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Citations for Miltenyi Biotec :
1901 - 1950 of 2165 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the resulting pellet was resuspended in 100 μl of microbeads from the dead cell removal kit (Miltenyi Biotec, 130-090-101) and processed according to the commercial protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... CD8+T cells in single-cell suspensions were purified from tumors using a magnetic cell sorting column purification system (CD8+ T Cell Isolation Kit, Miltenyi Biotec). The conditioned medium (CM ...
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bioRxiv - Immunology 2024Quote: ... Human fetal thymus samples were HPCs were obtained from Ficoll-Hypaque–purified CB samples using the CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec), as described (38) ...
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bioRxiv - Immunology 2024Quote: ... The lung was perfused with 1X PBS and processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). Blood was treated with RBC lysis buffer for five minutes ...
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bioRxiv - Neuroscience 2024Quote: ... the tumor was carefully dissected from the affected hemisphere and processed using the Adult Brain Dissociation Kit (130-107-677, Miltenyi Biotec) for digestion ...
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bioRxiv - Neuroscience 2024Quote: Cortices were dissected from P1 and P14 brains and dissociated into single-cell suspension using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628 for P1 and 130-094-802 for P14) ...
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bioRxiv - Immunology 2024Quote: ... and Tconvs (CD4+CD25-) were purified (90-95% pure) by using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: ... was used to lyse red blood cells before T cell isolation using the naive CD4+ T Cell Isolation Kit from Miltenyi Biotec or the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kits from BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The remaining tissue was placed in a gentleMACS C-tube and processed using the gentleMACS Mouse Adipose Tissue Dissociation Kit and the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec). Cell suspensions were diluted to 107 cells/mL and kept on ice ...
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bioRxiv - Immunology 2024Quote: Blood and tonsil myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) were obtained by using CD1c+ Dendritic Cell Isolation Kit (Miltenyi Biotec) and the Diamond Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... and Flt3-Ligand (Bio-Techne) A fresh aliquot of donor cells was thawed and the MACS CD8+ T-cell isolation kit (Miltenyi Biotec) was used according to the manufacturers instruction to enrich for CD8 positive cells ...
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bioRxiv - Immunology 2024Quote: ... were isolated from spleens and lymph nodes of donor mice using magnetic cell separation (MACS) using mouse CD8a+ T cell isolation kits (Miltenyi Biotec), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-075), and 1 × 103 OT-I T cells in 100 mL of dPBS were transferred by retroorbital injection into recipient mice.
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bioRxiv - Physiology 2024Quote: ... Neonatal ventricular cardiomyocytes (NRVM) were obtained by enzymatic dissociation of 1-day-old Wistar rats heart using Neonatal Heart Dissociation Kit (Miltenyi Biotec). Ventricles were cut into 1-2 mm pieces and incubated with digestive enzymes (37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The flow through was collected and astrocytes were then acutely isolated using anti-ACSA-2+ (astrocyte cell surface antigen 2) Microbead Kit (Miltenyi Biotec). The flow through cell suspension was incubated in 15 µl of FcR blocking buffer for 10 min at 4°C followed by 15 µl of ACSA-2 microbeads for 10 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Then cortex and hippocampus parts were taken and dissociated into single cell suspension by using Adult Brain Dissociation Kit (Miltenyi Biotec) based on the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and each SEZ was cut into 5 or 6 small pieces of tissue and digested enzymatically using the Neural Tissue Dissociation Kit (T) in a GentleMACS Octo Dissociator with heating (Miltenyi Biotec). When the program was finished ...
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bioRxiv - Biochemistry 2024Quote: ... mouse cell depletion was performed on whole bone marrow cells using the Mouse Cell Depletion Kit and LS columns (Miltenyi Biotec). RIPA lysis was performed with purified human cells and 25µg of protein were loaded on 10% Bis-Tris-Gel NuPAGE gels with MOPS SDS running buffer (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Murine T cells were isolated from C57Bl6/J (WT or IFNγ-/-) splenocytes with CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25+ Tregs and CD4+CD25− T cells were purified from the peripheral LNs and spleen of Apoe−/− or Ccr4−/−Apoe−/− mice using a CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) and anti-CD4 beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... total CD4+ T cells or total CD8a+ T cells were isolated from WT or Aim2−/−mice using a magnetic cellular isolation kit (CD4+, Miltenyi Biotec, 130-104-454 ...
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bioRxiv - Immunology 2024Quote: ... B and Treg cells were further isolated by negative selection using the mouse Pan T cell isolation kit II (130-095-130; Miltenyi Biotec), the mouse Pan B cell isolation kit II (130-104-443 ...
