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Citations for Miltenyi Biotec :
1701 - 1750 of 2522 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: CD4+ T cells were pre-enriched from spleens of CD45.1 or CD45.1/2 OT-II mice by negative selection using the CD4+ T-cell isolation kit (Miltenyi Biotec). Live (SytoxBlue−)CD25− CD44loCD62L+TCRβ+vα2+ naïve OT-II T cells were FACS-isolated ...
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bioRxiv - Immunology 2024Quote: ... Purification of primary pDC was performed using the Plasmacytoid Dendritic Cell Isolation Kit II (#130-097-415 Miltenyi Biotec), reaching >93% of purity ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 ng/mL granulocyte macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec). Primary cell cultures were maintained in the presence of mouse embryonic fibroblasts (STO ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated with 1 μg/ml CpG oligodeoxyribonucleotide 2006 (Miltenyi Biotec), 2.4 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry was done using anti-CD133/1-PE (Miltenyi Biotec, clone AC133) and anti-GD2-APC (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... Cell purity was assessed by anti-GLAST (ACSA-1) phycoerythrin antibody (Miltenyi Biotec) using flow cytometry (CyanADP Cytometer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and anti-cMyc-FITC (1:50, Miltenyi Biotec, Somerville, MA, #130-116-485) at 4°C for 45 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-cardiac troponin-T-APC (Miltenyi Biotec, 130-120-403, 1:50) in blocking buffer (PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and anti-cMyc-FITC (1:50, Miltenyi Biotec, Somerville, MA, #130-116-485) at 4°C for 45 min in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... RRID:AB_1227553) or anti- granulocytes (1:100, Miltenyi Biotec Cat# 130-108-084, RRID:AB_2651886), PerCP5.5-conjugated anti-CD8 (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Anti-foam Y-30) in M-tubes (gentleMACS™ Tubes, Miltenyi Biotec). Organs were then mechanically homogenized using a gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2023Quote: ... and APC-conjugated anti-ACSA-2 (Miltenyi Biotec, 130-116-245, 1:100) antibodies for 30 min at 4℃ ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-MHC-II (1:100, Miltenyi Biotec Cat# 130-107-822, RRID:AB_2652895), andFITC-conjugated anti-granulocytes (1:100 Miltenyi Biotec Cat# 130-108-084 ...
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bioRxiv - Neuroscience 2024Quote: ... andFITC-conjugated anti-granulocytes (1:100 Miltenyi Biotec Cat# 130-108-084, RRID:AB_2651886) for 15 minutes at 4°C in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-mouse CD45-PE (1:50 dilution; 130-110-797, Miltenyi Biotec) for 10 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... diluted at 1:16 in MACS buffer (130-091-221, Miltenyi Biotec, USA) at 4°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... washed with 1x PBS and were treated with 1% Fc block (Miltenyi Biotec) in 1x PBS for 10 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... WBM was depleted of terminally differentiated hematopoietic cells using a murine Lineage Cell Depletion Kit (Miltenyi Biotec 130-090-858) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: Lineage negative and CD31 positive BMCs were isolated using first immunomagnetic Lineage Cell Depletion Kit (130-092-211, Miltenyi Biotec) followed by positive selection with CD31 MicroBead Kit (130-091-935 ...
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bioRxiv - Immunology 2021Quote: ... The uncollected non-myeloid (CD11b-) cells were processed with the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-095-130) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Day 22 EMLOs (~25 count) were pooled and dissociated using the Neural Tissue Dissociation Kit (P, palpain) according to manufacturer’s instructions (Miltenyi Biotec) and similarly to previous reports44 ...
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bioRxiv - Cancer Biology 2020Quote: Tumors were dissected and minced following the manufacturer’s protocol using MACS C tubes and the mouse Tumor Dissociation Kit (Miltenyi Biotec). After tumor dissociation ...
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bioRxiv - Genomics 2020Quote: ... poly-A RNA was isolated from 60∼90ug total RNA using μMACS mRNA Isolation Kits (Miltenyi Biotec Inc. CA, USA). Poly-A RNA (500 ng ...
