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Citations for Miltenyi Biotec :
1701 - 1750 of 1888 citations for Human IgM Anti SARS CoV 2 Spike S1 Antibody CR3022 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Monocytes were isolated using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (Miltenyi Biotec). CD14-positive monocytes were seeded into 12 well plates at 5 × 105 cells/well and differentiated into macrophages by culture in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2020Quote: ... CD14+ Monocytes were further isolated from PBMCs using anti-CD14 magnetic beads (130-050-201, Miltenyi Biotec). CD14+ monocytes were cultured in RPMI 1640 medium (10040CM ...
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bioRxiv - Immunology 2019Quote: ... untouched B cells were enriched using anti-CD43 magnetic beads (MACS) according to manufacturer’s protocol (Miltenyi Biotec) obtaining > 95 % purity ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were positively separated from PBMCs with anti-CD14 magnetic microbeads (Miltenyi Biotec Inc., Auburn, CA, USA).
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bioRxiv - Immunology 2020Quote: ... where mouse neutrophils were enriched from BM by positive magnetic selection with anti–CD11b microbeads (Miltenyi Biotec) before labelling with antibodies ...
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bioRxiv - Immunology 2020Quote: ... The recovered cells were >98% CD14+ as determined by flow cytometry with anti-CD14 Ab (Miltenyi Biotec). The CD14 negative fraction (here PBL ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were purified by positive sorting using anti-CD14-conjugated magnetic microbeads (Miltenyi Biotec, Bergisch Gladsbach, Germany). The recovered cells were >98% CD14+ as determined by flow cytometry with anti-CD14 Ab (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... pro-B cells were positively selected from bone marrow using anti-CD19-labeled magnetic beads (Miltenyi Biotec) and were cultured for 24h hr in Opti-MEM supplemented with 15% FBS (Life Technologies) ...
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bioRxiv - Synthetic Biology 2022Quote: ... whole bone marrow was processed by magnetic selection with anti-CD117 microbeads (Miltenyi Biotec, 130-091-332).
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bioRxiv - Pharmacology and Toxicology 2022Quote: PBMCs were activated in plates coated with 1 μg/mL anti-CD3 mAb (clone: OKT3, Miltenyi Biotec) and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone ...
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bioRxiv - Physiology 2022Quote: ... The SVF pellet was resuspended in FACS buffer and incubated with anti-CD14 magnetic microbeads (Miltenyi Biotec) and F4/80 to isolate adipose tissue macrophages (ATM) ...
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was incubated in extracellular solution containing biotinylated anti-CD29 (0.1 mg/ml, Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... followed by 30 min staining at room temperature with PE-conjugated anti-Flag (clone REA216, Miltenyi Biotec) or pan-HLA class I-specific antibodies (clone W6/32 ...
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bioRxiv - Neuroscience 2023Quote: ... CD11b+ cells were isolated by anti-CD11b MicroBeads (cat # 130-093-634, MACS Miltenyi Biotec, Auburn, CA). CD11b+ cells-removed cell pellets were resuspended and incubated with FcR blocking reagent for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... with the exception that dissociated β-like cells were directly incubated with anti-CD19 MicroBeads (Miltenyi Biotec) instead of a primary antibody.
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bioRxiv - Immunology 2023Quote: ... anti-FITC microbeads (130-148-701) and LS Columns (130-042-401) were purchased from Miltenyi Biotec. FITC Mouse Anti-Human CD4 (555346 ...
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bioRxiv - Immunology 2022Quote: ... and the pellet resuspended prior to the addition of 8.33 μL of anti-APC microbeads (Miltenyi Biotec). Following a 30 minute incubation on ice ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b- positive microglia were magnetically labelled with 20 µl anti-CD11b MicroBeads (# 130- 049-601, Miltenyi Biotec) and incubated for 20 min in the dark at 4°C with gentle shaking ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils were isolated by positive selection using Anti-Ly-6G MicroBeads UltraPure (Miltenyi Biotec; Auburn, CA, USA) and resuspended in RPMI 1640.
