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Citations for Miltenyi Biotec :
1751 - 1800 of 2165 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... of cytapheresis rings from healthy donors (EFS Bordeaux) and B cells were sorted with the B cell isolation kit II (Miltenyi Biotec). Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... through the New York Blood Center and CD4+ cells isolated by negative selection with a Miltenyi CD4 T cell isolation kit II (Miltenyi Biotec). Cell-free virus was produced by transfection of 293T cells in 10 cm plates using polyjet (Signagen) ...
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bioRxiv - Immunology 2020Quote: Cryogenically preserved healthy human peripheral blood T-cells or murine splenic T-cells were magnetically isolated by negative selection using Pan T-cell isolation kits from Miltenyi Biotec. Murine splenocytes underwent RBC lysis (Sigma ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were collected from spleens and lymph nodes of naïve CD45.1+ and CD45.1.2+ P14 TCR transgenic mice and negatively enriched for CD8+ T cells using the CD8a+ T Cell Isolation Kit and LS MACS Columns (Miltenyi Biotec). 1×106 CD8+ T cells were plated per well in 48 well flat-bottom plates ...
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bioRxiv - Microbiology 2020Quote: ... depleted of CD8+ T cells using a negative selection step (CD8 MicroBead Kit; LD columns, QuadMACS Separator, all from Miltenyi Biotec) per manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... mice were transcardially perfused with PBS and tissue dissociated with the Neural Tissue Dissociation kit (P) (Miltenyi Biotec, 130-092-628) in C-tubes (Miltenyi ...
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bioRxiv - Immunology 2020Quote: T cells were isolated from splenocytes of 8-12-week-old mice using a Pan T Cell Isolation Kit II (Miltenyi Biotec) with >90% purity ...
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bioRxiv - Neuroscience 2022Quote: Membrane potential measurements were made by impaling pericytes on microvessels isolated from Cspg4-DsRed mice using a papain-based Neural Tissue Dissociation kit (Miltenyi Biotec), as described previously21,25 ...
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bioRxiv - Immunology 2022Quote: ... The cell concentration and viability were determined using an automatic cell counter (Cellometer Auto 2000, Nexcelom Bioscience, Lawrence, MA, USA) and the Dead Cell Removal Kit (Miltenyi Biotec) was used to remove dead cells to obtain a cell viability of ≥80%.
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bioRxiv - Immunology 2022Quote: ... The tissues were mechanically dissociated and enzymatically digested by incubating at 37°C using enzymes from the Umbilical Cord Dissociation Kit (Miltenyi Biotec). A second round of mechanical dissociation was performed using the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: PCLS were dissociated as described above and dead cells were removed using Miltenyi Dead Cell Removal Kit (130-090-101, Miltenyi Biotec). For the multiplexing purpose ...
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bioRxiv - Cancer Biology 2022Quote: ... Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... We then purified CD4 CD25 and CD4+CD25− T cells using CD4+CD25+ Regulatory T Cell Isolation Kit (#130-091-301) and AutoMACS technology (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens of transplanted PyMT tumor-bearing mice with a CD8a+ T Cell Isolation Kit (#130-104-075, Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mm long and spanning the cortex depth but excluding the white matter) was microdissected and dissociated using the “Adult Brain Dissociation” kit from Miltenyi Biotec following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cells using dissociation kits according to the manufacturer’s protocol (preserving all surface epitopes, Miltenyi Biotec #130-095-927), then stained with APC-conjugated antibodies against CD45 (eBioscience #17-0451-83) ...
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were purified using a CD11b+ isolation kit per the manufacturer’s recommended protocol (Miltenyi Biotec, Auburn, CA; cat# 130-091-100). Prior to setting the culture ...
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bioRxiv - Immunology 2020Quote: Total CD4+ T cells from baseline leukapheresis were isolated from cryopreserved PBMCs by manual magnetic labeling and negative selection using the CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2021Quote: ... live CD45− cells were isolated by negative selection from digested WT and CCN4-KO tumors using the mouse CD45 (TIL) MicroBeads kit (Miltenyi Biotec). A total of 1 ×104 cells/well were cultured for 36 h in DMEM supplemented with 10% dialyzed FBS (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... and from CD45− cells isolated from WT and CCN4-KO tumors by negative selection using a mouse CD45 MicroBeads kit (Miltenyi Biotec). Conditioned media were obtained by culturing 1 ×104 CD45− cells/well for 36 h in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from PBMCs by a magnetic bead “negative” isolation procedure using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... Tumor and normal tissue samples harvested from euthanized mice were collected into ice-cold PBS and subsequently processed using a murine tumor digestion kit (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937, respectively, Miltenyi Biotec) (Chucair-Elliott et al. ...
