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Citations for Miltenyi Biotec :
1751 - 1800 of 2473 citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... NK cells were then re-purified by negative selection using purification kit and magnetic columns according to manufacturer’s instructions to reach purities of approximately 98% (Miltenyi Biotec, MS columns and isolation kit ref no.130-115-818) ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8 T cells were isolated from the spleen of pmel mice (having CD8 T cells bearing T cell receptors specific for the gp100 antigen) by magnet-assisted cell sorting (Naïve CD8+ T cell isolation kit, Miltenyi Biotec) and stained with Cell Trace Violet (CTV ...
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bioRxiv - Molecular Biology 2023Quote: ... Hearts were mashed with pestles for 2 min and cell isolation performed according to manufacturer’s instructions (Neonatal Heart Dissociation Kit, Miltenyi Biotec). Cell suspension was centrifuged for 5 min at 600 x g and cells were resuspended in cell culture medium and plated in 22.1 mm plates ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated into a single cell suspension using a neural tissue dissociation kit (Miltenyi Biotec; 130-092-628). Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD4 naïve T cells were isolated from pooled lymph nodes and spleens using the naïve CD4 T cell isolation kit (130-104-453, Miltenyi Biotec) and stained with BioTracker 655 Red Cytoplasmic Membrane Dye (SCT108 ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells were then obtained using a negative magnetic bead-based selection kit for pan T-cell isolation following the manufacturer’s protocol (Miltenyi Biotec). A negative selection was chosen to minimize any activation of T-cells during the selection process ...
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bioRxiv - Microbiology 2023Quote: ... Cells from the colonic lamina propria (cLP) were isolated using a Lamina Propria Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) on the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The flow-through was then subjected to MuSC enrichment using the satellite cell isolation kit (Miltenyi Biotec 130-104-268). Once each population was isolated ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion and tissue dissociation of the left lateral liver lobe was performed using a GentleMACS Octo Dissociator with heaters and the liver perfusion kit for mice according to the manufacturer’s protocol (Miltenyi Biotec). Hepatocytes were separated from lymphocytes by centrifugation at 50× g for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec) into complete RPMI media ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were strained (40 μm filters) and dead cells removed using a dead cell removal kit according to the manufacturer’s instructions (Miltenyi Biotec). Cell viability was determined using trypan blue staining and cells were counted using a haemocytometer ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Genomics 2023Quote: All other solid organs were dissociated into single-cell suspensions using Miltenyi’s Liver Dissociation Kit (Miltenyi Biotec, 130-105-807) with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Cell Biology 2023Quote: CD34+ magnetic cell isolation was performed on the PBMCs according to the manufacturer’s protocol for the Direct CD34+ progenitor cell isolation kit (Miltenyi Biotec), to enrich for haematopoietic progenitor cells ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were enriched from bone marrow using a negative selection immuno-magnetic isolation kit according to the manufacture’s protocol (Miltenyi Biotec). Enriched neutrophils were suspended in PBS with 1% FBS and incubated with mouse FC block at a concentration of 1 µg/million cells for 10 minutes on ice ...
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bioRxiv - Microbiology 2023Quote: Cecal and colonic lamina propria cells were extracted using the Lamina Propria Dissociation Kit and gentleMACS Dissociators (Miltenyi Biotec, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2023Quote: ... were cut into small pieces and digested with in 2.4 mL of dissociation solution [DMEM medium supplemented with enzyme mixture (100ul Enzyme D, 50uL Enzyme R and 12.5uL Enzyme A)] using Tumor Dissociation Kit (Miltenyi Biotec). After incubation for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and B-CLL cells were further purified using the Pan B-cell Isolation Kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Versalyse buffer (Beckman-Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... macrodissected HCC nodules and control liver samples were dissociated as single-cell suspensions using the Liver Dissociation kit (Miltenyi Biotec) and the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic separation with the CD34 Microbead Kit (Miltenyi Biotec, San Diego, CA, USA) and further purified by flow cytometric sorting as described below.
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bioRxiv - Biochemistry 2023Quote: Human primary monocytes were isolated from healthy donors using the autoMACS Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... The cell dissociation procedure followed the guidelines of the Neural Dissociation Kit (T) (130-093-231, Miltenyi Biotec GmbH, Germany). After filtering with medium with 10 % (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... + 10 U/μL DNase I DNase I (Roches)) or MACS Human Tumor Dissociation kit (DS_130-095-929, Miltenyi Biotec, Germany). The incubation was terminated when the digestive fluid turned turbid and the tissue block disappeared ...
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bioRxiv - Biochemistry 2024Quote: We purified CD4+CD25-CD44lowCD62Lhigh naïve T cells obtained from the spleens of WT and Cars2+/– littermates with the Naïve CD4+ T Cell Isolation Kit II (130-093-227; Miltenyi Biotec) and an autoMACS Pro Cell Separator (Miltenyi Biotech) ...
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bioRxiv - Immunology 2024Quote: ... then mechanically dissociated in enzyme mix for 30 min at 37°C following the manufacturer’s protocol (Lung Dissociation Kit, gentleMACS Tissue Dissociator, Miltenyi Biotec). The single cell suspension was applied to a 70 µm filter with 2.5 mL Separation Buffer ...
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bioRxiv - Immunology 2024Quote: ... and negative magnetic selection was then used to purify CD4+ T cells (CD4 T cell isolation Kit, human, Miltenyi Biotec). Isolated CD4+ T cells were plated at a density of 0.8x106/ml in AIM media supplemented with CTS Immune Cell SR (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were magnetically labelled with Dead Cell Removal Microbeads from the Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101). For 1.0x107 cells ...
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bioRxiv - Neuroscience 2021Quote: ... mice were transcardially perfused with PBS and tissue dissociated with the Neural Tissue Dissociation kit (P) (Miltenyi Biotec, 130-092-628) in C-tubes (Miltenyi ...
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Immunology 2021Quote: ... for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS) T-cell subset Isolation Kits (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD4+ and CD8+ cells according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were minced with a scalpel and then enzymatically digested with a Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929, Germany) on a gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... naïve B cells were isolated from the spleens of mice using the B cell isolation kit (Miltenyi Biotec: 130-090-862) and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final ...
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bioRxiv - Immunology 2021Quote: ... dead cells were removed from our 24h activation sample (∼50% viability) using a MACS® Dead Cell Removal Kit (Miltenyi Biotec). In brief ...
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bioRxiv - Genomics 2020Quote: ... Brain slices were dissociated mechanically using a gentleMACS™ Octo Dissociator and enzymatically using the Adult Brain Dissociation kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... Cell suspensions were then filtered through 70-µm cell strainers (Falcon) and depleted of apoptotic and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... Para-tumour lung tissue samples from metastatic cancer or fibrosis patients were processed using a tumour dissociation kit (MACS Miltenyi Biotec) to isolate immune and epithelial cells ...
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bioRxiv - Pathology 2019Quote: ... Increased ratios of MDSCs and nonMDSCs were co-cultured with splenic T cells isolated with Pan T Cell Isolation Kit II (Miltenyi Biotec) and stained with 1 μM Carboxyfluorescein succinimidyl ester (CFSE ...