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Citations for Miltenyi Biotec :
1801 - 1850 of 2473 citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... The monocytic cell fraction was isolated from the hPBMC fraction via negative selection using the MACS pan-monocyte isolation kit (Miltenyi Biotec) according to the supplier’s instructions ...
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bioRxiv - Immunology 2019Quote: B220+ B cells were recovered from bone marrow mononuclear cells from WT or CCR7−/− mice by negative selection using a MACS microbeads isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Autologous or allogenic CD3+ T cells used in T cell co-culture experiments were isolated using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instruction.
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... cells were purified with magnetic cell sorting (MACS) from healthy donor PBMCs using the CD1c (BDCA1) DC isolation kit (Miltenyi Biotec). To obtain a CD1c+CD14- population ...
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bioRxiv - Immunology 2021Quote: ... Untouched peripheral blood cDC1 and cDC2 (cDCs) and pDCs were obtained from PBMC after negative immunomagnetic separation with the Myeloid Dendritic Cell Isolation kit (Miltenyi Biotec) and the Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2020Quote: ... the population of IFNγ secreting cells was identified using the IFNγ Secretion assay detection kit (PE) (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral neutrophils were isolated by negatively removing all contaminating cells using the MACSxpress Neutrophil Isolation Kit (Miltenyi Biotec, 130-104-434) and BD Pharm Lyse buffer (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumor-bearing hemispheres and corresponding hemispheres from naïve animals were dissociated enzymatically to obtain a single-cell suspension with a Neural Tissue Dissociation Kit with papain (Miltenyi Biotec) or 0.5 mg/mL DNase I (DN25 ...
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bioRxiv - Neuroscience 2020Quote: ... P1 and P2 were re-suspended in PBS and myelin was depleted from both fractions according to the instructions of a commercial myelin-removal kit (Miltenyi Biotec), before sorting MF-CA3 synaptosomes from myelin-depleted P1.
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bioRxiv - Neuroscience 2020Quote: Micro-dissected dentate gyri were cut into pieces with a scalpel blade and enzymatically dissociated using the Neural Tissue Dissociation Kit P (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were processed as previously described 54 and CD4+ T cells were enriched with the CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Mouse CD8a+ isolation kit (Cat no.-130-104-075) and Mini Separator Columns (Cat no.-130-042-201) were purchased from Miltenyi Biotec. H-2Kb OVA Tetramer-SIINFEKL-PE (Cat no.-TS-5001-1C ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Monocyte-and lymphocyte-enriched PBMC suspensions were sorted using Isolation kits and Depletion Column Type LS (Miltenyi Biotec Inc., Auburn, CA) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells were isolated by ficoll density gradient centrifugation (Dakewe) and T cells were purified using the Pan T Cell Isolation Kit (Miltenyi Biotec), and stimulated with CD3/CD28 T cell Activator Dynabeads (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells were resuspended with 100 µl of Inside Perm Buffer (Inside Stain Kit, Miltenyi Biotec). Eight µl of anti-GFAP and 2.5 µl of anti-Iba1 were added to the cell suspensions and mixed by gently pipetting ...
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bioRxiv - Cell Biology 2022Quote: ... The CD8+ T cells were purified from PBMCs using negative selection bead binding (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec). The following compounds were used at the indicated concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... The piece of cerebellum was dissociated into a single cell suspension by a gentleMACS Octo Dissociator within a gentleMACS C Tube in combination with the Brain Tumor Dissociation Kit (P) (Miltenyi Biotec) following all associated manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... autologous T cells were isolated from patient NSCLC lesions or from healthy donor PBMCs using the Pan T-cell Isolation Kit (Miltenyi Biotec). Autologous T cells were co-cultured with either ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue of single mice was digested with trypsin and DNase according to the guidelines of the Neural Tissue Dissociation Kit (T) (Miltenyi Biotec) using a Gentle MACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533 ...
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bioRxiv - Immunology 2020Quote: ... the SILP and the CLP single cell suspensions were obtained using the Lamina Propria Dissociation Kit (Miltenyi Biotec SAS, Paris, France) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... of cytapheresis rings from healthy donors (EFS Bordeaux) and B cells were sorted with the B cell isolation kit II (Miltenyi Biotec). Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... through the New York Blood Center and CD4+ cells isolated by negative selection with a Miltenyi CD4 T cell isolation kit II (Miltenyi Biotec). Cell-free virus was produced by transfection of 293T cells in 10 cm plates using polyjet (Signagen) ...
