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Citations for Miltenyi Biotec :
1801 - 1850 of 2522 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion and tissue dissociation of the left lateral liver lobe was performed using a GentleMACS Octo Dissociator with heaters and the liver perfusion kit for mice according to the manufacturer’s protocol (Miltenyi Biotec). Hepatocytes were separated from lymphocytes by centrifugation at 50× g for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells were then obtained using a negative magnetic bead-based selection kit for pan T-cell isolation following the manufacturer’s protocol (Miltenyi Biotec). A negative selection was chosen to minimize any activation of T-cells during the selection process ...
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bioRxiv - Developmental Biology 2023Quote: ... The flow-through was then subjected to MuSC enrichment using the satellite cell isolation kit (Miltenyi Biotec 130-104-268). Once each population was isolated ...
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bioRxiv - Microbiology 2023Quote: ... Cells from the colonic lamina propria (cLP) were isolated using a Lamina Propria Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) on the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD4 naïve T cells were isolated from pooled lymph nodes and spleens using the naïve CD4 T cell isolation kit (130-104-453, Miltenyi Biotec) and stained with BioTracker 655 Red Cytoplasmic Membrane Dye (SCT108 ...
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bioRxiv - Cancer Biology 2023Quote: Tumor samples harvested from mouse mammary glands were dissociated using the Mouse Tumor Dissociation Kit (Miltenyi Biotec,130-096-730), gentleMACS C tubes (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were strained (40 μm filters) and dead cells removed using a dead cell removal kit according to the manufacturer’s instructions (Miltenyi Biotec). Cell viability was determined using trypan blue staining and cells were counted using a haemocytometer ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...
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bioRxiv - Genomics 2023Quote: All other solid organs were dissociated into single-cell suspensions using Miltenyi’s Liver Dissociation Kit (Miltenyi Biotec, 130-105-807) with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from mouse spleen and lymph nodes by magnetic bead sorting using the Naïve CD4+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Cecal and colonic lamina propria cells were extracted using the Lamina Propria Dissociation Kit and gentleMACS Dissociators (Miltenyi Biotec, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: T-cells were isolated from healthy donor PBMCs using the Pan T-cell Isolation Kit following manufacturer’s instructions (Miltenyi Biotec). Isolated T-cells were activated using anti-CD3/CD28 antibody-coated Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and the supernatant was discarded while PMs were purified according to manufacturer’s instruction using a magnet purification kit by selecting F4/80+ cells (Miltenyi Biotec; Macrophage Isolation Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... were cut into small pieces and digested with in 2.4 mL of dissociation solution [DMEM medium supplemented with enzyme mixture (100ul Enzyme D, 50uL Enzyme R and 12.5uL Enzyme A)] using Tumor Dissociation Kit (Miltenyi Biotec). After incubation for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and B-CLL cells were further purified using the Pan B-cell Isolation Kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Versalyse buffer (Beckman-Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic separation with the CD34 Microbead Kit (Miltenyi Biotec, San Diego, CA, USA) and further purified by flow cytometric sorting as described below.
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bioRxiv - Cancer Biology 2023Quote: ... macrodissected HCC nodules and control liver samples were dissociated as single-cell suspensions using the Liver Dissociation kit (Miltenyi Biotec) and the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were enriched from bone marrow using a negative selection immuno-magnetic isolation kit according to the manufacture’s protocol (Miltenyi Biotec). Enriched neutrophils were suspended in PBS with 1% FBS and incubated with mouse FC block at a concentration of 1 µg/million cells for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells from the spleen of Foxp3-mRFP mice were enriched using a CD4 negative selection kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were magnetically labelled with Dead Cell Removal Microbeads from the Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101). For 1.0x107 cells ...
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bioRxiv - Immunology 2024Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 3h ...
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bioRxiv - Cancer Biology 2024Quote: ... hSS2 mouse tumors were harvested and digested to single cell suspension with the Miltenyi mouse tumor dissociation kit (Miltenyi Biotec). Approximately 5-10 million cells were crosslinked with 1% of formaldehyde ...
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bioRxiv - Biochemistry 2024Quote: We purified CD4+CD25-CD44lowCD62Lhigh naïve T cells obtained from the spleens of WT and Cars2+/– littermates with the Naïve CD4+ T Cell Isolation Kit II (130-093-227; Miltenyi Biotec) and an autoMACS Pro Cell Separator (Miltenyi Biotech) ...
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bioRxiv - Immunology 2024Quote: ... then mechanically dissociated in enzyme mix for 30 min at 37°C following the manufacturer’s protocol (Lung Dissociation Kit, gentleMACS Tissue Dissociator, Miltenyi Biotec). The single cell suspension was applied to a 70 µm filter with 2.5 mL Separation Buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The cell dissociation procedure followed the guidelines of the Neural Dissociation Kit (T) (130-093-231, Miltenyi Biotec GmbH, Germany). After filtering with medium with 10 % (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... mice were transcardially perfused with PBS and tissue dissociated with the Neural Tissue Dissociation kit (P) (Miltenyi Biotec, 130-092-628) in C-tubes (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... NK cells in the spleen were isolated and purified by using mouse NK cell isolation kit II (130-096-892, Miltenyi Biotec). The purity of NK cells was analyzed by FACS ...
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bioRxiv - Immunology 2021Quote: ... the negative cells fraction were further selected for the CD8+ positive cells (CD8a+ T Cell Isolation Kit, mouse, 130-104-07, Miltenyi Biotec). For the tetramers binding reaction ...
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bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS) T-cell subset Isolation Kits (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD4+ and CD8+ cells according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were minced with a scalpel and then enzymatically digested with a Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929, Germany) on a gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... pelleted splenocytes isolated from mice spleen were resuspended in FACS for NK cells purification with Mouse NK Cell Purification Kit (Miltenyi Biotec) according to the ...
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bioRxiv - Immunology 2021Quote: ... naïve B cells were isolated from the spleens of mice using the B cell isolation kit (Miltenyi Biotec: 130-090-862) and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final ...
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bioRxiv - Immunology 2021Quote: ... dead cells were removed from our 24h activation sample (∼50% viability) using a MACS® Dead Cell Removal Kit (Miltenyi Biotec). In brief ...
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bioRxiv - Genomics 2020Quote: ... Brain slices were dissociated mechanically using a gentleMACS™ Octo Dissociator and enzymatically using the Adult Brain Dissociation kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... Cell suspensions were then filtered through 70-µm cell strainers (Falcon) and depleted of apoptotic and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... Para-tumour lung tissue samples from metastatic cancer or fibrosis patients were processed using a tumour dissociation kit (MACS Miltenyi Biotec) to isolate immune and epithelial cells ...
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bioRxiv - Pathology 2019Quote: ... Increased ratios of MDSCs and nonMDSCs were co-cultured with splenic T cells isolated with Pan T Cell Isolation Kit II (Miltenyi Biotec) and stained with 1 μM Carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Bioengineering 2019Quote: ... The monocytic cell fraction was isolated from the hPBMC fraction via negative selection using the MACS pan-monocyte isolation kit (Miltenyi Biotec) according to the supplier’s instructions ...