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bioRxiv - Immunology 2024Quote: Tumors were mechanically dissociated with scissors and digested enzymatically using the Tumor Dissociation Kit mouse and the gentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The remaining cell suspensions were then resuspended in DMEM medium and enriched with viable cells using the Dead Cell Removal Kit magnetic sorting protocol (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: EpCAM-positive cells were isolated from the differentiated endodermal cells using a magnetic cell sorting kit (MACS; Miltenyi Biotec K.K. Cologne, Germany) with the CD326 (EpCAM ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions containing the IEC fraction were diluted to 108 cells/mL in MACs buffer (0.5% BSA 2mM EDTA) before being processed with the Miltenyi Dead Cell Removal Kit (Miltenyi Biotec, Auburn, CA) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein complexes with TTL3-GFP and free GFP as a control were isolated using the μMACS and MultiMACS GFP Isolation Kits (Miltenyi Biotec, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: NK cells were purified from PBMCs from healthy donors using an NK cell isolation kit (Miltenyi Biotec, Inc., San Diego, CA, USA). NK cells were stimulated with irradiated (100 Gy ...
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bioRxiv - Neuroscience 2020Quote: ... The brain tissues were cut into small pieces and dissociated into single cells using a modified version of the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec, US). Myelin debris was removed using the Myelin Removal kit II (130-096-733 ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested spleens from intramuscularly vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Spleen Dissociation Kit (Miltenyi Biotec) with slight modifications to the manufacturer’s protocol ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested lungs from intranasally vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Lung Dissociation Kit (Miltenyi Biotec) according to manufacturer’s instructions including RBC lysis ...
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bioRxiv - Microbiology 2021Quote: ... B lymphocyte isolation was conducted through negative selection by using the B cell Isolation Kit II (Miltenyi Biotec, Cat#130-091-151) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Lung tissue fragments were mechanically minced and dissociated with enzymes according to a Lung Dissociation Kit protocol (Miltenyi Biotec, 130-095-927). A Miltenyi Gentle MACS dissociator was used for mincing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... hiPSC-aCMs and -vCMs at day 20-30 post-differentiation were dissociated into single cells which were then enriched using a MidiMACS PSC-derived Cardiomyocyte Isolation Kit (Miltenyi Biotec, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Primary murine peritoneal cells were sorted into B-1a and B-1b cells using MACS B-1a selection kit (Miltenyi Biotec inc.). Sorted cells were cultured in RPMI1640 containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... We used a commercially available neural tissue dissociation kit for enzymatic dissociation following the manufacturer’s protocol (Miltenyi Biotec, San Diego, CA, USA). Specimens were subsequently washed with cold PBS ...
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bioRxiv - Immunology 2020Quote: ... Skin T cells from the breast and abdomen were isolated from punch biopsies following a standardized digestion protocol with the gentleMACS™ Dissociator and the whole skin dissociation kit containing enzyme A and D (both Miltenyi Biotec) before FACS-sorting for live (Hoechst 33258 negative or propidium iodide negative ...
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bioRxiv - Immunology 2021Quote: NK cells were purified by negative selection from mice splenocytes with an NK cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Granulocytes/neutrophils were isolated from the erythrocyte fraction by dextran sedimentation or from whole blood by immunomagnetic selection (130-104-434, MACSxpress® Whole Blood Neutrophil Isolation Kit; Miltenyi Biotec), and CD10neg/CD10pos neutrophils were separated by flow-cytometric sorting ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were separated from healthy controls PBMC using pan T cell isolation kit and magnetic-activated cell sorting (Miltenyi Biotec, USA). Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from splenocytes either by using the CD4+ or CD8+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s recommendation alone or followed by anti-CD4 ...
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bioRxiv - Immunology 2022Quote: γδ T cells were sorted from the spleen of MCMV infected or control TCRα-/- mice using the TCR γδ T cell Isolation kit (Miltenyi Biotec). For γδ TCM and TEM sorting ...
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bioRxiv - Neuroscience 2022Quote: ... via dounce homogenization and 30% Percoll gradient or enzymatically digested using mouse tumor dissociation kit and gentleMACS™ Octo Dissociator (Miltenyi Biotec). Spleens and lymph nodes were either digested with Collagenase IV/DNAse or not digested before mechanical disruption ...
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bioRxiv - Cancer Biology 2022Quote: ... Neutrophils and the other cell types were isolated from the suspension using the MACS Neutrophil isolation kit (Miltenyi Biotec, 130-097-658) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Epithelial cells were isolated from colon samples in the pre-digestion buffer using the Lamina Propria Dissociation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... A purified monocyte fraction was isolated from the thawed cells using the negative selection MACS® Pan Monocyte Isolation Kit (Miltenyi Biotec) with LS columns according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were dissected from the limb and dissociated by shaking for 45 min at 37°C in an enzyme mix from the tumor dissociation kit (Miltenyi Biotec, 130096730). Cell suspension was then strained through a 40 µm filter ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brains from Control and Serpina3n cKO mice were processed using the Neural Tissue Dissociation Kit (P) (Cat# 130-092-628, Miltenyi Biotec, Germany) in conjunction with the gentleMACS Dissociator (Cat# 130-093-235 ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...