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bioRxiv - Neuroscience 2019Quote: ... brain stem and cerebellum were removed and the remaining tissue (cerebrum) was freed from meninges and dissociated by enzymatic digestion using a Neural Tissue Dissociation Kit P (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2020Quote: Cryogenically preserved healthy human peripheral blood T-cells or murine splenic T-cells were magnetically isolated by negative selection using Pan T-cell isolation kits from Miltenyi Biotec. Murine splenocytes underwent RBC lysis (Sigma ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were collected from spleens and lymph nodes of naïve CD45.1+ and CD45.1.2+ P14 TCR transgenic mice and negatively enriched for CD8+ T cells using the CD8a+ T Cell Isolation Kit and LS MACS Columns (Miltenyi Biotec). 1×106 CD8+ T cells were plated per well in 48 well flat-bottom plates ...
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bioRxiv - Microbiology 2020Quote: ... depleted of CD8+ T cells using a negative selection step (CD8 MicroBead Kit; LD columns, QuadMACS Separator, all from Miltenyi Biotec) per manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... mice were transcardially perfused with PBS and tissue dissociated with the Neural Tissue Dissociation kit (P) (Miltenyi Biotec, 130-092-628) in C-tubes (Miltenyi ...
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bioRxiv - Immunology 2020Quote: T cells were isolated from splenocytes of 8-12-week-old mice using a Pan T Cell Isolation Kit II (Miltenyi Biotec) with >90% purity ...
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bioRxiv - Neuroscience 2022Quote: Membrane potential measurements were made by impaling pericytes on microvessels isolated from Cspg4-DsRed mice using a papain-based Neural Tissue Dissociation kit (Miltenyi Biotec), as described previously21,25 ...
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bioRxiv - Immunology 2022Quote: ... The cell concentration and viability were determined using an automatic cell counter (Cellometer Auto 2000, Nexcelom Bioscience, Lawrence, MA, USA) and the Dead Cell Removal Kit (Miltenyi Biotec) was used to remove dead cells to obtain a cell viability of ≥80%.
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bioRxiv - Immunology 2022Quote: ... The tissues were mechanically dissociated and enzymatically digested by incubating at 37°C using enzymes from the Umbilical Cord Dissociation Kit (Miltenyi Biotec). A second round of mechanical dissociation was performed using the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: PCLS were dissociated as described above and dead cells were removed using Miltenyi Dead Cell Removal Kit (130-090-101, Miltenyi Biotec). For the multiplexing purpose ...
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bioRxiv - Immunology 2022Quote: ... We then purified CD4 CD25 and CD4+CD25− T cells using CD4+CD25+ Regulatory T Cell Isolation Kit (#130-091-301) and AutoMACS technology (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: The tissue pieces were rapidly transferred into the gentleMACS C Tube containing Human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens of transplanted PyMT tumor-bearing mice with a CD8a+ T Cell Isolation Kit (#130-104-075, Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Organs were cut into smaller pieces with a scalpel and incubated for 30 minutes with reagents from a tumour dissociation kit (Order no. 130-096-730, Miltenyi Biotec). Cells were strained through a 70 μm nylon mesh using the blunt end of a syringe to make a single cell suspension ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mm long and spanning the cortex depth but excluding the white matter) was microdissected and dissociated using the “Adult Brain Dissociation” kit from Miltenyi Biotec following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cells using dissociation kits according to the manufacturer’s protocol (preserving all surface epitopes, Miltenyi Biotec #130-095-927), then stained with APC-conjugated antibodies against CD45 (eBioscience #17-0451-83) ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were purified using a CD11b+ isolation kit per the manufacturer’s recommended protocol (Miltenyi Biotec, Auburn, CA; cat# 130-091-100). Prior to setting the culture ...
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bioRxiv - Immunology 2020Quote: Total CD4+ T cells from baseline leukapheresis were isolated from cryopreserved PBMCs by manual magnetic labeling and negative selection using the CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from PBMCs by a magnetic bead “negative” isolation procedure using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and LS columns (Miltenyi Biotec) ...