MicroBeads (Miltenyi Biotec, Gaithersburg, MD) and MS or LS columns ...
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bioRxiv - Immunology 2023Quote: ... and rested overnight with TexMACS medium supplemented with 100 IU/ml of recombinant human IL-2 (Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... followed by incubation with anti-Biotin mAb microbeads to deplete αβT-cells by magnetic separation using AUTOMACS under depleteS protocol (Miltenyi Biotec, Biergisch Gladbach, Germany). DOT cells were generated from αβ-depleted PBMCs cultured in either 96-well plates or G-REX platform (Wilson Wolf Manufacturing) ...
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bioRxiv - Microbiology 2023Quote: ... CD14+ cells were isolated from the PBMCs using human CD14+ micro beads conjugated with anti-human CD14 mAbs (130-118-906, Miltenyi Biotec, Bergisch Gladbach, Germany) LC columns (130-042-401 ...
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bioRxiv - Microbiology 2019Quote: ... CD19 microbeads were used for magnetic separation of purified B cells using an autoMACS separator (Miltenyi Biotec). All cell lines in this study were cultured according to standard mammalian tissue culture protocols (ATCC ...
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bioRxiv - Immunology 2021Quote: ... CD19+ B cells were purified from human PBMCs using the REAlease® CD19 Microbead Kit (Miltenyi Biotec) according to the manufacturer’s recommendations with purity greater than 98 %.
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bioRxiv - Immunology 2019Quote: ... untouched B cells were enriched using anti-CD43 magnetic beads (MACS) according to manufacturer’s protocol (Miltenyi Biotec) obtaining > 95 % purity ...
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bioRxiv - Immunology 2020Quote: ... pro-B cells were positively selected from bone marrow using anti-CD19-labeled magnetic beads (Miltenyi Biotec) and were cultured for 24h hr in Opti-MEM supplemented with 15% FBS (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... B lymphocytes were enriched from erythrocyte-lysed spleen cells using CD43 MicroBeads (Miltenyi Biotec,130-049-801) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: B cells were purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec) and subsequently expanded for 10-14 days in RPMI-1640 supplemented with 10% human serum (Bavarian Red Cross) ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were neutralized with Ca2+/Mg2+ free DPBS with 0.5% BSA (Miltenyi Biotec, 130-091-376) and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Human recombinant interleukin-7 (IL-7) and interleukin-15 (IL-15) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Retronectin (Recombinant Human Fibronectin ...
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bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
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bioRxiv - Immunology 2021Quote: ... Naïve mature B cells were isolated from spleen cells by immunomagnetic negative selection with anti-CD43 (Miltenyi Biotec). B cells were cultured in RPMI-1640 medium (Corning ...
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bioRxiv - Cancer Biology 2020Quote: For CSR experiments spleen naive B cells were isolated by immunomagnetic depletion using anti-CD43 beads (Miltenyi Biotec). Purified naïve B cells were cultured at 1.2 x 106 cells/ml in complete RPMI medium supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD19+ B cells were magnetically removed from freshly isolated PBMCs using CD19 Microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s recommendations (depleted condition) ...