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bioRxiv - Immunology 2024Quote: ... was used to fix and permeabilize cells before staining with anti-FoxP3-PE (Clone REA788, Miltenyi Biotec). Cell events were recorded with LSRFortessa (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... also known as LPEC-1 and -6 in previous studies) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) 10-13 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human liver parenchymal primary ECs (EC9) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). ant-CD31 was used for sorting of ECs ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Genomics 2022Quote: ... Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... 2mM ethylenediaminetetracetic acid (EDTA) and PBS and incubated with either CD133-APC or CD133-VioBright FITC antibodies (both monoclonal mouse, Miltenyi Biotec, dilution 1:500) and FcR blocking reagent (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... single cells were obtained as described for flow cytometry from femurs and tibias of 12-week-old male mice and stained with microbead labelled antibodies to CD45 (Miltenyi Biotec, 130-052-301), CD117 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
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bioRxiv - Physiology 2023Quote: ... the cells were mixed with CD45 antibody-coated magnetic beads (CD45 MicroBeads, 130-052-301) and then passed through a MACS LD column (Miltenyi Biotec, 130-042-901) on a MidiMACSTM separator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the PMN suspension was analyzed by flow cytometry (CD3-PerCP-/CD66b-FITC+/CD14-PElow cells > 90%, antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Developmental Biology 2023Quote: ... the flow through eluent from microglia selection served either as a cell type fraction depleted of microglia (denoted as “flow through”) or was used for an additional separation between neuronal and glial fractions by using PSA-NCAM antibody (Miltenyi Biotec, 130-092-966) following the same procedure described for microglia purification.
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bioRxiv - Cancer Biology 2023Quote: CD40 stimulation medium were prepared by incubation of 40 uL of CD40 ligand and 40 uL of cross-linking antibody (Miltenyi Biotec 130-106-196) mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196) ...
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bioRxiv - Cell Biology 2020Quote: The isolation of Thy1 positive EVs is performed by positive selection using anti-CD90 MicroBeads (Miltenyi Biotec, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were purified from mouse bone marrow using anti-Ly6G MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2020Quote: ... AnnexinV-FITC, PI (MBL, code: 4700) and ANTI-PSA-NCAM conjugated-Ab (Miltenyi Biotec, cat. 130-093-273) were added according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... CD14+ cells were positively selected from PBMC using anti-CD14-coated magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Then cells were cultured in 24-well plates (5×105 cells/mL) ...
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bioRxiv - Cancer Biology 2020Quote: ... After washing with cold MACS buffer stained cells were incubated with anti-biotin-microbeads (Miltenyi Biotec, Auburn, CA) for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2021Quote: ... EpSCs were isolated using magnetic-associated cell sorting using Anti-Sca-1 microbeads (Miltenyi Biotec, 130-106-641) and a MACS MultiStand system (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
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bioRxiv - Immunology 2021Quote: ... Naïve mature B cells were isolated from spleen cells by immunomagnetic negative selection with anti-CD43 (Miltenyi Biotec). B cells were cultured in RPMI-1640 medium (Corning ...
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bioRxiv - Cancer Biology 2020Quote: For CSR experiments spleen naive B cells were isolated by immunomagnetic depletion using anti-CD43 beads (Miltenyi Biotec). Purified naïve B cells were cultured at 1.2 x 106 cells/ml in complete RPMI medium supplemented with 10% FBS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). Purity checks and cell counts were performed using a BD Accuri C6 flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: Monocyte isolation was carried out by using Anti-CD14+ Micro Beads (catalog 130-050-201, Miltenyi Biotec, Germany) from PBMCs separated from the blood samples ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from the spleen and peripheral lymph nodes using anti-CD4 microbeads (Miltenyi Biotec) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Physiology 2022Quote: ... samples were resuspended and incubated with 80 μL anti-PE microbeads per 107 cells (Miltenyi Biotec, Cologne, Germany) for 20 minutes at 4°C before Auto MACS cell separation (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked at 37 C for 15 min with 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec). A stimulation with an equimolar amount of DMSO was performed as negative control ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were blocked at 37°C for 15 min with 0.5 μg/ml anti-CD40 mAb (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2019Quote: ... followed by separation with anti-PE magnetic beads using MS columns (both from Miltenyi Biotec, Bergisch Gladbach, Germany). THP1 monocytic cells were maintained in R10.