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bioRxiv - Immunology 2019Quote: ... Bone marrow cells were MACS enriched for c-Kit+ cells using CD117 MicroBeads Ultrapure (Miltenyi Biotec cat #130-091-224) according to manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... Cells were washed and labeled for separation by negative selection using a MACS pan-dendritic cell isolation kit (Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: ... Murine B cells were enriched by negative selection of IgMpos cells with a mouse B cell isolation kit (Miltenyi Biotec, #130-090-862 ...
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bioRxiv - Molecular Biology 2019Quote: ... Lineage negative cells were collected by applying the Direct Lineage Cell Depletion Kit mouse (130-090-858, Miltenyi Biotec GmbH) and magnetic separation following manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... CD34 positive cells were then bead enriched using the CD34 MicroBead Kit UltraPure (Miltenyi Biotec, catalog number 130-100-453) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Allogenic naïve CD4+ T cells and CD8+ T cells were isolated from buffycoats using the naïve CD4+ T cell Isolation kit II (Miltenyi Biotec) and CD8+ T cell Isolation kit (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2020Quote: ... Single cell suspensions were generated using the Neural Tissue Dissociation Kit – Postnatal Neurons (Miltenyi Biotec. Cat no. 130-094-802), following manufacturer’s instructions for manual dissociation ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspension was obtained by automated dissociation using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat#130-092-628) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2020Quote: ... Cardiac cells were isolated by excluding dead cells using the MACS dead cell removal kit (Miltenyi Biotec, 130-090-101) and were suspended in the optimized culture medium for cardiac cell culture ...
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bioRxiv - Neuroscience 2020Quote: EV preparation by immunobead isolation was performed using the Pan (mixture of CD9-, CD63- and CD81)-Exosome Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec). 2 ml of Amicon Ultra-15 (100 kDa cutoff ...
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bioRxiv - Cancer Biology 2020Quote: ... polyA+ RNA was purified using the 96-well MultiMACS mRNA isolation kit on the MultiMACS 96 separator (Miltenyi Biotec, Germany) from total RNA with on column DNaseI-treatment as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the brain tissues (cortices and hippocampi) were enzymatically dissociated with Neural Tissue Dissociation Kit-P (#130-092-628; Miltenyi Biotec). Myelin was removed by MACS Myelin Removal Beads II (#130-096-733 ...
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bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... In vivo infections were performed by intranasal injection of 5×10^5 bacteria per mouse Lungs were collected after 20h from infection and digested using the Lung Dissociaton Kit according to the manufacturer’s instructions (Miltenyi Biotec) plus added Dispase II at 0,1U/ml (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... tumors (0.5 g) were mechanically disrupted by chopping and then chemically digested using Tumor Dissociation Kit (130-096-730, Miltenyi Biotec) and gentleMACS Dissociator (130-096-427 ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells were purified via negative selection using the Naïve CD4+ T cell Isolation mouse Kit (Miltenyi Biotec) according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
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bioRxiv - Molecular Biology 2022Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2022Quote: ... P14 and P21 mice cortices during an additional step using the debris removal kit (Miltenyi Biotec; ref 130-090-101). Cells were suspended in a 0.5% NGS solution then incubated with anti-PDGFRα or anti-O4 coupled-beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... total CD4+ T cells were directly isolated from buffy coats using the StraightFrom Buffy Coat CD4 MicroBead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Immunology 2022Quote: Neutrophils were purified from freshly isolated mouse bone marrow cell suspensions using an autoMACS pro-selector cell separator with a MACS neutrophil isolation kit for negative selection in accordance with the manufacturer’s instructions (Miltenyi Biotec). Neutrophils were aged overnight in serum-free medium at 3 × 106 cells/ml at 37 °C and 5 % CO2 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2020Quote: ... For the isolation of pDCs from PBMCs the Plasmacytoid Dendritic Cell Isolation Kit II from Miltenyi Biotec (# 130-097-415) was used ...