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bioRxiv - Physiology 2021Quote: Muscle leukocytes were isolated from hamstring muscles four days post-surgery using a skeletal muscle dissociation kit (Miltenyi Biotech, cat#130-098-305) and GentleMACS Dissociator tissue homogenizer (Miltenyi Biotec) as previously described (19) ...
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bioRxiv - Developmental Biology 2021Quote: ... We subjected approximately 1g minced dissected villous tissue to the Miltenyi Tumor Dissociation Kit on the GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) to yield single-cell suspensions of viable placental cells in 5μM StemMACS™ Y27632 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: B cells were isolated from spleens of WT female mice and enriched with a B cell isolation kit (Miltenyi Biotec MACS). The purity of isolated B cells was 96±0.8% ...
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bioRxiv - Neuroscience 2021Quote: ... Primary neurons where derived from neural tissue following standard operational procedure from p0-1 mice brain using the Neural Tissue Dissociation Kit-Postnatal neurons (Cat. 130-094-802, Miltenyi Biotec). After removing the meninges ...
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bioRxiv - Developmental Biology 2020Quote: ... A region spanning from the subpallium to the DP (see Fig. 1A) was dissected on both hemispheres and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) and a gentleMACS Octo Dissociator with Heaters following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Human neutrophils were isolated from peripheral blood by negative selection with magnetic microspheres MACSxpress Neutrophil Isolation Kit and MACSxpress Separator (Miltenyi Biotec), according to the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... Conventional T cells were enriched from FVB/N mouse spleen single-cell suspensions using the mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Primary murine CD4 T cells were isolated from the spleens and lymph nodes of mice using a negative isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Responder CD8 T cells were isolated from the spleens and lymph nodes of mice using a negative isolation kit (Miltenyi Biotec) and stained with 5μM CellTrace Violet (CTV ...
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bioRxiv - Immunology 2021Quote: ... B cell isolation from cryopreserved PBMCs was carried out using Memory B cells Isolation Kit (MACS/Miltenyi Biotec, Bergisch Gladbach, Germany). Next ...
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bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
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bioRxiv - Immunology 2020Quote: ... PolyPeptide group) were co-cultured with 105 OTII CD4+ T cells (purified with a CD4+ T cell isolation kit, Miltenyi Biotec) in RPMI medium (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: Bulk polyclonal CD8+ T cells were isolated from the spleen and lymph nodes of WT or Dct-/- mice using negative magnetic enrichment (CD8a+ T cell Isolation Kit; Miltenyi Biotec). Enriched CD8+ T cells (typically ∼75-90% pure ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T-cells were isolated from lymph nodes and spleens of OT-II TCR transgenic mice using negative selection with a mouse CD4+ T Cell Isolation Kit (Miltenyi Biotec). Splenocytes from C57BL/6 mice were T cell depleted by complement lysis of Thy1.2+ cells (clone J1J ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using magnet activated cell sorting (MACS) using the Pan T cell Isolation Kit (negative selection) according to the manufacturer’s instructions (Miltenyi Biotec, Germany). Following separation ...
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bioRxiv - Cell Biology 2020Quote: Single intestinal cells were obtained after enzymatic dissociation of the tunica muscularis using a Tumor Dissociation Kit (Miltenyi Biotec, Woking, UK), and YFP+ cells isolated using fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then negatively selected for CD45 and positively selected for CD117 (c-kit) using magnetic bead separation (Miltenyi Biotec). After selection ...
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bioRxiv - Immunology 2021Quote: ... and microglia was isolated with the Papain-based Neural Tissue Dissociation Kit (P) (# 130-092-628, Miltenyi Biotec B.V. & Co. KG) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with the LIVE/DEAD Fixable Aqua Dead Cell Staining kit (ThermofisherScientific) and acquired on a MACS Quant (Miltenyi Biotec) cytometer ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions obtained from spleens were processed with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec; Cat#: 130-104-075) and transferred via the tail vein into recipient mice.