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Citations for Miltenyi Biotec :
1801 - 1850 of 1888 citations for Human IgM Anti SARS CoV 2 Spike S1 Antibody CR3022 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Lineage depletion for CD4/TCRβ/TCRγδ/CD11c/Gr1/NK1.1/Ter119/Igκ was performed using MACS anti- PE Micr-beads (Miltenyi Biotec). CD19+Igλ+CD45.1+CD45.2− (WT ...
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bioRxiv - Plant Biology 2022Quote: ... Co-immunoprecipitation analyses were carried out as described recently using anti-GFP MicroBeads (Miltenyi Biotec, Germany; (Henrichs et al., 2012)) except that bands of interest were size-selected by Flamingo fluorescent gel stain (BioRad ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ expressing thymocytes were depleted using anti-CD4 and CD8 magnetic-activated cell-sorting (MACS) beads (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 minutes at 4°C in the dark ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Cancer Biology 2021Quote: ... and cocultures were separated with the optimized amount of Anti-Fibroblast MicroBeads (40 μL, for up to three 100 mm Petri Dishes, Miltenyi Biotec) according to the manufacturer’s protocol to reach minimal contamination in each compartment (Fig ...
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bioRxiv - Immunology 2020Quote: CD4+ cells from the spleen of mice were purified using LS positive selection magnetic-activated columns (MACs) and anti-CD4 (L3T4) beads (Miltenyi Biotec). CD4+ MACs sorted cells were further purified by fluorescence activated cell sorting using a FACS Aria (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: The immunomagnetic isolation of oligodendrocytes from Stag2f/y and Stag2f/y;Cre P12-P14 pups was conducted using anti-O4 microbeads (Miltenyi Biotec) according to a published protocol (Flores-Obando et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Monocytes were isolated from PBMCs using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (130-050-201; Miltenyi Biotec). CD14-positive monocytes were differentiated in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 20 min at RT with 250 μL of the Inside Fix solution then washed and stained in 100 μL of the Inside Perm solution containing PE anti-TNF (clone cA2, Miltenyi Biotec) and APC anti-IL-1β (clone REA1172 ...
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bioRxiv - Developmental Biology 2021Quote: ... Stem cell marker expression was validated in isolated cell lines by flow cytometry using anti-SSEA4-FITC (Cat. # 130-098-371, Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
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bioRxiv - Cancer Biology 2019Quote: ... Fresh BM aspirates from MM patients were routinely depleted from malignant plasma cells using anti-CD138-coated magnetic beads (Cat. #130051301 Miltenyi Biotec) and used for RNA sequencing and functional assays.
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bioRxiv - Immunology 2021Quote: ... were obtained by density gradient centrifugation and monocytes were subsequently purified by immunomagnetic separation using anti CD14-conjugated magnetic microbeads (Miltenyi Biotec) according to the manufacture’s protocol and as previously published (23) ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
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bioRxiv - Immunology 2020Quote: ... Ly6G+ cells were isolated by Neutrophil isolation Kit mouse and F4/80+ cells by Anti-F4/80 Microbeads Ultrapure mouse kit followed the manufactory instructions (MACS, Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec) 85 ...
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bioRxiv - Cancer Biology 2020Quote: ... The NCAM+ Merkel cells in our cell suspension were bound by anti-PSA-NCAM microbeads (Miltenyi Biotec, Cat. 130-097-859) and extracted with the MACS® Column system ...
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from splenocytes of OT-I mice on C57BL/6 background using anti-CD8a microbeads (Miltenyi Biotec), and then fluorescently labeled with carboxyfluorescein succinimidyl ester (CFSE) ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from healthy donor peripheral blood mononuclear cells (PBMCs) by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). moDCs were obtained by culturing monocytes for 5 days with IL-4 and GM-CSF (Peprotech) ...
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bioRxiv - Neuroscience 2019Quote: ... Contaminating mouse splenocytes were depleted from cell preparations by incubating with MACS anti-mouse CD45 magnetic microbeads (Miltenyi Biotec, Auburn, CA) and then washing through magnetic LD Columns as per manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
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bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted MVs were incubated with 10 μl of Biotin-conjugated anti-CD105 (Miltenyi Biotec cat# 130-094-916, RRID: AB_10828348) followed by adding 10 μl of anti-Biotin microbeads (MiltenyiBiotec cat# 130-094-256 ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Immunology 2022Quote: ... Cultures were activated with anti-CD2/CD3/CD28 coated beads at a bead to cell of ratio of 1:1 (Miltenyi Biotec). Cocultures were stained for viability with the LIVE/DEAD Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... to 1×108/ml and Ly6G+ cells were isolated according to the anti-Ly6G MicroBead Kit (#130-092-332, Miltenyi Biotec). The cells were labeled with biotinylated anti-Ly6G antibodies (anti-Ly6G-biotin ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells were enriched on day 9 by depleting all CD43+ and all IgD+ cells with anti-mouse CD43 microbeads (Catalog:130-097-148, Miltenyi Biotec) and biotinylated anti-mouse IgD/anti-biotin microbeads (Catalog:130-090-485 ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16h ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... cells were stained with APC-labeled mMR1-tet and mMR1-tet positive cells were isolated with anti-APC microbeads and LS columns (Miltenyi Biotec). The isolated MAIT cells were intraperitoneally (IP ...
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bioRxiv - Cell Biology 2023Quote: ... Fibroblasts for RT-PCR and Western blot analyses were negatively sorted on Cd11b microbeads and positively sorted for anti-feeder microbeads through LS columns on a QuadroMACS magnet (Miltenyi Biotec). Fibroblasts for RNASeq ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed using RBC lysis buffer before being magnetically sorted using the Anti-Ly-6G MicroBeads UltraPure (Catalog # 130-120-337, Miltenyi Biotec). T cells were distributed into the round bottom 96 well plates at 3000 cells per well and stimulated by CD3/CD28 mAb-coated beads (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution; Miltenyi Biotec), TGF-β1 (5 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2023Quote: ... CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401, Miltenyi Biotec). For CD206-positive (CD206+ ...
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bioRxiv - Immunology 2023Quote: ... and the circulating CD45-PE+ cells were removed from the single cell suspension using magnetic sorting with anti-PE microbeads (Miltenyi Biotec) according to manufacturer’s recommendations (S1A Figure).
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bioRxiv - Immunology 2024Quote: ... staining and single-cell sorting of PvAMA1-specific IgG+ MBCs were performed as follows: B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec) and stained with mouse anti-human CD20 (PE-Cy5.5 ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting single cell suspension was incubated with anti-mouse CD11b (i.e., microglia-specific) magnetic MicroBeads (Miltenyi Biotec, #130–093–634) and microglia were positively selected for via column purification (Miltentyi Biotec ...