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Citations for Miltenyi Biotec :
101 - 150 of 269 citations for B Raf Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Monocytes were isolated using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (Miltenyi Biotec). CD14-positive monocytes were seeded into 12 well plates at 5 × 105 cells/well and differentiated into macrophages by culture in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: PBMCs were activated in plates coated with 1 μg/mL anti-CD3 mAb (clone: OKT3, Miltenyi Biotec) and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from PBMCs using CD19-microbeads (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... Plasma B cells were isolated by CD138+ cell enrichment (Miltenyi Biotec CD138+ plasma cell isolation kit ...
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bioRxiv - Immunology 2022Quote: ... B cells were enriched from PBMCs with CD19 microbeads (Miltenyi Biotec). CD19+ cells were stained with anti-human AF700-CD20 (BD) ...
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745) for 2 days prior to flow cytometry analysis.
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745). HEK293FT cells were a gift from the Salk viral vector core and were cultured in DMEM (high glucose ...
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bioRxiv - Neuroscience 2019Quote: ... human recombinant anti-CD11b/c (1:50) (#130-120-288, Miltenyi Biotec, Germany). Secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... 3% human serum (Seralab) +20 ng/ml human recombinant IL-2 (Miltenyi Biotec) and activated with TransAct reagent (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant human Interferon gamma1b (IFNγ1b) was purchased from Miltenyi Biotec (130-096-481). Exon1-mHTT97Q-eGFP and Exon1-wtHTT25Q-eGFP cloned in pcDNA3.1 plasmids were kindly provided by Dr ...
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bioRxiv - Cell Biology 2024Quote: ... and 4 ng/mL of recombinant basic fibroblast growth factor (Miltenyi Biotec, Germany). Cells were cultured for 2 passages at a seeding density of 5×10 cells/cm.
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bioRxiv - Immunology 2022Quote: ... cells were blocked at 37 C for 15 min with 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec). A stimulation with an equimolar amount of DMSO was performed as negative control ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were blocked at 37°C for 15 min with 0.5 μg/ml anti-CD40 mAb (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2023Quote: ... EGFRt-positive T cells were enriched using biotin-conjugated anti-EGFR mAb and anti-biotin beads (Miltenyi Biotec), and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: B cells were isolated from splenocyte suspensions using CD43 microbeads (Miltenyi Biotec), and plated at a density of 1×106/mL in B-cell media (DMEM high glucose with Glutamax (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... and stained with APC-conjugated anti-Granzyme B (clone REA226, Miltenyi Biotec). Cell viability was assessed by LIVE/DEAD staining according to the manufacturer’s instruction (Molecular Probes ...
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bioRxiv - Genetics 2021Quote: ... and Human CD19+ B Cell Isolation kit (Miltenyi Biotec,130-050-301) respectively.
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bioRxiv - Immunology 2020Quote: ... Stained B cells were investigated with the MACSQuant Analyzer 10 (Miltenyi Biotec) and analyzed by using Flow-Jo or Flowlogic software.
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bioRxiv - Immunology 2021Quote: ... splenocytes were pre-enriched for B cells with B220 microbeads (Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... B cell enrichment kit was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Recombinant mouse CD19 ...
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bioRxiv - Immunology 2021Quote: ... Surface staining was performed in fresh isolated PBMC with a panel of mAbs including: CD45RA-PE (HI100, Miltenyi Biotec), CD4-PercP (VIT4 ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 100 ng/mL of recombinant human flt3 ligand (Miltenyi Biotec, Auburn, CA), stem cell factor (SCF ...
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bioRxiv - Immunology 2021Quote: ... B cells were purified by negative selection (Miltenyi Biotec, Cat#130-091-151) from PBMCs and stimulated with R848 (1 ug/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Naïve B cells were separated by positive selection using AutoMACS system (Miltenyi Biotec) after incubation with anti-IgD-FITC (BD Pharmingen ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... B cells were isolated using CD19 MicroBeads and MACS separation columns (Miltenyi Biotec), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated with 1 μg/ml CpG oligodeoxyribonucleotide 2006 (Miltenyi Biotec), 2.4 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used were: CD19 PE (LT19) and CD138 APC (B-B4; Miltenyi Biotec); CD23 APC (M-L233) ...
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bioRxiv - Immunology 2022Quote: ... with the 37°C Multi-B program of a gentleMACS Dissociator (Miltenyi Biotec). The resulting single cell suspension was passed through a 70 µm cell strainer (Falcon) ...
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bioRxiv - Microbiology 2020Quote: ... we enriched B cells by incubating PBMCs with anti-CD20 MicroBeads (Miltenyi Biotec) followed by magnetic separation on a MACS LS column (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: WT splenic naïve B cells were isolated using CD43 Micro-Beads (Miltenyi Biotec) and stimulated with anti- CD40 (1 µg/ml ...
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bioRxiv - Immunology 2021Quote: WT splenic naïve B cells were isolated using CD43 Micro-Beads (Miltenyi Biotec) and stimulated with 1 µg/ml anti-CD40 antibody (Invitrogen ...
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bioRxiv - Immunology 2020Quote: B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Immunology 2022Quote: Splenic B cells were purified by negative selection using CD43 MicroBeads (Miltenyi Biotec). Splenocytes from WT and Slc29A3−/− mice were labelled with CD43 magnetic beads ...
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bioRxiv - Immunology 2022Quote: ... and CD43-negative splenic B cells were enriched using autoMACS (Miltenyi Biotec, Germany) and subjected to experiments.
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bioRxiv - Immunology 2023Quote: ... B cells were obtained by negative selection using anti-CD43 beads (Miltenyi Biotec), as per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... before cells were incubated in anti-mouse IgG 2a+b microbeads (Miltenyi Biotec) for 20 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Tregs were purified after enrichment of CD25+ cells using biotinylated anti-CD25 mAb (7D4) and anti-biotin microbeads (Miltenyi Biotec), followed by CD4 staining (RM4.5 ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Surface- labelled plasma membrane (PM) microsomes were isolated by magnetic immunoaffinity using anti-FITC mAb-coated magnetic beads (Miltenyi Biotec) and concentrated by ultracentrifugation in thickwall polycarbonate tubes (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted cells were treated with 50 ng/mL recombinant stem cell factor (SCF; Miltenyi Biotec), 5 μM imatinib mesylate (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...
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bioRxiv - Cell Biology 2020Quote: ... Human ESCs were maintained in StemMACS™ iPS-Brew XF (iPS-B, Miltenyi Biotec) on Laminin-521 (L521 ...
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bioRxiv - Cell Biology 2021Quote: ... Splenic B cells were isolated with the CD45R(B220) cell isolation kit (Miltenyi Biotec). The purity of B220 cells was >90% ...
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bioRxiv - Immunology 2019Quote: ... negatively selected B cell fractions with CD23 Biotin and anti-Biotin Microbeads (Miltenyi Biotec)
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bioRxiv - Immunology 2022Quote: ... or S-protein (B.1.351) mutation peptide pool (Miltenyi Biotec, # 130-127-958, Germany) at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were used as starting material to isolate B cells (CD19+ microbeads, Miltenyi Biotec) and monocytes (CD14+ microbeads ...
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bioRxiv - Immunology 2023Quote: B cells were purified from UCBMC using MACS CD20+ microbeads (Miltenyi Biotec, Waltham, MA). 50-100,000 B cells were plated per well and stimulated using a TLR agonist cocktail containing LPS (100 μg/μL) ...